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Cooperation between protein kinase A (PKA) and high osmolarity glycerol reponse (HOG) pathways is affecting cell wall carbohydrate mobilization in Aspergillus fumigatus

de Assis, Leandro José; Manfiolli, Adriana; Mattos, Eliciane; Malavazi, Iran; Jacobsen, Ilse D.; Brock, Matthias; Cramer, Robert A.; Thammahong, Arsa; Hagiwara, Daisuke; Nicolas Annick Ries, Laure; Goldman, Gustavo Henrique

Cooperation between protein kinase A (PKA) and high osmolarity glycerol reponse (HOG) pathways is affecting cell wall carbohydrate mobilization in Aspergillus fumigatus Thumbnail


Authors

Leandro José de Assis

Adriana Manfiolli

Eliciane Mattos

Iran Malavazi

Ilse D. Jacobsen

Robert A. Cramer

Arsa Thammahong

Daisuke Hagiwara

Laure Nicolas Annick Ries

Gustavo Henrique Goldman



Abstract

Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents. Upon cell wall stress, cell wall-related sugars need to be synthesized from carbohydrate storage compounds. Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC. These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis. Alterations in glycogen synthesis were observed for the sakA and mpkC deletion mutants, which also displayed alterations in carbohydrate exposure on the cell wall. Carbohydrate mobilization is controlled by SakA interaction with PkaC1 and PkaR, suggesting a putative mechanism where the PkaR regulatory subunit leaves the complex and releases the SakA-PkaC1 complex for activation of enzymes involved in carbohydrate mobilization. This work reveals the communication between the HOG and PKA pathways for carbohydrate mobilization for cell wall construction.

Citation

de Assis, L. J., Manfiolli, A., Mattos, E., Malavazi, I., Jacobsen, I. D., Brock, M., Cramer, R. A., Thammahong, A., Hagiwara, D., Nicolas Annick Ries, L., & Goldman, G. H. (2018). Cooperation between protein kinase A (PKA) and high osmolarity glycerol reponse (HOG) pathways is affecting cell wall carbohydrate mobilization in Aspergillus fumigatus. mBio, 9(6), Article e01952-18. https://doi.org/10.1128/mBio.01952-18

Journal Article Type Article
Acceptance Date Nov 6, 2018
Publication Date Dec 11, 2018
Deposit Date Nov 20, 2018
Publicly Available Date Jan 23, 2019
Journal mBio
Print ISSN 2161-2129
Electronic ISSN 2150-7511
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 9
Issue 6
Article Number e01952-18
DOI https://doi.org/10.1128/mBio.01952-18
Public URL https://nottingham-repository.worktribe.com/output/1288479
Publisher URL https://mbio.asm.org/content/9/6/e01952-18
Contract Date Jan 23, 2019

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