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NICK HANNAN's Outputs (18)

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications (2024)
Journal Article
Durczak, P. M., Fair, K. L., Jinks, N., Cuevas Ocaña, S., Sainz Zuñiga, C. B., & Hannan, N. R. F. (2024). Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications. Journal of Visualized Experiments, 2024(205), Article e61199. https://doi.org/10.3791/61199

hiPSC-derived intestinal organoids are epithelial structures that self-assemble from differentiated cells into complex 3D structures, representative of the human intestinal epithelium, in which they exhibit crypt/villus-like structures. Here, we desc... Read More about Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications.

A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells (2023)
Journal Article
Cuevas-Ocaña, S., Yang, J. Y., Aushev, M., Schlossmacher, G., Bear, C. E., Hannan, N. R., …Gray, M. A. (2023). A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells. International Journal of Molecular Sciences, 24(12), Article 10266. https://doi.org/10.3390/ijms241210266

Introducing or correcting disease-causing mutations through genome editing in human pluripotent stem cells (hPSCs) followed by tissue-specific differentiation provide sustainable models of multiorgan diseases, such as cystic fibrosis (CF). However, l... Read More about A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells.

Identification and characterisation of a rare MTTP variant underlying hereditary non-alcoholic fatty liver disease (2023)
Journal Article
Grove, J. I., Lo, P. C., Shrine, N., Barwell, J., Wain, L. V., Tobin, M. D., …Aithal, G. P. (2023). Identification and characterisation of a rare MTTP variant underlying hereditary non-alcoholic fatty liver disease. JHEP Reports, 5(8), Article 100764. https://doi.org/10.1016/j.jhepr.2023.100764

Background & Aims: Non-alcoholic fatty liver disease (NAFLD) is a complex trait with an estimated prevalence of 25% globally. We aimed to identify the genetic variant underlying a four-generation family with progressive NAFLD leading to cirrhosis, de... Read More about Identification and characterisation of a rare MTTP variant underlying hereditary non-alcoholic fatty liver disease.

Regional differences in human biliary tissues and corresponding in vitro derived organoids (2020)
Journal Article
Rimland, C. A., Tilson, S. G., Morell, C. M., Tomaz, R. A., Lu, W.-Y., Adams, S. E., …Vallier, L. (2021). Regional differences in human biliary tissues and corresponding in vitro derived organoids. Hepatology, 73(1), 247-267. https://doi.org/10.1002/hep.31252

Background
Organoids provide a powerful system to study epithelia in vitro . Recently, this approach was applied successfully to the biliary tree, a series of ductular tissues responsible for the drainage of bile and pancreatic secretions. More prec... Read More about Regional differences in human biliary tissues and corresponding in vitro derived organoids.

hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency (2018)
Journal Article
Segeritz, C., Rashid, S. T., de Brito, M. C., Serra, M. P., Ordonez, A., Morell, C. M., …Vallier, L. (2018). hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency. Journal of Hepatology, 69(4), 851-860. https://doi.org/10.1016/j.jhep.2018.05.028

© 2018 The Authors Background & Aims: α1-Antitrypsin deficiency (A1ATD) is an autosomal recessive disorder caused by mutations in the SERPINA1 gene. Individuals with the Z variant (Gly342Lys) retain polymerised protein in the endoplasmic reticulum (E... Read More about hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency.

Intestinal organoids for modelling intestinal development and disease (2018)
Journal Article
Fair, K. L., Colquhoun, J., & Hannan, N. R. F. (2018). Intestinal organoids for modelling intestinal development and disease. Philosophical Transactions B: Biological Sciences, 373(1750), Article 20170217. https://doi.org/10.1098/rstb.2017.0217

Gastrointestinal diseases are becoming increasingly prevalent in developed countries. Immortalized cells and animal models have delivered important but limited insight into the mechanisms that initiate and propagate these diseases. Human-specific mod... Read More about Intestinal organoids for modelling intestinal development and disease.

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids (2017)
Journal Article
Sampaziotis, F., Justin, A. W., Tysoe, O. C., Sawiak, S., Godfrey, E. M., Upponi, S. S., …Vallier, L. (2017). Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Nature Medicine, 23(8), 954-963. https://doi.org/10.1038/nm.4360

© 2017 Nature America, Inc., part of Springer Nature. All rights reserved. The treatment of common bile duct (CBD) disorders, such as biliary atresia or ischemic strictures, is restricted by the lack of biliary tissue from healthy donors suitable for... Read More about Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids.

Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells (2017)
Journal Article
Sampaziotis, F., de Brito, M. C., Geti, I., Bertero, A., Hannan, N. R., & Vallier, L. (2017). Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Nature Protocols, 12(4), 814-827. https://doi.org/10.1038/nprot.2017.011

The difficulty in isolating and propagating functional primary cholangiocytes is a major limitation in the study of biliary disorders and the testing of novel therapeutic agents. To overcome this problem, we have developed a platform for the differen... Read More about Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells.

Dynamics of 5-carboxylcytosine during hepatic differentiation: potential general role for active demethylation by DNA repair in lineage specification (2017)
Journal Article
Lewis, L. C., Lo, P. C. K., Foster, J. M., Dai, N., Correa, I. R., Durczak, P. M., …Ruzov, A. (in press). Dynamics of 5-carboxylcytosine during hepatic differentiation: potential general role for active demethylation by DNA repair in lineage specification. Epigenetics, 12(4), https://doi.org/10.1080/15592294.2017.1292189

Patterns of DNA methylation (5-methylcytosine, 5mC) are rearranged during differentiation contributing to the regulation of cell type-specific gene expression. TET proteins oxidise 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-c... Read More about Dynamics of 5-carboxylcytosine during hepatic differentiation: potential general role for active demethylation by DNA repair in lineage specification.

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs (2016)
Journal Article
Bertero, A., Pawlowski, M., Ortmann, D., Snijders, K., Yiangou, L., Cardoso de Brito, M., …Vallier, L. (2016). Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Development, 143(23), 4405-4418. https://doi.org/10.1242/dev.138081

© 2016. Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address thes... Read More about Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation (2015)
Journal Article
Sampaziotis, F., Cardoso de Brito, M., Madrigal, P., Bertero, A., Saeb-Parsy, K., Soares, F. A., …Vallier, L. (in press). Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature Biotechnology, 33(8), https://doi.org/10.1038/nbt.3275

The study of biliary disease has been constrained by a lack of primary human cholangiocytes. Here we present an efficient, serum-free protocol for directed differentiation of human induced pluripotent stem cells into cholangiocyte-like cells (CLCs).... Read More about Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation.

Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture (2014)
Journal Article
Gieseck, R. L., Hannan, N. R., Gieseck III, R., Bort, R., Hanley, N., Drake, R., …Vallier, L. (2014). Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture. PLoS ONE, 9(1), Article e86372. https://doi.org/10.1371/journal.pone.0086372

Induced pluripotent stem cell derived hepatocytes (IPSC-Heps) have the potential to reduce the demand for a dwindling number of primary cells used in applications ranging from therapeutic cell infusions to in vitro toxicology studies. However, curren... Read More about Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture.