Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels

Sander, Katrin N.; Hay, Daniel P.; Broughton-Pipkin, Fiona; Khan, Raheela N.; Barrett, David A.

Abstract

© 2018 The analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect on the quality of quantitative results. Therefore, a range of mechanical homogenisation techniques and normalisation strategies were evaluated for application to human placental vessels. The findings showed that rotor-stator homogenisation in a suitable solvent and wet weight normalisation were the best combination of procedures for quantitative analysis of lipids in placental blood vessels.

Citation

Sander, K. N., Hay, D. P., Broughton-Pipkin, F., Khan, R. N., & Barrett, D. A. (2018). Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels. Journal of Pharmaceutical and Biomedical Analysis, 160, 297-300. https://doi.org/10.1016/j.jpba.2018.08.012

• Journal Article Type: Article
• Acceptance Date: Aug 6, 2018
• Online Publication Date: Aug 7, 2018
• Publication Date: Oct 25, 2018
• Deposit Date: Aug 8, 2018
• Publicly Available Date: Aug 8, 2019
• Journal: Journal of Pharmaceutical and Biomedical Analysis
• Print ISSN: 0731-7085
• Electronic ISSN: 1873-264X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 160
• Pages: 297-300
• DOI: https://doi.org/10.1016/j.jpba.2018.08.012
• Keywords: Eicosanoids; Oxylipins
• Public URL: https://nottingham-repository.worktribe.com/output/973519
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0731708518311373?via%3Dihub
• Contract Date: Aug 8, 2018