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5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary

Alioui, Anthony; Wheldon, Lee M.; Abakir, Abdulkadir; Ferjentsik, Zoltan; Johnson, Andrew D.; Ruzov, Alexey

Authors

Anthony Alioui

Lee M. Wheldon

Abdulkadir Abakir

Zoltan Ferjentsik

Andrew D. Johnson

Alexey Ruzov Alexey.Ruzov@nottingham.ac.uk



Abstract

5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells and in mouse pre-implantation embryos but no detectable levels of this modification have been reported for somatic tissues to date. Whereas it has been suggested that 5-caC can serve as an intermediate in the process of active demethylation, the function of this form of modified cytosine remains obscure. Here we show that 5-caC is immunochemically detectable in somatic cells of axolotl ovary. We demonstrate that both 5-hmC and 5-caC are localized to the euchromatin in the nuclei of axolotl follicular cells with similar patterns of spatial distribution. Our results suggest that 5-carboxylcytosine may play a distinct functional role in certain biological contexts.

Journal Article Type Article
Publication Date Nov 1, 2012
Journal Nucleus
Print ISSN 1949-1034
Electronic ISSN 1949-1034
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 3
Issue 6
APA6 Citation Alioui, A., Wheldon, L. M., Abakir, A., Ferjentsik, Z., Johnson, A. D., & Ruzov, A. (2012). 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary. Nucleus, 3(6), doi:10.4161/nucl.22799
DOI https://doi.org/10.4161/nucl.22799
Publisher URL https://www.landesbioscience.com/journals/nucleus/article/22799/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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