The influences of stomatal size and density on rice abiotic stress resilience

Caine, Robert S.; Harrison, Emily L.; Sloan, Jen; Flis, Paulina M.; Fischer, Sina; Khan, Muhammad S.; Nguyen, Phuoc Trong; Nguyen, Lang Thi; Gray, Julie E.; Croft, Holly

Abstract

A warming climate coupled with reductions in water availability and rising salinity are increasingly affecting rice (Oryza sativa) yields. Elevated temperatures combined with vapour pressure deficit (VPD) rises are causing stomatal closure, further reducing plant productivity and cooling. It is unclear what stomatal size (SS) and stomatal density (SD) will best suit all these environmental extremes.

To understand how stomatal differences contribute to rice abiotic stress resilience, we screened the stomatal characteristics of 72 traditionally bred varieties. We found significant variation in SS, SD and calculated anatomical maximal stomatal conductance (gsmax) but did not identify any varieties with SD and gsmax as low as transgenic OsEPF1oe plants.

Traditionally bred varieties with high SD and small SS (resulting in higher gsmax) typically had lower biomasses, and these plants were more resilient to drought than low SD and large SS plants, which were physically larger. None of the varieties assessed were as resilient to drought or salinity as low SD OsEPF1oe transgenic plants. High SD and small SS rice displayed faster stomatal closure during increasing temperature and VPD, but photosynthesis and plant cooling were reduced.

Compromises will be required when choosing rice SS and SD to tackle multiple future environmental stresses.

Citation

Caine, R. S., Harrison, E. L., Sloan, J., Flis, P. M., Fischer, S., Khan, M. S., …Croft, H. (2023). The influences of stomatal size and density on rice abiotic stress resilience. New Phytologist, 237(6), 2180-2195. https://doi.org/10.1111/nph.18704

• Journal Article Type: Article
• Acceptance Date: Dec 5, 2022
• Online Publication Date: Jan 11, 2023
• Publication Date: 2023-03
• Deposit Date: Aug 22, 2023
• Publicly Available Date: Aug 22, 2023
• Journal: New Phytologist
• Print ISSN: 0028-646X
• Electronic ISSN: 1469-8137
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 237
• Issue: 6
• Pages: 2180-2195
• DOI: https://doi.org/10.1111/nph.18704
• Keywords: Climate change; drought; plant water-use; rice; salinity; stomata; temperature; vapour pressure deficit (VPD)
• Public URL: https://nottingham-repository.worktribe.com/output/15940524
• Publisher URL: https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18704