CpG methylation directly inhibits binding of the human papillomavirus type-16 E2 protein to specific DNA sequences

Thain, Alison; Jenkins, Owen; Clarke, Anthony R.; Gaston, Kevin

Abstract

CpG methylation of the human papillomavirus upstream regulatory region has previously been shown to reduce virus promoter activity. Here, we demonstrate that methylation of the CpG dinucleotides contained within the binding site of the human papillomavirus type 16 E2 protein has a direct effect on the interaction of this protein with DNA. Methylation of both CpG dinucleotides within the E2 site abolishes the binding of E2.

• Journal Article Type: Article
• Acceptance Date: Jul 8, 1996
• Publication Date: 1996-10
• Deposit Date: Nov 24, 2021
• Print ISSN: 0022-538X
• Electronic ISSN: 1098-5514
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 70
• Issue: 10
• Pages: 7233-7235
• DOI: https://doi.org/10.1128/jvi.70.10.7233-7235.1996
• Public URL: https://nottingham-repository.worktribe.com/output/1037885
• Publisher URL: https://journals.asm.org/doi/10.1128/jvi.70.10.7233-7235.1996