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Construction of a doxycycline inducible adipogenic lentiviral expression system

Liu, Q.; Hill, P.J.; Karamitri, Angeliki; Ryan, K.J.P.; Chen, H.Y.; Lomax, Michael A.


Q. Liu

P.J. Hill

Angeliki Karamitri

K.J.P. Ryan

H.Y. Chen

Michael A. Lomax


To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.


Liu, Q., Hill, P., Karamitri, A., Ryan, K., Chen, H., & Lomax, M. A. (2013). Construction of a doxycycline inducible adipogenic lentiviral expression system. Plasmid, 69(1),

Journal Article Type Article
Publication Date Jan 1, 2013
Deposit Date May 14, 2014
Publicly Available Date May 14, 2014
Journal Plasmid
Print ISSN 0147-619X
Electronic ISSN 0147-619X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 69
Issue 1
Keywords Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte
Public URL
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