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A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust)

Kaczmarek, Agata; King, K.M.; West, J.S.; Stevens, M.; Sparkes, Debbie; Dickinson, M.J.

Authors

Agata Kaczmarek

K.M. King

J.S. West

M. Stevens



Abstract

Sugar beet rust disease (causal agent Uromyces betae) represents a serious threat to worldwide sugar beet (Beta vulgaris) crops, causing yield losses of up to 10% in the UK. Currently, the disease is managed mainly by application of fungicides after rust disease symptoms appear. Development of a future forecasting system, incorporating data on environmental factors and U. betae inoculum levels, would enable better disease control by more targeted application of fungicides. In this study, we develop a first molecular diagnostic, targeted to cytochrome b DNA sequences and based on loop-mediated isothermal amplification (LAMP) technology, for rapid (

Journal Article Type Article
Publication Date Mar 1, 2019
Journal Plant Disease
Print ISSN 0191-2917
Electronic ISSN 1943-7692
Publisher American Phytopathological Society
Peer Reviewed Peer Reviewed
Volume 103
Issue 3
Pages 417-421
APA6 Citation Kaczmarek, A., King, K., West, J., Stevens, M., Sparkes, D., & Dickinson, M. (2019). A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust). Plant Disease, 103(3), 417-421. https://doi.org/10.1094/PDIS-02-18-0337-RE
DOI https://doi.org/10.1094/PDIS-02-18-0337-RE
Keywords Agronomy and Crop Science; Plant Science
Publisher URL https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-02-18-0337-RE
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