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RAGE partially mediates cigarettes smoke extract induced cytokine and chemokine production in human bronchial epithelial cells

Alshehri, Mohammed; Brand, Oliver; Alqarni, Abdullah; Pasini, Alice; PANG, LINHUA

Authors

Mohammed Alshehri

Oliver Brand

Abdullah Alqarni

Alice Pasini

LINHUA PANG



Abstract

Chronic obstructive pulmonary disease (COPD) is a common inflammatory lung disease. Cigarette smoke (CS) is known to be the major cause of COPD. RAGE is a 35 kDa protein member belonging to the immunoglobulin superfamily of cell surface receptors. RAGE plays a critical role in airway inflammation. However, the role of RAGE in mediating CSE-induced inflammatory cytokines/chemokines in human bronchial epithelial cells is poorly understood.

Human bronchial epithelial cells (BEAS-2B) were treated with CSE. The effect of RAGE inhibitor (FPS-ZM1) on CSE-induced cytokine/chemokine production was explored to assess the role of RAGE. CXCL5, CCL11, CXCL1, CXCL10, IL-8, and IL-6 were analysed by ELISA. sRAGE secretion and RAGE expression were analysed by ELISA and Western blotting, respectively. Cell viability assay was conducted for CSE and FPS-ZM1.

We found that CXCL1, CXCL5, CXCL10 and CCL11 could not be detected in untreated and CSE-treated cells, but the production of IL-8 and IL-6 was induced by CSE. sRAGE secretion was undetectable in untreated and CSE-treated cells. Interestingly, RAGE was highly expressed in untreated cells, and CSE did not further increase its expression. CSE-induced IL-8 and IL-6 production was partially inhibited by FPS-ZM1. FPS-ZM1 had no effect on RAGE expression.

Our results suggest CS may contribute to the pathogenesis of COPD by modulating pulmonary function through IL-6 and modulating neutrophil-driven airway inflammation through IL-8. RAGE may play a role in mediating CSE-induced IL-8 and IL-6 production from human bronchial epithelial cells. Further studies are needed to knock down RAGE expression to confirm its role.

Citation

Alshehri, M., Brand, O., Alqarni, A., Pasini, A., & PANG, L. (2018). RAGE partially mediates cigarettes smoke extract induced cytokine and chemokine production in human bronchial epithelial cells. European Respiratory Journal, 52(Suppl. 62), doi:10.1183/13993003.congress-2018.PA4246

Journal Article Type Conference Paper
Conference Name ERS International Congress
Start Date Sep 14, 2018
End Date Sep 19, 2018
Acceptance Date Sep 14, 2018
Online Publication Date Sep 14, 2018
Publication Date Sep 14, 2018
Deposit Date Dec 14, 2018
Print ISSN 0903-1936
Publisher European Respiratory Society
Peer Reviewed Peer Reviewed
Volume 52
Issue Suppl. 62
Article Number PA4246
Pages suppl 62:PA4246
DOI https://doi.org/10.1183/13993003.congress-2018.PA4246
Public URL https://nottingham-repository.worktribe.com/output/1416713
Publisher URL http://erj.ersjournals.com/content/52/suppl_62/PA4246