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A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust)

Kaczmarek, Agata; King, K.M.; West, J.S.; Stevens, M.; Sparkes, Debbie; Dickinson, M.J.

Authors

Agata Kaczmarek

K.M. King

J.S. West

M. Stevens

Debbie Sparkes

M.J. Dickinson



Abstract

Sugar beet rust disease (causal agent Uromyces betae) represents a serious threat to worldwide sugar beet (Beta vulgaris) crops, causing yield losses of up to 10% in the UK. Currently, the disease is managed mainly by application of fungicides after rust disease symptoms appear. Development of a future forecasting system, incorporating data on environmental factors and U. betae inoculum levels, would enable better disease control by more targeted application of fungicides. In this study, we develop a first molecular diagnostic, targeted to cytochrome b DNA sequences and based on loop-mediated isothermal amplification (LAMP) technology, for rapid (

Citation

Kaczmarek, A., King, K., West, J., Stevens, M., Sparkes, D., & Dickinson, M. (2019). A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust). Plant Disease, 103(3), 417-421. https://doi.org/10.1094/PDIS-02-18-0337-RE

Journal Article Type Article
Acceptance Date Aug 28, 2018
Online Publication Date Jan 17, 2019
Publication Date Mar 1, 2019
Deposit Date Aug 29, 2018
Publicly Available Date Mar 29, 2024
Journal Plant Disease
Print ISSN 0191-2917
Electronic ISSN 1943-7692
Publisher American Phytopathological Society
Peer Reviewed Peer Reviewed
Volume 103
Issue 3
Pages 417-421
DOI https://doi.org/10.1094/PDIS-02-18-0337-RE
Keywords Agronomy and Crop Science; Plant Science
Public URL https://nottingham-repository.worktribe.com/output/1051349
Publisher URL https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-02-18-0337-RE