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Internal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans

Smith, Ken; Cegielski, Jessica; Wilkinson, Daniel; Phillips, Bethan; Boereboom, Catherine; Lund, Jonathan; Atherton, Philip

Internal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans Thumbnail


Authors

Jessica Cegielski

Catherine Boereboom



Abstract

Stable isotope tracer methodologies are becoming increasingly widespread in metabolic research; yet a number of factors restrict their implementation, such as, i.v infusions, multiple cannulae, tissue samples, and significant cost. We recently validated the sensitivity of the orally administered stable isotope tracer deuterium oxide (D2O) for quantifying day‐to‐day changes in muscle protein synthesis (MPS). This method is less invasive, restrictive, and more cost‐effective than traditional amino acid (AA) tracer techniques. In the present study, we hypothesized the sensitivity of our analytical techniques (GC‐Pyrolysis‐IRMS) would permit D2O‐derived measurements of MPS over much shorter periods (i.e., hours) usually only possible using AA‐tracer techniques. We recruited nine males (24 ± 3 year, BMI: 25 ± 3 kg·m−²) into an internally controlled comparison of D2O versus 13C AA‐tracers. The day before the acute study subjects consumed 400 mL D2O, and on the study day, received a primed (0.3 mg·kg−1) continuous (0.6 mg·kg·h−1) i.v infusion of L‐[ring‐13C6]‐phenylalanine to quantify MPS under both: (1) basal [postabsorptive] and; (2) stimulated [postprandial] that is, consumption of 20 g EAA, conditions. Measures of MPS yielded indistinguishable technique differences with respect to EAA, 13C: 0.065 ± 0.004 to 0.089 ± 0.006%·h−1 (P < 0.05) and D2O: 0.050 ± 0.007 to 0.088 ± 0.008%·h−1 (P < 0.05) with qualitatively similar increases. Our findings reveal that acute measurement of MPS, usually only possible using AA‐tracers, are feasible over shorter periods with orally administered D2O when used in tandem with GC‐Pyrolysis‐IRMS. We conclude that this D2O approach provides a less invasive, cost‐effective, and flexible means by which to quantify MPS acutely over several hours.

Citation

Smith, K., Cegielski, J., Wilkinson, D., Phillips, B., Boereboom, C., Lund, J., & Atherton, P. (2015). Internal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans. Physiological Reports, 3(7), Article e12433. https://doi.org/10.14814/phy2.12433

Journal Article Type Article
Acceptance Date May 25, 2015
Publication Date Jul 1, 2015
Deposit Date Sep 6, 2018
Publicly Available Date Feb 7, 2019
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 3
Issue 7
Article Number e12433
DOI https://doi.org/10.14814/phy2.12433
Public URL https://nottingham-repository.worktribe.com/output/1104550
Publisher URL https://physoc.onlinelibrary.wiley.com/doi/full/10.14814/phy2.12433
PMID 26149278

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