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Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses

Bartley, Kathryn; Deane, David; Percival, Ann; Dry, Inga R.; Grant, Dawn M.; Inglis, Neil F.; Mclean, Kevin; Manson, Erin D.T.; Imrie, Lisa H.J.; Haig, David M.; Lankester, Felix; Russell, George C.


Kathryn Bartley

David Deane

Ann Percival

Inga R. Dry

Dawn M. Grant

Neil F. Inglis

Kevin Mclean

Erin D.T. Manson

Lisa H.J. Imrie

David M. Haig

Felix Lankester

George C. Russell




Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.


Bartley, K., Deane, D., Percival, A., Dry, I. R., Grant, D. M., Inglis, N. F., …Russell, G. C. (2014). Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses. Veterinary Microbiology, 173(1-2), 17-26.

Journal Article Type Article
Acceptance Date Jul 7, 2014
Online Publication Date Jul 15, 2014
Publication Date Sep 17, 2014
Deposit Date Dec 4, 2018
Journal Veterinary Microbiology
Print ISSN 0378-1135
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 173
Issue 1-2
Pages 17-26
Keywords Inflammatory disease; Malignant catarrhal fever; Antibodies; Proteomics; Recombinant expression; Antigen identification
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