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Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii

Fakae, Lenu B.; Harun, Mohammad S.R.; Ting, Darren Shu Jeng; Dua, Harminder S.; Cave, Gareth W.V.; Zhu, Xing Quan; Stevenson, Carl W.; Elsheikha, Hany M.

Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii Thumbnail


Authors

Lenu B. Fakae

Mohammad S.R. Harun

Darren Shu Jeng Ting

Harminder S. Dua

Gareth W.V. Cave

Xing Quan Zhu



Abstract

We examined the anti-acanthamoebic efficacy of green tea Camellia sinensis solvent extract (SE) or its chemical constituents against Acanthamoeba castellanii by using anti-trophozoite, anti-encystation, and anti-excystation assays. C. sinensis SE (625–5000 µg/mL) inhibited trophozoite replication within 24–72 h. C. sinensis SE exhibited a dose-dependent inhibition of encystation, with a marked cysticidal activity at 2500–5000 µg/mL. Two constituents of C. sinensis, namely epigallocatechin-3-gallate and caffeine, at 100 μM and 200 μM respectively, significantly inhibited both trophozoite replication and encystation. Cytotoxicity analysis showed that 156.25–2500 µg/mL of SE was not toxic to human corneal epithelial cells, while up to 625 µg/mL was not toxic to Madin-Darby canine kidney cells. This study shows the anti-acanthamoebic potential of C. sinensis SE against A. castellanii trophozoites and cysts. Pre-clinical studies are required to elucidate the in vivo efficacy and safety of C. sinensis SE.

Citation

Fakae, L. B., Harun, M. S., Ting, D. S. J., Dua, H. S., Cave, G. W., Zhu, X. Q., …Elsheikha, H. M. (2023). Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii. Acta Tropica, 237, Article 106729. https://doi.org/10.1016/j.actatropica.2022.106729

Journal Article Type Article
Acceptance Date Oct 19, 2022
Online Publication Date Oct 21, 2022
Publication Date Jan 1, 2023
Deposit Date Oct 26, 2022
Publicly Available Date Mar 29, 2024
Journal Acta Tropica
Print ISSN 0001-706X
Electronic ISSN 1873-6254
Publisher Elsevier BV
Peer Reviewed Peer Reviewed
Volume 237
Article Number 106729
DOI https://doi.org/10.1016/j.actatropica.2022.106729
Keywords Infectious Diseases; Parasitology; Insect Science; Veterinary (miscellaneous)
Public URL https://nottingham-repository.worktribe.com/output/12624552
Publisher URL https://www.sciencedirect.com/science/article/pii/S0001706X22004211?via%3Dihub

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