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Thecal cell sensitivity to luteinizing hormone and insulin in polycystic ovarian syndrome

Cadagan, David; Khan, Raheela; Amer, Saad A.

Authors

David Cadagan

RAHEELA KHAN RAHEELA.KHAN@NOTTINGHAM.AC.UK
Professor of Cellular Physiology

Profile image of SAAD AMER

SAAD AMER saad.amer@nottingham.ac.uk
Professor of Gynaecology and Reproductive Medicine



Abstract

This study examined whether a defect of steroid synthesis in ovarian theca cells may lead to the development of PCOS, through contributions to excess androgen secretion.

Polycystic ovarian syndrome (PCOS) is one of the leading causes of infertility worldwide affecting around 1 in 10 of women of a reproductive age. One of the fundamental abnormalities in this syndrome is the presence of hormonal irregularities, including hyperandrogenemia, hyperinsulinemia and hypersecretion of luteinizing hormone (LH). Studies suggest that insulin treatment increases progesterone and androstenedione secretion in PCOS theca cells when compared to insulin treated normal theca cells. Furthermore the augmented effects of LH and insulin have been seen to increase ovarian androgen synthesis in non-PCOS theca cultures whilst also increasing the expression of steroidogenic enzymes specific to the PI3-K pathway.

Our examination of primary thecal cultures showed an increase in both the expression of the steroidogenic enzyme CYP17 and androgen secretion in PCOS theca cells under basal conditions, when compared to non-PCOS cells. This was increased significantly under treatments of LH and insulin combined.

Our results support the previous reported hypothesis that a dysfunction may exist within the PI3-K pathway. Specifically, that sensitivity exists to physiological symptoms including hyperinsulinemia and hyper secretion of LH found in PCOS through co-stimulation. The impact of these findings may allow the development of a therapeutic target in PCOS.

Citation

Cadagan, D., Khan, R., & Amer, S. A. (2016). Thecal cell sensitivity to luteinizing hormone and insulin in polycystic ovarian syndrome. Reproductive Biology -Olsztyn-, 16(1), https://doi.org/10.1016/j.repbio.2015.12.006

Journal Article Type Article
Acceptance Date Dec 30, 2015
Online Publication Date Jan 13, 2016
Publication Date Mar 31, 2016
Deposit Date Jul 17, 2017
Journal Reproductive Biology
Electronic ISSN 2300-732X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 16
Issue 1
DOI https://doi.org/10.1016/j.repbio.2015.12.006
Keywords 17α-Hydroxylase, Androgen, Insulin, Luteinizing hormone, Theca
Public URL https://nottingham-repository.worktribe.com/output/778281
Publisher URL https://doi.org/10.1016/j.repbio.2015.12.006
Contract Date Jul 17, 2017