Nigel De Melo
Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells
De Melo, Nigel; McGinlay, Sarah; Markus, Robert; Macri-Pellizzeri, Laura; Symonds, Michael E.; Ahmed, Ifty; Sottile, Virginie
Authors
Sarah McGinlay
Robert Markus
LAURA MACRI PELLIZZERI Laura.Macripellizzeri@nottingham.ac.uk
Building Team Senior Technician
Michael E. Symonds
Associate Professor IFTY AHMED ifty.ahmed@nottingham.ac.uk
Professor of Materials Science and Engineering
Virginie Sottile
Abstract
Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existing in bone marrow. When isolated in vitro, these stem cells recapitulate osteoblast or adipocyte formation if treated with specialised media, modelling how these lineages interact in vivo. Osteogenic differentiation is characterised by mineral deposits accumulating in the extracellular matrix, typically assessed using histological techniques. Adipogenesis occurs with accumulation of intracellular lipids that can be routinely visualised by Oil Red O staining. In both cases, staining requires cell fixation and is thus limited to end-point assessments. Here, a vital staining approach was developed to simultaneously detect mineral deposits and lipid droplets in differentiating cultures. Stem cells induced to differentiate produced mixed cultures containing adipocytes and bone-like nodules, and after two weeks live cultures were incubated with tetracycline hydrochloride and Bodipy to label mineral- and lipid-containing structures, respectively. Fluorescence microscopy showed the simultaneous visualisation of mineralised areas and lipid-filled adipocytes in live cultures. Combined with the nuclear stain Hoechst 33258, this approach further enabled live confocal imaging of adipogenic cells interspersed within the mineralised matrix. This multiplex labelling was repeated at subsequent time-points, demonstrating the potential of this new approach for the real-time high-precision imaging of live stem cells.
Citation
De Melo, N., McGinlay, S., Markus, R., Macri-Pellizzeri, L., Symonds, M. E., Ahmed, I., & Sottile, V. (2019). Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics, 4(3), 1-10. https://doi.org/10.3390/biomimetics4030048
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 4, 2019 |
Online Publication Date | Jul 10, 2019 |
Publication Date | Jul 10, 2019 |
Deposit Date | Jul 12, 2019 |
Publicly Available Date | Jul 12, 2019 |
Journal | Biomimetics |
Print ISSN | 2313-7673 |
Electronic ISSN | 2313-7673 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 4 |
Issue | 3 |
Article Number | 48 |
Pages | 1-10 |
DOI | https://doi.org/10.3390/biomimetics4030048 |
Keywords | live monitoring; stem cell; mineralisation; lipid detection |
Public URL | https://nottingham-repository.worktribe.com/output/2302295 |
Publisher URL | https://www.mdpi.com/2313-7673/4/3/48 |
Contract Date | Jul 12, 2019 |
Files
Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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