Construction of a doxycycline inducible adipogenic lentiviral expression system

Liu, Q.; Hill, P.J.; Karamitri, Angeliki; Ryan, K.J.P.; Chen, H.Y.; Lomax, Michael A.

Abstract

To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.

Citation

Liu, Q., Hill, P., Karamitri, A., Ryan, K., Chen, H., & Lomax, M. A. (2013). Construction of a doxycycline inducible adipogenic lentiviral expression system. Plasmid, 69(1), https://doi.org/10.1016/j.plasmid.2012.10.001

• Journal Article Type: Article
• Publication Date: Jan 1, 2013
• Deposit Date: May 14, 2014
• Publicly Available Date: May 14, 2014
• Journal: Plasmid
• Print ISSN: 0147-619X
• Electronic ISSN: 0147-619X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 69
• Issue: 1
• DOI: https://doi.org/10.1016/j.plasmid.2012.10.001
• Keywords: Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte
• Public URL: https://nottingham-repository.worktribe.com/output/1003064
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0147619X12001060