Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Bartley, Kathryn; Deane, David; Percival, Ann; Dry, Inga R.; Grant, Dawn M.; Inglis, Neil F.; Mclean, Kevin; Manson, Erin D.T.; Imrie, Lisa H.J.; Haig, David M.; Lankester, Felix; Russell, George C.

Abstract

Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.

Citation

Bartley, K., Deane, D., Percival, A., Dry, I. R., Grant, D. M., Inglis, N. F., …Russell, G. C. (2014). Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses. Veterinary Microbiology, 173(1-2), 17-26. https://doi.org/10.1016/j.vetmic.2014.07.004

• Journal Article Type: Article
• Acceptance Date: Jul 7, 2014
• Online Publication Date: Jul 15, 2014
• Publication Date: Sep 17, 2014
• Deposit Date: Dec 4, 2018
• Journal: Veterinary Microbiology
• Print ISSN: 0378-1135
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 173
• Issue: 1-2
• Pages: 17-26
• DOI: https://doi.org/10.1016/j.vetmic.2014.07.004
• Keywords: Inflammatory disease; Malignant catarrhal fever; Antibodies; Proteomics; Recombinant expression; Antigen identification
• Public URL: https://nottingham-repository.worktribe.com/output/1362437
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0378113514003277?via%3Dihub