Skip to main content

Research Repository

See what's under the surface

Advanced Search

Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion

Rao, Wei; Celiz, Adam D.; Scurr, David J.; Alexander, Morgan R.; Barrett, David A.

Authors

Wei Rao

Adam D. Celiz

David J. Scurr

Morgan R. Alexander

David A. Barrett



Abstract

The detection and identification of proteins adsorbed onto biomaterial surfaces under ambient conditions has significant experimental advantages but has proven to be difficult to achieve with conventional measuring technologies. In this study, we present an adaptation of desorption electrospray ionization (DESI) and liquid extraction surface analysis (LESA) mass spectrometry (MS) coupled with in-situ surface tryptic digestion to identify protein species from a biomaterial surface. Cytochrome c, myoglobin, and BSA in a combination of single and mixture spots were printed in an array format onto Permanox slides, followed by in-situ surface digestion and detection via MS. Automated tandem MS performed on surface peptides was able to identify the proteins via MASCOT. Limits of detection were determined for DESI-MS and a comparison of DESI and LESA-MS peptide spectra characteristics and sensitivity was made. DESI-MS images of the arrays were produced and analyzed with imaging multivariate analysis to automatically separate peptide peaks for each of the proteins within a mixture into distinct components. This is the first time that DESI and LESA-MS have been used for the in-situ detection of surface digested proteins on biomaterial surfaces and presents a promising proof of concept for the use of ambient MS in the rapid and automated analysis of surface proteins.

Journal Article Type Article
Publication Date Dec 1, 2013
Journal Journal of The American Society for Mass Spectrometry
Print ISSN 1044-0305
Electronic ISSN 1044-0305
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 24
Issue 12
APA6 Citation Rao, W., Celiz, A. D., Scurr, D. J., Alexander, M. R., & Barrett, D. A. (2013). Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion. Journal of The American Society for Mass Spectrometry, 24(12), doi:10.1007/s13361-013-0737-3
DOI https://doi.org/10.1007/s13361-013-0737-3
Keywords LESA-MS ; DESI-MS ; Ambient MS Protein arrays ; In-situ digestion ; MS imaging ; MCR analysis
Publisher URL http://link.springer.com/article/10.1007%2Fs13361-013-0737-3
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

Files

David_Barrett--Ambient_DESI.pdf (606 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





You might also like



Downloadable Citations

;