Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris
(2011)
Journal Article
Whyteside, G., Alcocer, M. J., Kumita, J. R., Dobson, C. M., Lazarou, M., Pleass, R. J., & Archer, D. B. (2011). Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris. PLoS ONE, 6(7), Article e22692. https://doi.org/10.1371/journal.pone.0022692
We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response (UPR), ER-associated d... Read More about Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.