Electric field responsive nanotransducers for glioblastoma

Jain, Akhil; Jobson, Isobel; Griffin, Michaela; Rahman, Ruman; Smith, Stuart; Rawson, Frankie J.

Abstract

Background

Electric field therapies such as Tumor Treating Fields (TTFields) have emerged as a bioelectronic treatment for isocitrate dehydrogenase wild-type and IDH mutant grade 4 astrocytoma Glioblastoma (GBM). TTFields rely on alternating current (AC) electric fields (EF) leading to the disruption of dipole alignment and induced dielectrophoresis (DEP) during cytokinesis. Although TTFields have a favourable side effect profile, particularly compared to cytotoxic chemotherapy, survival benefits remain limited (~ 4.9 months) after an extensive treatment regime (20 hours/day for 18 months). The cost of the technology also limits its clinical adoption worldwide. Therefore, the discovery of new technology that can enhance both the therapeutic efficiency and efficacy of these TTFields will be of great benefit to cancer treatment and decrease healthcare costs worldwide.

Methods

In this work, we report the role of electrically conductive gold (GNPs), dielectric silica oxide (SiO2), and semiconductor zinc oxide (ZnO) nanoparticles (NPs) as transducers for enhancing EF mediated anticancer effects on patient derived GBM cells. Physicochemical properties of these NPs were analyzed using spectroscopic, electron microscopy, and light-scattering techniques.

Results

In vitro TTFields studies indicated an enhanced reduction in the metabolic activity of patient-derived Glioma INvasive marginal (GIN 28) and Glioma contrast enhanced core (GCE 28) GBM cells in groups treated with NPs vs. control groups, irrespective of NPs dielectric properties. Our results indicate the inorganic NPs used in this work enhance the intracellular EF effects that could be due to the virtue of bipolar dielectrophoretic and electrophoretic effects.

Conclusions

This work presents preliminary evidence which could help to improve future EF applications for bioelectronic medicine. Furthermore, the merits of spherical morphology, excellent colloidal stability, and low toxicity, make these NPs ideal for future studies for elucidating the detailed mechanism and efficacy upon their delivery in GBM preclinical models.

Citation

Jain, A., Jobson, I., Griffin, M., Rahman, R., Smith, S., & Rawson, F. J. (2022). Electric field responsive nanotransducers for glioblastoma. Bioelectronic Medicine, 8, Article 17. https://doi.org/10.1186/s42234-022-00099-7

• Journal Article Type: Article
• Acceptance Date: Sep 7, 2022
• Online Publication Date: Oct 19, 2022
• Publication Date: Oct 19, 2022
• Deposit Date: Nov 16, 2022
• Publicly Available Date: Nov 18, 2022
• Journal: Bioelectronic Medicine
• Electronic ISSN: 2332-8886
• Publisher: Springer Science and Business Media LLC
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Article Number: 17
• DOI: https://doi.org/10.1186/s42234-022-00099-7
• Keywords: Glioblastoma, Inorganic nanoparticles, Electric Fields, Tumor Treating Fields
• Public URL: https://nottingham-repository.worktribe.com/output/12624524
• Publisher URL: https://bioelecmed.biomedcentral.com/articles/10.1186/s42234-022-00099-7