All Outputs (133)

Enhanced uptake in 2D- and 3D- lung cancer cell models of redox responsive PEGylated nanoparticles with sensitivity to reducing extra-and intracellular environments (2018)
Journal Article
Conte, C., Mastrotto, F., Taresco, V., Tchoryk, A., Quaglia, F., Stolnik, S., & Alexander, C. (2018). Enhanced uptake in 2D- and 3D- lung cancer cell models of redox responsive PEGylated nanoparticles with sensitivity to reducing extra-and intracellular environments. Journal of Controlled Release, 277, 126-141. https://doi.org/10.1016/j.jconrel.2018.03.011

In the treatment of lung cancer, there is an urgent need of innovative medicines to optimize pharmacological responses of conventional chemotherapeutics while attenuating side effects. Here, we have exploited some relatively unexplored subtle differe... Read More about Enhanced uptake in 2D- and 3D- lung cancer cell models of redox responsive PEGylated nanoparticles with sensitivity to reducing extra-and intracellular environments.

Comparison of gene transfection and cytotoxicity mechanisms of linear poly(amidoamine) and branched poly(ethyleneimine) polyplexes (2018)
Journal Article
Almulathanon, A. A., Ranucci, E., Ferruti, P., Garnett, M. C., & Bosquillon, C. (2018). Comparison of gene transfection and cytotoxicity mechanisms of linear poly(amidoamine) and branched poly(ethyleneimine) polyplexes. Pharmaceutical Research, 35(4), Article 86. https://doi.org/10.1007/s11095-017-2328-7

Purpose: This study aimed to further explore the mechanisms behind the ability of certain linear polyamidoamines (PAAs) to transfect cells with minimal cytotoxicity.
Methods: The transfection efficiency of DNA complexed with a PAA of a molecular we... Read More about Comparison of gene transfection and cytotoxicity mechanisms of linear poly(amidoamine) and branched poly(ethyleneimine) polyplexes.

Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands (2018)
Journal Article
Alea-Reyes, M. E., Penon, O., García Calavia, P., Marín, M. J., Russell, D. A., & Pérez-García, L. (2018). Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands. Journal of Colloid and Interface Science, 521, https://doi.org/10.1016/j.jcis.2018.03.014

Hypothesis

Metalloporphyrins are extensively investigated for their ability to form reactive oxygen species and as potent photosensitisers for use in photodynamic therapy. However, their hydrophobicity generally causes solubility issues concerning... Read More about Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands.

Self-assembling benzothiazole-based gelators: a mechanistic understanding of in vitro bioactivation and gelation (2018)
Journal Article
Citossi, F., Smith, T., Lee, J. B., Segal, J., Gershkovich, P., Stocks, M. J., Bradshaw, T. D., Kellam, B., & Marlow, M. (in press). Self-assembling benzothiazole-based gelators: a mechanistic understanding of in vitro bioactivation and gelation. Molecular Pharmaceutics, 15(4), https://doi.org/10.1021/acs.molpharmaceut.7b01106

Low molecular weight gelators (LMWGs) of chemotherapeutic drugs represent a valid alternative to the existing poly-mer-based formulations used for targeted delivery of anticancer drugs. Herein we report the design and development of novel self-assemb... Read More about Self-assembling benzothiazole-based gelators: a mechanistic understanding of in vitro bioactivation and gelation.

“Fever means antibiotic”, the Omani public’s attitudes to the use of antibiotics for treating the common cold (2018)
Journal Article
Al Juma, M. A., Anderson, C., & Boyd, M. J. (2018). “Fever means antibiotic”, the Omani public’s attitudes to the use of antibiotics for treating the common cold

Background:
Antimicrobial resistance is a worldwide concern to reserve the effectiveness of antibiotics in therapy. The irrational use of antibiotics is one of the factors contributing to antimicrobial resistance. In depth exploration of the public... Read More about “Fever means antibiotic”, the Omani public’s attitudes to the use of antibiotics for treating the common cold.

Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA (2018)
Journal Article
Capel, V., Vllasaliu, D., Watts, P., Clarke, P. A., Luxton, D., Grabowska, A. M., Mantovani, G., & Stolnik, S. (in press). Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA. Drug Delivery, 25(1), https://doi.org/10.1080/10717544.2018.1440668

Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform... Read More about Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.

Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells (2018)
Journal Article
Alshehri, A. A., Grabowska, A. M., & Stolnik, S. (in press). Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-22166-3

Design of an efficient delivery system is a generally recognised bottleneck in translation of siRNA technology into clinic. Despite research efforts, cellular processes that determine efficiency of siRNA silencing achieved by different delivery formu... Read More about Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells.

Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles (2018)
Journal Article
Sayers, E., Magnusson, J. P., Moody, P., Mastrotto, F., Conte, C., Brazzale, C., Borri, P., Caliceti, P., Watson, P., Mantovani, G., Aylott, J., Salmaso, S., Jones, A. T., & Alexander, C. (2018). Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles. Bioconjugate Chemistry, 29(4), 1030-1046. https://doi.org/10.1021/acs.bioconjchem.7b00704

© 2018 American Chemical Society. Ligand-mediated targeting and internalization of plasma membrane receptors is central to cellular function. These types of receptors have accordingly been investigated as targets to facilitate entry of diagnostic and... Read More about Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles.

