Outputs (22)

PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus (2016)
Journal Article
Blanchard, A. M., Egan, S. A., Emes, R. D., Warry, A., & Leigh, J. A. (2016). PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus. Frontiers in Microbiology, 7(1645), https://doi.org/10.3389/fmicb.2016.01645

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al., 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related ba... Read More about PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus.

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System (2015)
Journal Article
Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, Article e139. https://doi.org/10.3389/fgene.2015.00139

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genom... Read More about Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System.