Outputs (212)

Co-assembling living material as an in vitro lung epithelial infection model (2023)
Journal Article
Wu, Y., Romero, M., Robertson, S. N., Fenn, S., Fisher, L., Willingham, I., Martinez Pomares, L., Ligorio, C., Hill, J., Bu, W., Zhou, Z., Wildman, R. D., Ghaemmaghami, A. M., Sun, H., Sun, J., Cámara, M., & Mata, A. (2024). Co-assembling living material as an in vitro lung epithelial infection model. Matter, 7(1), 216-236. https://doi.org/10.1016/j.matt.2023.10.029

Biofilms are robust living 3D materials that play key roles in nature but also cause major problems, such as tolerance to antibiotic treatment. Recreation of these living structures in vitro is critical to understand their biology and develop solutio... Read More about Co-assembling living material as an in vitro lung epithelial infection model.

Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity (2023)
Journal Article
Kirchhofer, S. B., Lim, V. J. Y., Ernst, S., Karsai, N., Julia, R. G., Canals, M., Kolb, P., & Bünemann, M. (2023). Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity. eLife, 12, Article e91291. https://doi.org/10.7554/eLife.91291

The µ opioid receptor (MOR) is the key target for analgesia, but the application of opioids is accompanied by several issues. There is a wide range of opioid analgesics, differing in their chemical structure and their properties of receptor activatio... Read More about Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity.

A sporulation signature protease is required for assembly of the spore surface layers, germination and host colonization in Clostridioides difficile (2023)
Journal Article
Marini, E., Olivença, C., Ramalhete, S., Aguirre, A. M., Ingle, P., Melo, M. N., Antunes, W., Minton, N. P., Hernandez, G., Cordeiro, T. N., Sorg, J. A., Serrano, M., & Henriques, A. O. (2023). A sporulation signature protease is required for assembly of the spore surface layers, germination and host colonization in Clostridioides difficile. PLoS Pathogens, 19(11), Article e1011741. https://doi.org/10.1371/journal.ppat.1011741

A genomic signature for endosporulation includes a gene coding for a protease, YabG, which in the model organism Bacillus subtilis is involved in assembly of the spore coat. We show that in the human pathogen Clostridioidesm difficile, YabG is critic... Read More about A sporulation signature protease is required for assembly of the spore surface layers, germination and host colonization in Clostridioides difficile.

Design, construction, and functional characterization of a tRNA neochromosome in yeast (2023)
Journal Article
Schindler, D., Walker, R. S., Jiang, S., Brooks, A. N., Wang, Y., Müller, C. A., Cockram, C., Luo, Y., García, A., Schraivogel, D., Mozziconacci, J., Pena, N., Assari, M., Sánchez Olmos, M. D. C., Zhao, Y., Ballerini, A., Blount, B. A., Cai, J., Ogunlana, L., Liu, W., …Cai, Y. (2023). Design, construction, and functional characterization of a tRNA neochromosome in yeast. Cell, 186(24), 5237-5253.e22. https://doi.org/10.1016/j.cell.2023.10.015

Here, we report the design, construction, and characterization of a tRNA neochromosome, a designer chromosome that functions as an additional, de novo counterpart to the native complement of Saccharomyces cerevisiae. Intending to address one of the c... Read More about Design, construction, and functional characterization of a tRNA neochromosome in yeast.

Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics (2023)
Journal Article
Blount, B. A., Lu, X., Driessen, M. R. M., Jovicevic, D., Sanchez, M. I., Ciurkot, K., Zhao, Y., Lauer, S., McKiernan, R. M., Gowers, G.-O. F., Sweeney, F., Fanfani, V., Lobzaev, E., Palacios-Flores, K., Walker, R. S. K., Hesketh, A., Cai, J., Oliver, S. G., Cai, Y., Stracquadanio, G., …Ellis, T. (2023). Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics. Cell Genomics, 3(11), Article 100418. https://doi.org/10.1016/j.xgen.2023.100418

We describe construction of the synthetic yeast chromosome XI (synXI) and reveal the effects of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project (Sc2.0) chromosome was assembled from synthesized DNA fragments before CRIS... Read More about Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics.

Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing (2023)
Journal Article
Green, M. J., Murray, E. J., Williams, P., Ghaemmaghami, A. M., Aylott, J. W., & Williams, P. M. (2023). Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing. International Journal of Molecular Sciences, 24(21), Article 15997. https://doi.org/10.3390/ijms242115997

Bacterial contamination during space missions is problematic for human health and damages filters and other vital support systems. Staphylococcus aureus is both a human commensal and an opportunistic pathogen that colonizes human tissues and causes a... Read More about Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing.

Adaptation to sorbic acid in low sugar promotes resistance of yeast to the preservative (2023)
Journal Article
Harvey, H. J., Hendry, A., Chirico, M., Archer, D. B., & V. Avery, S. (2023). Adaptation to sorbic acid in low sugar promotes resistance of yeast to the preservative. Heliyon, 9(11), Article e22057. https://doi.org/10.1016/j.heliyon.2023.e22057

The weak acid sorbic acid is a common preservative used in soft drink beverages to control microbial spoilage. Consumers and industry are increasingly transitioning to low-sugar food formulations, but potential impacts of reduced sugar on sorbic acid... Read More about Adaptation to sorbic acid in low sugar promotes resistance of yeast to the preservative.

Invariant NKT cells are more abundant in peanut-allergic adults and a subset of CD8 + iNKT cells are depleted after peanut oil exposure (2023)
Journal Article
Hopkins, G. V., Cochrane, S., Onion, D., & Fairclough, L. C. (2023). Invariant NKT cells are more abundant in peanut-allergic adults and a subset of CD8 + iNKT cells are depleted after peanut oil exposure. Frontiers in Immunology, 14, Article 1293158. https://doi.org/10.3389/fimmu.2023.1293158

Introduction:

Peanut allergy is one of the most prevalent food allergies globally. Currently, most research into the mechanisms involved in protein allergy focuses on the protein allergens under investigation, and information on the function of a... Read More about Invariant NKT cells are more abundant in peanut-allergic adults and a subset of CD8 + iNKT cells are depleted after peanut oil exposure.

COMPARISON BETWEEN SANGER, ILLUMINA AND NANOPORE SEQUENCING EVIDENCING INTRA-HOST VARIATION OF FELINE LEUKEMIA VIRUS THAT INFECTS DOMESTIC CATS. (2023)
Preprint / Working Paper
Castillo-Aliaga, C., Blanchard, A. M., Castro-Seriche, S., Hidalgo-Hermoso, E., Jerez-Morales, A., Loose, M. W., & Tarlinton, R. E. (2023). COMPARISON BETWEEN SANGER, ILLUMINA AND NANOPORE SEQUENCING EVIDENCING INTRA-HOST VARIATION OF FELINE LEUKEMIA VIRUS THAT INFECTS DOMESTIC CATS

Knowledge of Feline Leukemia Virus (FeLV) sequence variation has mainly been developed using Sanger sequencing methods. However, advances in next generation sequencing methods and their broad use in laboratories has been changing our understanding of... Read More about COMPARISON BETWEEN SANGER, ILLUMINA AND NANOPORE SEQUENCING EVIDENCING INTRA-HOST VARIATION OF FELINE LEUKEMIA VIRUS THAT INFECTS DOMESTIC CATS..

The IUPHAR/BPS Guide to PHARMACOLOGY in 2024 (2023)
Journal Article
Harding, S. D., Armstrong, J. F., Faccenda, E., Southan, C., Alexander, S. H., Davenport, A. P., Spedding, M., & Davies, J. A. (2024). The IUPHAR/BPS Guide to PHARMACOLOGY in 2024. Nucleic Acids Research, 52(D1), D1438-D1449. https://doi.org/10.1093/nar/gkad944

The IUPHAR/BPS Guide to PHARMACOLOGY (GtoPdb; https://www.guidetopharmacology.org) is an open-access, expert-curated, online database that provides succinct overviews and key references for pharmacological targets and their recommended experimental l... Read More about The IUPHAR/BPS Guide to PHARMACOLOGY in 2024.