Non-invasive hydrodynamic imaging in plant roots at cellular resolution

Pascut, Flavius C.; Couvreur, Valentin; Dietrich, Daniela; Leftley, Nicky; Reyt, Guilhem; Boursiac, Yann; Calvo-Polanco, Monica; Casimiro, Ilda; Maurel, Christophe; Salt, David E.; Draye, Xavier; Wells, Darren M.; Bennett, Malcolm J.; Webb, Kevin F.

Abstract

A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’.

Citation

Pascut, F. C., Couvreur, V., Dietrich, D., Leftley, N., Reyt, G., Boursiac, Y., …Webb, K. F. (2021). Non-invasive hydrodynamic imaging in plant roots at cellular resolution. Nature Communications, 12(1), 1-7. https://doi.org/10.1038/s41467-021-24913-z

• Journal Article Type: Article
• Acceptance Date: Jul 7, 2021
• Online Publication Date: Aug 3, 2021
• Publication Date: Dec 1, 2021
• Deposit Date: Jul 19, 2021
• Publicly Available Date: Aug 4, 2021
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 1
• Article Number: 1682
• Pages: 1-7
• DOI: https://doi.org/10.1038/s41467-021-24913-z
• Public URL: https://nottingham-repository.worktribe.com/output/5805726
• Publisher URL: https://www.nature.com/articles/s41467-021-24913-z