Myc and YY1 mediate activation of the Surf-1 promoter in response to serum growth factors

Vernon, Ellen G.; Gaston, Kevin

Abstract

The human Surf-1 and Surf-2 genes are divergently transcribed and share a single bi-directional promoter. The addition of serum growth factors to serum-starved cells activates transcription in the Surf-1 direction, but has no effect on transcription in the Surf-2 direction. Mutations that block the binding of YY1 to a site immediately downstream of the major Surf-1 transcription start point abolish this response to serum factors. Here we show that over-expression of mitogen-activated protein (MAP) kinase phosphatase MKP-1, an inhibitor of the MAP kinase cascade, also blocks the response the Surf-1 promoter to serum factors. YY1 has previously been shown to interact with several transcription factors including Myc. We show that although the Surf-1/Surf-2 promoter does not contain Myc binding sites (E-boxes), Myc over-expression, or the activation of a Myc–oestrogen receptor fusion protein, activates transcription in the Surf-1 direction and that this response to Myc requires a functional YY1 binding site. Our data suggest that the MAP kinase cascade is required for the stimulation of Surf-1 promoter activity and that the Myc-YY1 interaction mediates this response.

Citation

Vernon, E. G., & Gaston, K. (2000). Myc and YY1 mediate activation of the Surf-1 promoter in response to serum growth factors. BBA - Biochimica et Biophysica Acta, 1492(1), 172-179. https://doi.org/10.1016/S0167-4781%2800%2900116-0

• Journal Article Type: Article
• Acceptance Date: Apr 26, 2000
• Online Publication Date: Jun 19, 2000
• Publication Date: Jun 21, 2000
• Deposit Date: Nov 13, 2018
• Journal: BBA - Biochimica et Biophysica Acta
• Print ISSN: 0006-3002
• Electronic ISSN: 0006-3002
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 1492
• Issue: 1
• Pages: 172-179
• DOI: https://doi.org/10.1016/S0167-4781%2800%2900116-0
• Keywords: YY1; Myc; Surf-1; Transcription factor; Growth factor
• Public URL: https://nottingham-repository.worktribe.com/output/1037833
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0167478100001160