Noha M. Abouseada
Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2
Abouseada, Noha M.; Assafi, Mahde Saleh A.; Mahdavi, Jafar; Oldfield, Neil J.; Wheldon, Lee M.; Wooldridge, Karl G.; Ala'Aldeen, Dlawer A.A.
Authors
Mahde Saleh A. Assafi
Jafar Mahdavi
Neil J. Oldfield
Lee M. Wheldon
Karl G. Wooldridge
Dlawer A.A. Ala'Aldeen
Abstract
Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae are major bacterial agents of meningitis. They each bind the 37/67-kDa laminin receptor (LamR) via the surface protein adhesins: meningococcal PilQ and PorA, H. influenzae OmpP2 and pneumococcal CbpA. We have previously reported that a surface-exposed loop of the R2 domain of CbpA mediates LamR-binding. Here we have identified the LamR-binding regions of PorA and OmpP2. Using truncated recombinant proteins we show that binding is dependent on amino acids 171–240 and 91–99 of PorA and OmpP2, respectively, which are predicted to localize to the fourth and second surface-exposed loops, respectively, of these proteins. Synthetic peptides corresponding to the loops bound LamR and could block LamR-binding to bacterial ligands in a dose dependant manner. Meningococci expressing PorA lacking the apex of loop 4 and H. influenzae expressing OmpP2 lacking the apex of loop 2 showed significantly reduced LamR binding. Since both loops are hyper-variable, our data may suggest a molecular basis for the range of LamR-binding capabilities previously reported among different meningococcal and H. influenzae strains.
Citation
Abouseada, N. M., Assafi, M. S. A., Mahdavi, J., Oldfield, N. J., Wheldon, L. M., Wooldridge, K. G., & Ala'Aldeen, D. A. (2012). Mapping the laminin receptor binding domains of Neisseria meningitidis PorA and Haemophilus influenzae OmpP2. PLoS ONE, 7(9), Article 10. https://doi.org/10.1371/journal.pone.0046233
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 28, 2012 |
Publication Date | Sep 25, 2012 |
Deposit Date | Apr 14, 2014 |
Publicly Available Date | Apr 14, 2014 |
Journal | PLoS ONE |
Electronic ISSN | 1932-6203 |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 9 |
Article Number | 10 |
DOI | https://doi.org/10.1371/journal.pone.0046233 |
Public URL | https://nottingham-repository.worktribe.com/output/711247 |
Publisher URL | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0046233 |
Contract Date | Apr 14, 2014 |
Files
journal.pone.0046233.pdf
(565 Kb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
You might also like
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search