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Serum metabolomic alterations in Beagle dogs experimentally infected with Toxocara canis

Zou, Yang; Zheng, Wen-Bin; Elsheikha, Hany M.; Liu, Guo-Hua; Hu, Min-Hua; Wang, Shui-Lian; Zhu, Xing-Quan

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Authors

Yang Zou

Wen-Bin Zheng

Guo-Hua Liu

Min-Hua Hu

Shui-Lian Wang

Xing-Quan Zhu



Abstract

Background
Toxocara canis, a globally distributed roundworm, can cause debilitating disease in dogs and humans; however, little is known about the metabolomic response of the hosts to T. canis infection. There is an increasing need to understand the metabolic mechanisms underlying the pathogenesis of T. canis infection in dogs. Here, we examined the metabolomic changes in Beagle dogsʼ serum following T. canis infection using LC-MS/MS.

Results
The metabolic profiles of Beagle dogsʼ serum were determined at 12 h, 24 h, 10 d and 36 d after oral infection with 300 infectious T. canis eggs by LC-MS/MS. We tested whether the T. canis-associated differentially abundant metabolites could distinguish the serum of infected dogs from controls, as measured by the area under the receiver operating characteristic (ROC) curve (AUC). The differentially expressed metabolites were further evaluated by principal components analysis and pathway enrichment analysis. A total of 5756 and 5299 ions were detected in ESI+ and ESI− mode, respectively. ROC curve analysis revealed nine and five metabolite markers, at 12 hpi and 24 hpi to 36 dpi, respectively, with potential diagnostic value for toxocariasis. The levels of taurocholate, estradiol, prostaglandins and leukotriene were significantly changed. Primary bile acid biosynthesis pathway, steroid hormone biosynthesis pathway and biosynthesis of unsaturated fatty acids pathway were significantly altered by T. canis infection.

Conclusions
These findings show that T. canis infection can induce several changes in the dog serum metabolome and that the metabolic signature associated with T. canis infection in dogs has potential for toxocariasis diagnosis.

Citation

Zou, Y., Zheng, W.-B., Elsheikha, H. M., Liu, G.-H., Hu, M.-H., Wang, S.-L., & Zhu, X.-Q. (2019). Serum metabolomic alterations in Beagle dogs experimentally infected with Toxocara canis. Parasites and Vectors, 12(1), 1-10. https://doi.org/10.1186/s13071-019-3703-5

Journal Article Type Article
Acceptance Date Sep 4, 2019
Online Publication Date Sep 11, 2019
Publication Date 2019-12
Deposit Date Sep 17, 2019
Publicly Available Date Sep 17, 2019
Journal Parasites & Vectors
Electronic ISSN 1756-3305
Publisher Springer Nature
Peer Reviewed Peer Reviewed
Volume 12
Issue 1
Article Number 447
Pages 1-10
DOI https://doi.org/10.1186/s13071-019-3703-5
Keywords Toxocara canis; Toxocariasis; Beagle dog; Serum; Metabolomic; LC-MS/MS
Public URL https://nottingham-repository.worktribe.com/output/2625053
Publisher URL https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-019-3703-5
Contract Date Sep 17, 2019

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