Dr DARREN WELLS's Outputs (38)

Root metaxylem area influences drought tolerance and transpiration in pearl millet in a soil texture dependent manner (2024)
Preprint / Working Paper
Affortit, P., Faye, A., Jones, D. H., Benson, E., Sine, B., Burridge, J., Ndoye, M. S., Barry, L., Moukouanga, D., Barnard, S., Bhosale, R., Pridmore, T., Gantet, P., Vadez, V., Cubry, P., Kane, N., Bennett, M., Atkinson, J. A., Laplaze, L., Wells, D. M., & Grondin, A. (2024). Root metaxylem area influences drought tolerance and transpiration in pearl millet in a soil texture dependent manner

Pearl millet is a key cereal for food security in drylands but its yield is strongly impacted by drought. We investigated how root anatomical traits contribute to mitigating the effects of vegetative drought stress in pearl millet.

We examined ass... Read More about Root metaxylem area influences drought tolerance and transpiration in pearl millet in a soil texture dependent manner.

A novel root hair mutant, srh1, affects root hair elongation and reactive oxygen species levels in wheat (2024)
Journal Article
Tsang, I., Thomelin, P., Ober, E. S., Rawsthorne, S., Atkinson, J. A., Wells, D. M., Percival-Alwyn, L., Leigh, F. J., & Cockram, J. (2024). A novel root hair mutant, srh1, affects root hair elongation and reactive oxygen species levels in wheat. Frontiers in Plant Science, 15, Article 1490502. https://doi.org/10.3389/fpls.2024.1490502

Background: Root hairs are single-celled projections on root surfaces, critical for water and nutrient uptake. Here, we describe the first short root hair mutant in wheat (Triticum aestivum L.), identified in a mutagenized population and termed here... Read More about A novel root hair mutant, srh1, affects root hair elongation and reactive oxygen species levels in wheat.

Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet (2024)
Journal Article
de la Fuente, C., Grondin, A., Sine, B., Debieu, M., Belin, C., Hajjarpoor, A., Atkinson, J. A., Passot, S., Salson, M., Orjuela, J., Tranchant-Dubreuil, C., Brossier, J.-R., Steffen, M., Morgado, C., Dinh, H. N., Pandey, B. K., Darmau, J., Champion, A., Petitot, A.-. S., Barrachina, C., …Laplaze, L. (2024). Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet. eLife, https://doi.org/10.7554/eLife.86169

Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet’s early root sy... Read More about Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet.

Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet (2024)
Journal Article
de la Fuente, C., Grondin, A., Sine, B., Debieu, M., Belin, C., Hajjarpoor, A., Atkinson, J. A., Passot, S., Salson, M., Orjuela, J., Tranchant-Dubreuil, C., Brossier, J.-R., Steffen, M., Morgado, C., Dinh, H. N., Pandey, B. K., Darmau, J., Champion, A., Petitot, A.-S., Barrachina, C., …Laplaze, L. (2024). Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet. eLife, 12, Article RP86169. https://doi.org/10.7554/elife.86169.3

Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet’s early root sy... Read More about Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet.

Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet (2023)
Preprint / Working Paper
de la Fuente, C., Grondin, A., Sine, B., Debieu, M., Belin, C., Hajjarpoor, A., Atkinson, J. A., Passot, S., Salson, M., Orjuela, J., Tranchant-Dubreuil, C., Brossier, J.-R., Steffen, M., Morgado, C., Dinh, H. N., Pandey, B. K., Darmau, J., Champion, A., Petitot, A.-. S., Barrachina, C., …Laplaze, L. Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet

Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet’s early root sy... Read More about Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet.

Chlorophyll fluorescence-based high-throughput phenotyping facilitates the genetic dissection of photosynthetic heat tolerance in African (Oryza glaberrima) and Asian (Oryza sativa) rice (2023)
Journal Article
Robson, J. K., Ferguson, J. N., McAusland, L., Atkinson, J. A., Tranchant-Dubreuil, C., Cubry, P., Sabot, F., Wells, D. M., Price, A. H., Wilson, Z. A., & Murchie, E. H. (2023). Chlorophyll fluorescence-based high-throughput phenotyping facilitates the genetic dissection of photosynthetic heat tolerance in African (Oryza glaberrima) and Asian (Oryza sativa) rice. Journal of Experimental Botany, 74(17), 5181-5197. https://doi.org/10.1093/jxb/erad239

Rising temperatures and extreme heat events threaten rice production. Half of the global population relies on rice for basic nutrition, and therefore developing heat-tolerant rice is essential. During vegetative development, reduced photosynthetic ra... Read More about Chlorophyll fluorescence-based high-throughput phenotyping facilitates the genetic dissection of photosynthetic heat tolerance in African (Oryza glaberrima) and Asian (Oryza sativa) rice.

Loss of ancestral function in duckweed roots is accompanied by progressive anatomical reduction and a re-distribution of nutrient transporters (2023)
Journal Article
Ware, A., Jones, D. H., Flis, P., Chrysanthou, E., Smith, K. E., Kümpers, B. M., Yant, L., Atkinson, J. A., Wells, D. M., Bhosale, R., & Bishopp, A. (2023). Loss of ancestral function in duckweed roots is accompanied by progressive anatomical reduction and a re-distribution of nutrient transporters. Current Biology, 33(9), 1795-1802. https://doi.org/10.1016/j.cub.2023.03.025

Organ loss occurs frequently during plant and animal evolution. Sometimes, non-functional organs are retained through evolution. Vestigial organs are defined as genetically determined structures that have lost their ancestral (or salient) function. D... Read More about Loss of ancestral function in duckweed roots is accompanied by progressive anatomical reduction and a re-distribution of nutrient transporters.

Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet (2023)
Preprint / Working Paper
de la Fuente, C., Grondin, A., Sine, B., Debieu, M., Belin, C., Hajjarpoor, A., Atkinson, J. A., Passot, S., Salson, M., Orjuela, J., Tranchant-Dubreuil, C., Brossier, J.-R., Steffen, M., Morgado, C., Dinh, H. N., Pandey, B. K., Darmau, J., Champion, A., Petitot, A.-. S., Barrachina, C., …Laplaze, L. (2024). Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet

Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet’s early root sy... Read More about Glutaredoxin regulation of primary root growth confers early drought stress tolerance in pearl millet.

Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat (2022)
Journal Article
Kareem, S. H., Hawkesford, M. J., DeSilva, J., Weerasinghe, M., Wells, D. M., Pound, M. P., Atkinson, J. A., & Foulkes, M. J. (2022). Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat. European Journal of Agronomy, 140, Article 126603. https://doi.org/10.1016/j.eja.2022.126603

Root system architecture (RSA) is important in optimizing the use of nitrogen. High-throughput phenotyping techniques may be used to study root system architecture traits under controlled environments. A root phenotyping platform, consisting of germi... Read More about Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat.

Root system size and root hair length are key phenes for nitrate acquisition and biomass production across natural variation in Arabidopsis (2022)
Journal Article
De Pessemier, J., Moturu, T. R., Nacry, P., Ebert, R., De Gernier, H., Tillard, P., Swarup, K., Wells, D. M., Haseloff, J., Murray, S. C., Bennett, M. J., Inze, D., Vincent, C. I., & Hermans, C. (2022). Root system size and root hair length are key phenes for nitrate acquisition and biomass production across natural variation in Arabidopsis. Journal of Experimental Botany, 73(11), 3569-3583

The role of root phenes in nitrogen (N) acquisition and biomass production was evaluated in 10 contrasting natural accessions of Arabidopsis thaliana L. Seedlings were grown on vertical agar plates with two different nitrate supplies. The low N treat... Read More about Root system size and root hair length are key phenes for nitrate acquisition and biomass production across natural variation in Arabidopsis.

Identification of QTL and underlying genes for root system architecture associated with nitrate nutrition in hexaploid wheat (2022)
Journal Article
GRIFFITHS, M., ATKINSON, J. A., Gardiner, L. J., SWARUP, R., POUND, M. P., WILSON, M. H., BENNETT, M. J., & WELLS, D. M. (2022). Identification of QTL and underlying genes for root system architecture associated with nitrate nutrition in hexaploid wheat. Journal of Integrative Agriculture, 21(4), 917-932. https://doi.org/10.1016/s2095-3119%2821%2963700-0

The root system architecture (RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat (Triticum aestivum L.... Read More about Identification of QTL and underlying genes for root system architecture associated with nitrate nutrition in hexaploid wheat.

X-ray CT reveals 4D root system development and lateral root responses to nitrate in soil (2022)
Journal Article
Griffiths, M., Mellor, N., Sturrock, C. J., Atkinson, B. S., Johnson, J., Mairhofer, S., York, L. M., Atkinson, J. A., Soltaninejad, M., Foulkes, J. F., Pound, M. P., Mooney, S. J., Pridmore, T. P., Bennett, M. J., & Wells, D. M. (2022). X-ray CT reveals 4D root system development and lateral root responses to nitrate in soil. Plant Phenome Journal, 5(1), Article e20036. https://doi.org/10.1002/ppj2.20036

The spatial arrangement of the root system, termed root system architecture, is important for resource acquisition as it directly affects the soil zone explored. Methods for phenotyping roots are mostly destructive, which prevents analysis of roots o... Read More about X-ray CT reveals 4D root system development and lateral root responses to nitrate in soil.

Dual expression and anatomy lines allow simultaneous visualization of gene expression and anatomy (2021)
Journal Article
Kümpers, B. M. C., Han, J., Vaughan-Hirsch, J., Redman, N., Ware, A., Atkinson, J. A., Leftley, N., Janes, G., Castiglione, G., Tarr, P. T., Pyke, K., Voß, U., Wells, D. M., & Bishopp, A. (2022). Dual expression and anatomy lines allow simultaneous visualization of gene expression and anatomy. Plant Physiology, 188(1), 56-69. https://doi.org/10.1093/plphys/kiab503

Studying the developmental genetics of plant organs, requires following gene expression in specific tissues. To facilitate this, we have developed the Dual Expression Anatomy Lines (DEAL), which incorporate a red plasma membrane marker alongside a fl... Read More about Dual expression and anatomy lines allow simultaneous visualization of gene expression and anatomy.

