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Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase

Mahdi, Akeel A.; Briggs, Geoffrey S.; Lloyd, Robert G.

Authors

Akeel A. Mahdi

Geoffrey S. Briggs

Robert G. Lloyd bob.lloyd@nottingham.ac.uk



Abstract

RecG is a DNA translocase that helps to maintain genomic integrity. Initial studies suggested a role in promoting recombination, a possibility consistent with synergism between recG and ruv null alleles and reinforced when the protein was shown to unwind Holliday junctions. In this article we describe novel suppressors of recG and show that the pathology seen without RecG is suppressed on reducing or eliminating PriB, a component of the PriA system for replisome assembly and replication restart. Suppression is conditional, depending on additional mutations that modify ribosomal subunit S6 or one of three subunits of RNA polymerase. The latter suppress phenotypes associated with deletion of priB, enabling the deletion to suppress recG. They include alleles likely to disrupt interactions with transcription anti-terminator, NusA. Deleting priB has a different effect in ruv strains. It provokes abortive recombination and compromises DNA repair in a manner consistent with PriB being required to limit exposure of recombinogenic ssDNA. This synergism is reduced by the RNA polymerase mutations identified. Taken together, the results reveal that RecG curbs a potentially negative effect of proteins that direct replication fork assembly at sites removed from the normal origin, a facility needed to resolve conflicts between replication and transcription.

Citation

Mahdi, A. A., Briggs, G. S., & Lloyd, R. G. (2012). Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase. Molecular Microbiology, 86(3), doi:10.1111/mmi.12010

Journal Article Type Article
Publication Date Nov 1, 2012
Deposit Date May 6, 2014
Publicly Available Date May 6, 2014
Journal Molecular Microbiology
Print ISSN 0950-382X
Electronic ISSN 0950-382X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 86
Issue 3
DOI https://doi.org/10.1111/mmi.12010
Public URL http://eprints.nottingham.ac.uk/id/eprint/3092
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mmi.12010/full
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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