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Transcriptional network underpinning ploidy-related elevated leaf potassium in neo-tetraploids

Fischer, Sina; Flis, Paulina; Zhao, Fang Jie; Salt, David E.

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Authors

Paulina Flis

Fang Jie Zhao

David E. Salt



Abstract

Whole-genome duplication generates a tetraploid from a diploid. Newly created tetraploids (neo-tetraploids) of Arabidopsis (Arabidopsis thaliana) have elevated leaf potassium (K), compared to their diploid progenitor. Micro-grafting has previously established that this elevated leaf K is driven by processes within the root. Here, mutational analysis revealed that the K+-uptake transporters K+ TRANSPORTER 1 (AKT1) and HIGH AFFINITY K+ TRANSPORTER 5 (HAK5) are not necessary for the difference in leaf K caused by whole-genome duplication. However, the endodermis and salt overly sensitive and abscisic acid-related signaling were necessary for the elevated leaf K in neo-tetraploids. Contrasting the root transcriptomes of neo-tetraploid and diploid wild-type and mutants that suppress the neo-tetraploid elevated leaf K phenotype allowed us to identify a core set of 92 differentially expressed genes associated with the difference in leaf K between neo-tetraploids and their diploid progenitor. This core set of genes connected whole-genome duplication with the difference in leaf K between neo-tetraploids and their diploid progenitors. The set of genes is enriched in functions such as cell wall and Casparian strip development and ion transport in the endodermis, root hairs, and procambium. This gene set provides tools to test the intriguing idea of recreating the physiological effects of whole-genome duplication within a diploid genome.

Citation

Fischer, S., Flis, P., Zhao, F. J., & Salt, D. E. (2022). Transcriptional network underpinning ploidy-related elevated leaf potassium in neo-tetraploids. Plant Physiology, 190(3), 1715-1730. https://doi.org/10.1093/plphys/kiac360

Journal Article Type Article
Acceptance Date Jul 7, 2022
Online Publication Date Aug 5, 2022
Publication Date Oct 27, 2022
Deposit Date Sep 23, 2022
Publicly Available Date Mar 28, 2024
Journal Plant physiology
Print ISSN 0032-0889
Electronic ISSN 1532-2548
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 190
Issue 3
Article Number kiac360
Pages 1715-1730
DOI https://doi.org/10.1093/plphys/kiac360
Keywords Plant Science; Genetics; Physiology
Public URL https://nottingham-repository.worktribe.com/output/10357070
Publisher URL https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiac360/6656363?login=false

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