Non-invasive hydrodynamic imaging in plant roots at cellular resolution (2021)
Journal Article
Pascut, F. C., Couvreur, V., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., Calvo-Polanco, M., Casimiro, I., Maurel, C., Salt, D. E., Draye, X., Wells, D. M., Bennett, M. J., & Webb, K. F. (2021). Non-invasive hydrodynamic imaging in plant roots at cellular resolution. Nature Communications, 12, Article 4682. https://doi.org/10.1038/s41467-021-24913-z

A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelli... Read More about Non-invasive hydrodynamic imaging in plant roots at cellular resolution.

Positioning the Root Elongation Zone Is Saltatory and Receives Input from the Shoot (2020)
Journal Article
Baskin, T. I., Preston, S., Zelinsky, E., Yang, X., Elmali, M., Bellos, D., Wells, D. M., & Bennett, M. J. (2020). Positioning the Root Elongation Zone Is Saltatory and Receives Input from the Shoot. iScience, 23(7), Article 101309. https://doi.org/10.1016/j.isci.2020.101309

In the root, meristem and elongation zone lengths remain stable, despite growth and division of cells. To gain insight into zone stability, we imaged individual Arabidopsis thaliana roots through a horizontal microscope, and used image analysis to ob... Read More about Positioning the Root Elongation Zone Is Saltatory and Receives Input from the Shoot.

Low-cost automated vectors and modular environmental sensors for plant phenotyping (2020)
Journal Article
Bagley, S. A., Atkinson, J. A., Hunt, H., Wilson, M. H., Pridmore, T. P., & Wells, D. M. (2020). Low-cost automated vectors and modular environmental sensors for plant phenotyping. Sensors, 20(11), Article 3319. https://doi.org/10.3390/s20113319

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. High-throughput plant phenotyping in controlled environments (growth chambers and glasshouses) is often delivered via large, expensive installations, leading to limited access and the increase... Read More about Low-cost automated vectors and modular environmental sensors for plant phenotyping.

RootNav 2.0: Deep learning for automatic navigation of complex plant root architectures (2019)
Journal Article
Yasrab, R., Atkinson, J. A., Wells, D. M., French, A. P., Pridmore, T. P., & Pound, M. P. (2019). RootNav 2.0: Deep learning for automatic navigation of complex plant root architectures. GigaScience, 8(11), Article giz123. https://doi.org/10.1093/gigascience/giz123

BACKGROUND: In recent years quantitative analysis of root growth has become increasingly important as a way to explore the influence of abiotic stress such as high temperature and drought on a plant's ability to take up water and nutrients. Segmentat... Read More about RootNav 2.0: Deep learning for automatic navigation of complex plant root architectures.

Supporting data for "RootNav 2.0: Deep Learning for Automatic Navigation of Complex Plant Root Architectures" (2019)
Data
French, A., Wells, D. M., Atkinson, J., Pound, M., Yasrab, R., & Pridmore, T. (2019). Supporting data for "RootNav 2.0: Deep Learning for Automatic Navigation of Complex Plant Root Architectures". [Data]. https://doi.org/10.5524/100651

We present a new image analysis approach that provides fully-automatic extraction of complex root system architectures from a range of plant species in varied imaging setups. Driven by modern deep-learning approaches, RootNav 2.0 replaces previously... Read More about Supporting data for "RootNav 2.0: Deep Learning for Automatic Navigation of Complex Plant Root Architectures".

Identification of nitrogen-dependent QTL and underlying genes for root system architecture in hexaploid wheat (2019)
Other
Griffiths, M., Atkinson, J. A., Gardiner, L.-J., Swarup, R., Pound, M. P., Wilson, M. H., Bennett, M. J., & Wells, D. M. (2019). Identification of nitrogen-dependent QTL and underlying genes for root system architecture in hexaploid wheat

The root system architecture (RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource dependent response in wheat (Triticum aestivum L.... Read More about Identification of nitrogen-dependent QTL and underlying genes for root system architecture in hexaploid wheat.

The circadian clock rephases during lateral root organ initiation in Arabidopsis thaliana. (2018)
Journal Article
Voß, U., Wilson, M. H., Kenobi, K., Gould, P. D., Robertson, F. C., Peer, W. A., Lucas, M., Swarup, K., Casimiro, I., Holman, T. J., Wells, D. M., Péret, B., Goh, T., Fukaki, H., Hodgman, T. C., Laplaze, L., Halliday, K. J., Ljung, K., Murphy, A. S., Hall, A. J., …Bennett, M. J. (2018). The circadian clock rephases during lateral root organ initiation in Arabidopsis thaliana. Nature Communications, https://doi.org/10.17863/CAM.26324