Lenu B. Fakae
Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii
Fakae, Lenu B.; Harun, Mohammad S.R.; Ting, Darren Shu Jeng; Dua, Harminder S.; Cave, Gareth W.V.; Zhu, Xing Quan; Stevenson, Carl W.; Elsheikha, Hany M.
Authors
Mohammad S.R. Harun
Darren Shu Jeng Ting
Harminder S. Dua
Gareth W.V. Cave
Xing Quan Zhu
Dr CARL STEVENSON carl.stevenson@nottingham.ac.uk
Associate Professor
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Abstract
We examined the anti-acanthamoebic efficacy of green tea Camellia sinensis solvent extract (SE) or its chemical constituents against Acanthamoeba castellanii by using anti-trophozoite, anti-encystation, and anti-excystation assays. C. sinensis SE (625–5000 µg/mL) inhibited trophozoite replication within 24–72 h. C. sinensis SE exhibited a dose-dependent inhibition of encystation, with a marked cysticidal activity at 2500–5000 µg/mL. Two constituents of C. sinensis, namely epigallocatechin-3-gallate and caffeine, at 100 μM and 200 μM respectively, significantly inhibited both trophozoite replication and encystation. Cytotoxicity analysis showed that 156.25–2500 µg/mL of SE was not toxic to human corneal epithelial cells, while up to 625 µg/mL was not toxic to Madin-Darby canine kidney cells. This study shows the anti-acanthamoebic potential of C. sinensis SE against A. castellanii trophozoites and cysts. Pre-clinical studies are required to elucidate the in vivo efficacy and safety of C. sinensis SE.
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 19, 2022 |
Online Publication Date | Oct 21, 2022 |
Publication Date | Jan 1, 2023 |
Deposit Date | Oct 26, 2022 |
Publicly Available Date | Oct 27, 2022 |
Journal | Acta Tropica |
Print ISSN | 0001-706X |
Electronic ISSN | 1873-6254 |
Publisher | Elsevier BV |
Peer Reviewed | Peer Reviewed |
Volume | 237 |
Article Number | 106729 |
DOI | https://doi.org/10.1016/j.actatropica.2022.106729 |
Keywords | Infectious Diseases; Parasitology; Insect Science; Veterinary (miscellaneous) |
Public URL | https://nottingham-repository.worktribe.com/output/12624552 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0001706X22004211?via%3Dihub |
Files
1-s2.0-S0001706X22004211-main
(6.6 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by-nc-nd/4.0/
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