The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7

Juliana Romero-Guzm�n, Maria; Petris, Vasileios; De Chirico, Simone; di Bari, Vincenzo; Gray, David; Boom, Remko M.; Nikiforidis, Constantinos V.

Abstract

Oleosomes are storage vehicles of TAGs in plant seeds. They are protected with a phospholipid-protein monolayer and extracted with alkaline aqueous media; however, pH adjustment intensifies the extraction process. Therefore the aim of this work was to investigate the extraction mechanism of rapeseed oleosomes at pH 7 and at the presence of monovalent and divalent cations (Na+, K+, Mg2+, and Ca+2). The oleosome yield at pH 9.5 was 64 wt%, while the yield at pH 7 with H2O was just 43 wt%. The presence of cations at pH 7, significantly enhanced the yield, with K+ giving the highest yield (64 wt%). The cations affected the oleosome interface and their interactions. The presence of monovalent cations resulted in aggregation and minor coalescence, while divalent cations resulted in extensive coalescence. These results help to understand the interactions of oleosomes in their native matrix and design simple extraction processes at neutral conditions.

Citation

Juliana Romero-Guzmán, M., Petris, V., De Chirico, S., di Bari, V., Gray, D., Boom, R. M., & Nikiforidis, C. V. (2020). The effect of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations on rapeseed oleosome (oil body) extraction and stability at pH 7. Food Chemistry, 306, Article 125578. https://doi.org/10.1016/j.foodchem.2019.125578

• Journal Article Type: Article
• Acceptance Date: Sep 22, 2019
• Online Publication Date: Oct 4, 2019
• Publication Date: Feb 15, 2020
• Deposit Date: Oct 11, 2019
• Publicly Available Date: Oct 5, 2020
• Journal: Food Chemistry
• Print ISSN: 0308-8146
• Electronic ISSN: 1873-7072
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 306
• Article Number: 125578
• DOI: https://doi.org/10.1016/j.foodchem.2019.125578
• Keywords: Oil bodies; Extraction; Natural emulsion; Rapeseed; Oleosomes
• Public URL: https://nottingham-repository.worktribe.com/output/2803442
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0308814619317029?via%3Dihub
• Contract Date: Oct 11, 2019