Agata Kaczmarek
A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust)
Kaczmarek, Agata; King, K.M.; West, J.S.; Stevens, M.; Sparkes, Debbie; Dickinson, M.J.
Authors
K.M. King
J.S. West
M. Stevens
Debbie Sparkes
M.J. Dickinson
Abstract
Sugar beet rust disease (causal agent Uromyces betae) represents a serious threat to worldwide sugar beet (Beta vulgaris) crops, causing yield losses of up to 10% in the UK. Currently, the disease is managed mainly by application of fungicides after rust disease symptoms appear. Development of a future forecasting system, incorporating data on environmental factors and U. betae inoculum levels, would enable better disease control by more targeted application of fungicides. In this study, we develop a first molecular diagnostic, targeted to cytochrome b DNA sequences and based on loop-mediated isothermal amplification (LAMP) technology, for rapid (
Citation
Kaczmarek, A., King, K., West, J., Stevens, M., Sparkes, D., & Dickinson, M. (2019). A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust). Plant Disease, 103(3), 417-421. https://doi.org/10.1094/PDIS-02-18-0337-RE
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 28, 2018 |
Online Publication Date | Jan 17, 2019 |
Publication Date | Mar 1, 2019 |
Deposit Date | Aug 29, 2018 |
Journal | Plant Disease |
Print ISSN | 0191-2917 |
Electronic ISSN | 1943-7692 |
Publisher | American Phytopathological Society |
Peer Reviewed | Peer Reviewed |
Volume | 103 |
Issue | 3 |
Pages | 417-421 |
DOI | https://doi.org/10.1094/PDIS-02-18-0337-RE |
Keywords | Agronomy and Crop Science; Plant Science |
Public URL | https://nottingham-repository.worktribe.com/output/1051349 |
Publisher URL | https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-02-18-0337-RE |
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