All Outputs (5)

Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis (2024)
Journal Article
Cleary, S. E., Hall, S. J., Galan-Bataller, R., Lurshay, T. C., Hancox, C., Williamson, J. J., Heap, J. T., Reeve, H. A., & Morra, S. (2024). Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis. ChemCatChem, 16(16), Article e202400193. https://doi.org/10.1002/cctc.202400193

The enzyme CbA5H, a [FeFe]-hydrogenase from Clostridium beijerinckii, has previously been shown to survive exposure to oxygen, making it a promising candidate for biotechnological applications. Thus far [NiFe]-hydrogenases are typically considered fo... Read More about Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis.

Efficient bio-production of citramalate using an engineered Escherichia coli strain (2017)
Journal Article
Webb, J. P., Arnold, S. A., Baxter, S., Hall, S. J., Eastham, G., & Stephens, G. (2018). Efficient bio-production of citramalate using an engineered Escherichia coli strain. Microbiology, 164(2), 133-141. https://doi.org/10.1099/mic.0.000581

Citramalic acid is a central intermediate in a combined biocatalytic and chemocatalytic route to produce bio-based methylmethacrylate, the monomer used to manufacture Perspex and other high performance materials. We developed an engineered E. coli st... Read More about Efficient bio-production of citramalate using an engineered Escherichia coli strain.

Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes (2017)
Journal Article
Mordaka, P. M., Hall, S. J., Minton, N. P., & Stephens, G. (in press). Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes. Microbiology, https://doi.org/10.1099/mic.0.000568

‘Ene’-reductases have attracted significant attention for the preparation of chemical intermediates and biologically active products. To date, research has been focussed primarily on Old Yellow Enzyme-like proteins, due to their ease of handling, whe... Read More about Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes.

An automated fabrication strategy to create patterned tubular architectures at cell and tissue scales (2015)
Journal Article

Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation (2014)
Journal Article
Dixon, J. E., Shah, D. A., Rogers, C., Hall, S., Weston, N., Parmenter, C. D., …Shakesheff, K. M. (2014). Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. Proceedings of the National Academy of Sciences, 111(15), 5580-5585. https://doi.org/10.1073/pnas.1319685111

The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the... Read More about Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation.