A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust)

Kaczmarek, Agata; King, K.M.; West, J.S.; Stevens, M.; Sparkes, Debbie; Dickinson, M.J.

Abstract

Sugar beet rust disease (causal agent Uromyces betae) represents a serious threat to worldwide sugar beet (Beta vulgaris) crops, causing yield losses of up to 10% in the UK. Currently, the disease is managed mainly by application of fungicides after rust disease symptoms appear. Development of a future forecasting system, incorporating data on environmental factors and U. betae inoculum levels, would enable better disease control by more targeted application of fungicides. In this study, we develop a first molecular diagnostic, targeted to cytochrome b DNA sequences and based on loop-mediated isothermal amplification (LAMP) technology, for rapid (

Citation

Kaczmarek, A., King, K., West, J., Stevens, M., Sparkes, D., & Dickinson, M. (2019). A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust). Plant Disease, 103(3), 417-421. https://doi.org/10.1094/PDIS-02-18-0337-RE

• Journal Article Type: Article
• Acceptance Date: Aug 28, 2018
• Online Publication Date: Jan 17, 2019
• Publication Date: Mar 1, 2019
• Deposit Date: Aug 29, 2018
• Journal: Plant Disease
• Print ISSN: 0191-2917
• Electronic ISSN: 1943-7692
• Publisher: American Phytopathological Society
• Peer Reviewed: Peer Reviewed
• Volume: 103
• Issue: 3
• Pages: 417-421
• DOI: https://doi.org/10.1094/PDIS-02-18-0337-RE
• Keywords: Agronomy and Crop Science; Plant Science
• Public URL: https://nottingham-repository.worktribe.com/output/1051349
• Publisher URL: https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-02-18-0337-RE