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In situ monitoring of galactolipid digestion by infrared spectroscopy in both model micelles and spinach chloroplasts (2023)
Journal Article
Sahaka, M., Mateos-Diaz, E., Amara, S., Wattanakul, J., Gray, D., Lafont, D., …Carrière, F. (2023). In situ monitoring of galactolipid digestion by infrared spectroscopy in both model micelles and spinach chloroplasts. Chemistry and Physics of Lipids, 252, 105291. https://doi.org/10.1016/j.chemphyslip.2023.105291

Galactolipids are the main lipids from plant photosynthetic membranes and they can be digested by pancreatic lipase related protein 2 (PLRP2), an enzyme found in the pancreatic secretion in many animal species. Here, we used transmission Fourier-tran... Read More about In situ monitoring of galactolipid digestion by infrared spectroscopy in both model micelles and spinach chloroplasts.

Chloroplast/thylakoid-rich material: A possible alternative to the chemically synthesised flow enhancer polyglycerol polyricinoleate in oil-based systems (2023)
Journal Article
Sutcharit, P., Wattanakul, J., Price, R., Di Bari, V., Gould, J., Yakubov, G., …Gray, D. A. (2023). Chloroplast/thylakoid-rich material: A possible alternative to the chemically synthesised flow enhancer polyglycerol polyricinoleate in oil-based systems. Food Research International, 165, Article 112472. https://doi.org/10.1016/j.foodres.2023.112472

Chloroplasts are abundant organelles in a diverse range of plant materials; they are predominantly composed of multicomponent thylakoid membranes which are lipid and protein rich. Intact or unravelled thylakoid membranes should, in principle, have in... Read More about Chloroplast/thylakoid-rich material: A possible alternative to the chemically synthesised flow enhancer polyglycerol polyricinoleate in oil-based systems.

Linking the yield stress functionality of polyglycerol polyricinoleate in a highly filled suspension to its molecular properties (2022)
Journal Article
Price, R., Gray, D., Watson, N., Vieira, J., & Wolf, B. (2022). Linking the yield stress functionality of polyglycerol polyricinoleate in a highly filled suspension to its molecular properties. LWT - Food Science and Technology, 165, Article 113704. https://doi.org/10.1016/j.lwt.2022.113704

Polyglycerol polyricinoleate (PGPR) is a food emulsifier with a unique yield stress reducing efficacy in fat-based suspensions. There are many commercially available PGPRs, and the different products vary in their impact on the yield stress. Choosing... Read More about Linking the yield stress functionality of polyglycerol polyricinoleate in a highly filled suspension to its molecular properties.

Is oral lipid-based delivery for drug targeting to the brain feasible? (2022)
Journal Article
Brookes, A., Ji, L., Bradshaw, T. D., Stocks, M., Gray, D., Butler, J., & Gershkovich, P. (2022). Is oral lipid-based delivery for drug targeting to the brain feasible?. European Journal of Pharmaceutics and Biopharmaceutics, 172, 112-122. https://doi.org/10.1016/j.ejpb.2022.02.004

This review outlines the feasibility of oral lipid-based targeted delivery of drugs to the brain, including permeation of the central nervous system's (CNS) protective blood–brain barrier (BBB). The structure of the BBB and disruption caused by varyi... Read More about Is oral lipid-based delivery for drug targeting to the brain feasible?.

A mathematical model of a single seed oleosome (2021)
Journal Article
Meacci, L., di Bari, V., Ausas, R. F., Mut, F., Gray, D. A., & Buscaglia, G. C. (2021). A mathematical model of a single seed oleosome. Applied Mathematics, 9, Article 100128. https://doi.org/10.1016/j.rinam.2020.100128

© 2020 The Authors In this work we report for the first time a mathematical approach to model the behaviour of a single oleosome (oil body) within a seed cellular environment. To describe the behaviour of the oleosome membrane, we adopted a dynamical... Read More about A mathematical model of a single seed oleosome.

Food biotechnology (2020)
Journal Article
Foster, T., Adams, G., di Bari, V., Connerton, I., Gould, J., Gouseti, O., …Yakubov, G. (2020). Food biotechnology. Current Opinion in Chemical Engineering, 30, 53-59. https://doi.org/10.1016/j.coche.2020.08.006

We investigate recent advances in the Chemical Engineering aspects of food structuring agents and macronutrients: carbohydrates, proteins and lipids, and also the fate of food upon ingestion. Prebiotic effects on host-microbe interactions enable impr... Read More about Food biotechnology.

Spinach leaf and chloroplast lipid: A natural rheology modifier for chocolate? (2020)
Journal Article
Mohamad, N. J., Gray, D., & Wolf, B. (2020). Spinach leaf and chloroplast lipid: A natural rheology modifier for chocolate?. Food Research International, 133, Article 109193. https://doi.org/10.1016/j.foodres.2020.109193

© 2020 Elsevier Ltd In this study the possibility of replacing current surfactants in chocolate formulations with natural lipids extracted from spinach leaf (SPLIP) or spinach chloroplast (CH.SPLIP) was evaluated. SPLIP and CH.SPLIP were extracted wi... Read More about Spinach leaf and chloroplast lipid: A natural rheology modifier for chocolate?.

Assessment of rapeseed oil body (oleosome) lipolytic activity as an effective predictor of emulsion purity and stability (2020)
Journal Article
De Chirico, S., di Bari, V., Romero Guzmán, M. J., Nikiforidis, C., Foster, T., & Gray, D. (2020). Assessment of rapeseed oil body (oleosome) lipolytic activity as an effective predictor of emulsion purity and stability. Food Chemistry, 316, Article 126355. https://doi.org/10.1016/j.foodchem.2020.126355

© 2020 Elsevier Ltd The lipolytic activity in oil body creams as affected by recovery and washing protocols was investigated. The effect of thermal treatment on the hydrolytic activity and physical stability of fresh and aged (up to 30 days) oil body... Read More about Assessment of rapeseed oil body (oleosome) lipolytic activity as an effective predictor of emulsion purity and stability.

In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest pea vine field residue (halum) and spinach leaves (2019)
Journal Article
Wattanakul, J., Sahaka, M., Amara, S., Mansor, S., Gontero, B., Carrière, F., & Gray, D. (2019). In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest pea vine field residue (halum) and spinach leaves. Food and Function, 10(12), 7806-7817. https://doi.org/10.1039/C9FO01867K

The removal of intact chloroplasts from their cell wall confinement offers a novel way to obtain lipophilic nutrients from green biomass, especially carotenoids and galactolipids. These latter are the main membrane lipids in plants and they represent... Read More about In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest pea vine field residue (halum) and spinach leaves.

The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7 (2019)
Journal Article
Juliana Romero-Guzmán, M., Petris, V., De Chirico, S., di Bari, V., Gray, D., Boom, R. M., & Nikiforidis, C. V. (2020). The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7. Food Chemistry, 306, Article 125578. https://doi.org/10.1016/j.foodchem.2019.125578

Oleosomes are storage vehicles of TAGs in plant seeds. They are protected with a phospholipid-protein monolayer and extracted with alkaline aqueous media; however, pH adjustment intensifies the extraction process. Therefore the aim of this work was t... Read More about The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7.