X-ray CT reveals 4D root system development and lateral root responses to nitrate in soil

Griffiths, Marcus; Mellor, Nathan; Sturrock, Craig J.; Atkinson, Brian S.; Johnson, James; Mairhofer, Stefan; York, Larry M.; Atkinson, Jonathan A.; Soltaninejad, Mohammadreza; Foulkes, John F.; Pound, Michael P.; Mooney, Sacha J.; Pridmore, Tony P.; Bennett, Malcolm J.; Wells, Darren M.

Abstract

The spatial arrangement of the root system, termed root system architecture, is important for resource acquisition as it directly affects the soil zone explored. Methods for phenotyping roots are mostly destructive, which prevents analysis of roots over time as they grow. Here, we used X-ray microcomputed tomography (μCT) to non-invasively characterize wheat (Triticum aestivum L.) seedling root development across time under high and low nitrate nutrition. Roots were imaged multiple times with the 3D models co-aligned and timestamped in the architectural plant model OpenSimRoot for subsequent root growth and nitrate uptake simulations. Through 4D imaging, we found that lateral root traits were highly responsive to nitrate limitation in soil with greater lateral root length under low N. The root growth model using all μCT root scans was comparable to a parameterized model using only the final root scan in the series. In a second μCT experiment, root growth and nitrate uptake simulations of candidate wheat genotypes found significant root growth and uptake differences between lines. A high nitrate uptake wheat line selected from field data had a greater lateral root count and length at seedling growth stage compared with a low uptake line.

Citation

Griffiths, M., Mellor, N., Sturrock, C. J., Atkinson, B. S., Johnson, J., Mairhofer, S., York, L. M., Atkinson, J. A., Soltaninejad, M., Foulkes, J. F., Pound, M. P., Mooney, S. J., Pridmore, T. P., Bennett, M. J., & Wells, D. M. (2022). X-ray CT reveals 4D root system development and lateral root responses to nitrate in soil. Plant Phenome Journal, 5(1), Article e20036. https://doi.org/10.1002/ppj2.20036

• Journal Article Type: Article
• Acceptance Date: Jan 21, 2022
• Online Publication Date: Feb 25, 2022
• Publication Date: Feb 25, 2022
• Deposit Date: Jan 28, 2022
• Publicly Available Date: Feb 25, 2022
• Journal: Plant Phenome Journal
• Electronic ISSN: 2578-2703
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 1
• Article Number: e20036
• DOI: https://doi.org/10.1002/ppj2.20036
• Public URL: https://nottingham-repository.worktribe.com/output/7344620
• Publisher URL: https://acsess.onlinelibrary.wiley.com/doi/10.1002/ppj2.20036