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Bursting the bubble on bacterial biofilms: a flow cell methodology

Crusz, Shanika A.; Popat, Roman; Rybtke, Morten Theil; C�mara, Miguel; Givskov, Michael; Tolker-Nielsen, Tim; Diggle, Stephen P.; Williams, Paul


Shanika A. Crusz

Roman Popat

Morten Theil Rybtke

Miguel C�mara

Michael Givskov

Tim Tolker-Nielsen

Stephen P. Diggle

Professor of Molecular Microbiology


The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.


Crusz, S. A., Popat, R., Rybtke, M. T., Cámara, M., Givskov, M., Tolker-Nielsen, T., …Williams, P. (2012). Bursting the bubble on bacterial biofilms: a flow cell methodology. Biofouling, 28(8),

Journal Article Type Article
Publication Date Jan 1, 2012
Deposit Date Apr 16, 2014
Publicly Available Date Apr 16, 2014
Journal Biofouling
Print ISSN 0892-7014
Electronic ISSN 0892-7014
Publisher Taylor & Francis Open
Peer Reviewed Peer Reviewed
Volume 28
Issue 8
Public URL
Publisher URL


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