Measurement of fasted state gastric antral motility before and after a standard bioavailability and bioequivalence 240 mL drink of water: Validation of MRI method against concomitant perfused manometry in healthy participants

Heissam, Khaled; Abrehart, Nichola; Hoad, Caroline L.; Wright, Jeff; Menys, Alex; Murray, Kathryn; Glover, Paul M.; Hebbard, Geoffrey; Gowland, Penny A.; Baker, Jason; Hasler, William L.; Spiller, Robin C.; Corsetti, Maura; Brasseur, James G.; Hens, Bart; Shedden, Kerby; Dickens, Joseph; Mudie, Deanna M.; Amidon, Greg E.; Amidon, Gordon L.; Marciani, Luca

Abstract

Objective The gastrointestinal environment in which drug products need to disintegrate before the drug can dissolve and be absorbed has not been studied in detail due to limitations, especially invasiveness of existing techniques. Minimal in vivo data is available on undisturbed gastrointestinal motility to improve relevance of predictive dissolution models and in silico tools such as physiologically-based pharmacokinetic models. Recent advances in magnetic resonance imaging methods could provide novel data and insights that can be used as a reference to validate and, if necessary, optimize these models. The conventional method for measuring gastrointestinal motility is via a manometric technique involving intubation. Nevertheless, it is feasible to measure gastrointestinal motility with magnetic resonance imaging. The aim of this study was is to develop and validate a magnetic resonance imaging method using the most recent semi-automated analysis method against concomitant perfused manometry method. Material and methods Eighteen healthy fasted participants were recruited for this study. The participants were intubated with a water-perfused manometry catheter. Subsequently, stomach motility was assessed by cine-MRI acquired at intervals, of 3.5min sets, at coronal oblique planes through the abdomen and by simultaneous water perfused manometry, before and after administration of a standard bioavailability / bioequivalence 8 ounces (~240mL) drink of water. The magnetic resonance imaging motility images were analysed using Spatio-Temporal Motility analysis STMM techniques. The area under the curve of the gastric motility contractions was calculated for each set and compared between techniques. The study visit was then repeated one week later. Results Data from 15 participants was analysed. There was a good correlation between the MRI antral motility plots area under the curve and corresponding perfused manometry motility area under the curve (r = 0.860) during both antral contractions and quiescence. Conclusion Non-invasive dynamic magnetic resonance imaging of gastric antral motility coupled with recently developed, semi-automated magnetic resonance imaging data processing techniques correlated well with simultaneous, ‘gold standard’ water perfused manometry. This will be particularly helpful for research purposes related to oral absorption where the absorption of a drug is highly depending on the underlying gastrointestinal processes such as gastric emptying, gastrointestinal motility and availability of residual fluid volumes. Clinical trial This trial was registered at ClinicalTrials.gov as NCT03191045.

Citation

Heissam, K., Abrehart, N., Hoad, C. L., Wright, J., Menys, A., Murray, K., …Marciani, L. (2020). Measurement of fasted state gastric antral motility before and after a standard bioavailability and bioequivalence 240 mL drink of water: Validation of MRI method against concomitant perfused manometry in healthy participants. PLoS ONE, 15(11), e0241441. https://doi.org/10.1371/journal.pone.0241441

• Journal Article Type: Article
• Acceptance Date: Oct 21, 2020
• Online Publication Date: Nov 11, 2020
• Publication Date: Nov 11, 2020
• Deposit Date: Oct 23, 2020
• Publicly Available Date: Nov 11, 2020
• Journal: PLOS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 11
• Pages: e0241441
• DOI: https://doi.org/10.1371/journal.pone.0241441
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/4984899
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241441