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Calcium induced calcium release during action potential firing in developing inner hair cells

Iosub, Radu; Avitabile, Daniele; Grant, Lisa; Tsaneva-Atanasova, Krasimira; Kennedy, Helen J.

Authors

Radu Iosub

Daniele Avitabile Daniele.Avitabile@nottingham.ac.uk

Lisa Grant

Krasimira Tsaneva-Atanasova

Helen J. Kennedy



Abstract

In the mature auditory system inner hair cells (IHCs) convert sound induced vibrations into electrical signals that are relayed to the CNS via auditory afferents. Before the cochlea can respond to normal sound levels, developing IHCs fire calcium based action potentials that disappear close to the onset of hearing. Action potential firing triggers transmitter release from the immature IHC that in turn generates experience-independent firing in auditory neurons. These early signaling events are thought to be essential for the organization and development of the auditory system and hair cells.
A critical component of the action potential is the rise in intracellular calcium that activates both small conductance potassium channels essential during membrane repolarisation, and triggers transmitter release from the cell. Whether this calcium signal is generated by calcium influx or requires calcium induced calcium release (CICR) is not yet known. IHCs can generate CICR, but to date its physiological role has remained unclear.
Here, we used high and low concentrations of ryanodine to block or enhance CICR to determine whether calcium release from intracellular stores affected action potential waveform, inter-spike interval or changes in membrane capacitance during development of mouse IHCs. Blocking CICR resulted in mixed action potential waveforms with both brief and prolonged oscillations in membrane potential and intracellular calcium. This mixed behaviour is captured well by our mathematical model of IHC electrical activity. We perform two-parameter bifurcation analysis of the model that predicts the dependence of IHCs firing patterns on the level of activation of two parameters, the SK2 channels activation and CICR rate. Our data demonstrate that CICR forms an important component of the calcium signal that shapes action potentials and regulates firing patterns, but is not involved directly in triggering exocytosis. These data provide important insights into the calcium signalling mechanisms involved in early developmental processes.

Citation

Iosub, R., Avitabile, D., Grant, L., Tsaneva-Atanasova, K., & Kennedy, H. J. (2015). Calcium induced calcium release during action potential firing in developing inner hair cells. Biophysical Journal, 108(5), 1003-1012. https://doi.org/10.1016/j.bpj.2014.11.3489

Journal Article Type Article
Acceptance Date Nov 26, 2014
Publication Date Mar 10, 2015
Deposit Date Jan 30, 2015
Publicly Available Date Mar 10, 2015
Journal Biophysical Journal
Print ISSN 0006-3495
Electronic ISSN 0006-3495
Publisher Biophysical Society
Peer Reviewed Peer Reviewed
Volume 108
Issue 5
Pages 1003-1012
DOI https://doi.org/10.1016/j.bpj.2014.11.3489
Keywords Hearing, Cochlea, mathematical modelling, bifurcation analysis, calcium signalling, experience independent-firing, intracellular stores
Public URL http://eprints.nottingham.ac.uk/id/eprint/27883
Publisher URL https://www.sciencedirect.com/science/article/pii/S000634951500082X
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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