A biomaterials approach to influence stem cell fate in injectable cell-based therapies (2018)
Journal Article
Amer, M. H., Rose, F. R., Shakesheff, K. M., & White, L. J. (2018). A biomaterials approach to influence stem cell fate in injectable cell-based therapies. Stem Cell Research and Therapy, 9(39), https://doi.org/10.1186/s13287-018-0789-1

Background: Numerous stem cell therapies use injection-based administration to deliver high density cell preparations. However, cell retention rates as low as 1% have been observed within days of transplantation. This study investigated the effects o... Read More about A biomaterials approach to influence stem cell fate in injectable cell-based therapies.

Drug delivery across length scales (2018)
Journal Article
Delcassian, D., Patel, A. K., Cortinas, A. B., & Langer, R. (2019). Drug delivery across length scales. Journal of Drug Targeting, 27(3), 229-243. https://doi.org/10.1080/1061186X.2018.1438440

Over the last century, there has been a dramatic change in the nature of therapeutic, biologically active molecules available to treat disease. Therapies have evolved from extracted natural products towards rationally designed biomolecules, including... Read More about Drug delivery across length scales.

Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege (2018)
Journal Article
Hassan, A., Pritchard, D. I., & Ghaemmaghami, A. M. (2018). Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege. Parasitology, 145(9), 1183-1190. https://doi.org/10.1017/S0031182018000136

Despite the profound health implications of Necator americanus (N. americanus) infection in humans, many aspects of its interaction with the host immune system are poorly understood. Here we investigated the early events at the interface of N. americ... Read More about Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege.

Hydrodynamically Guided Hierarchical Self-Assembly of Peptide-Protein Bioinks (2018)
Journal Article
Ng, K. W., Hedegaard, C. L., Collin, E. C., Castrejón-Pita, A. A., Redondo-Gómez, C., Nguyen, L. T. H., Castrejón-Pita, J. R., & Mata, A. (2018). Hydrodynamically Guided Hierarchical Self-Assembly of Peptide-Protein Bioinks. Advanced Functional Materials, 28(16), Article 1703716. https://doi.org/10.1002/adfm.201703716

Effective integration of molecular self‐assembly and additive manufacturing would provide a technological leap in bioprinting. This article reports on a biofabrication system based on the hydrodynamically guided co‐assembly of peptide amphiphiles (PA... Read More about Hydrodynamically Guided Hierarchical Self-Assembly of Peptide-Protein Bioinks.

RBL cells as models for in vitro studies of mast cells and basophils (2018)
Journal Article
Falcone, F. H., Wan, D., Barwary, N., & Sagi-Eisenberg, R. (2018). RBL cells as models for in vitro studies of mast cells and basophils. Immunological Reviews, 282(1), 47-57. https://doi.org/10.1111/imr.12628

Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically... Read More about RBL cells as models for in vitro studies of mast cells and basophils.

Moving anticoagulation initiation and monitoring services into the community: Evaluation of the Brighton and hove community pharmacy service (2018)
Journal Article
Ingram, S. J., Kirkdale, C. L., Williams, S., Hartley, E., Wintle, S., Sefton, V., & Thornley, T. (2018). Moving anticoagulation initiation and monitoring services into the community: Evaluation of the Brighton and hove community pharmacy service. BMC Health Services Research, 18(1), Article 91. https://doi.org/10.1186/s12913-018-2901-8

Background: As part of the NHS desire to move services closer to where people live, and provide greater accessibility and convenience to patients, Brighton and Hove Clinical Commissioning Group (CCG) underwent a review of their anticoagulation servic... Read More about Moving anticoagulation initiation and monitoring services into the community: Evaluation of the Brighton and hove community pharmacy service.

Multifunctional serine protease inhibitor-coated water-soluble gold nanoparticles as a novel targeted approach for the treatment of inflammatory skin diseases (2018)
Journal Article
Limón, D., Fábrega, M. J., Calpena, A. C., Badia, J., Baldomà, L., & Pérez-García, L. (2018). Multifunctional serine protease inhibitor-coated water-soluble gold nanoparticles as a novel targeted approach for the treatment of inflammatory skin diseases. Bioconjugate Chemistry, 28(4), https://doi.org/10.1021/acs.bioconjchem.7b00717

The overexpression and increased activity of the serine protease Kallikrein 5 (KLK5) is characteristic of inflammatory skin diseases such as Rosacea. The use of inhibitors of this enzyme—such as 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride... Read More about Multifunctional serine protease inhibitor-coated water-soluble gold nanoparticles as a novel targeted approach for the treatment of inflammatory skin diseases.

Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery (2018)
Journal Article
Nieto-Orellana, A., Coghlan, D., Rothery, M., Falcone, F. H., Bosquillon, C., Childerhouse, N., Mantovani, G., & Stolnik, S. (2018). Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery. International Journal of Pharmaceutics, 540(1-2), https://doi.org/10.1016/j.ijpharm.2018.02.008

Pulmonary delivery of protein therapeutics has considerable clinical potential for treating both local and systemic diseases. However, poor protein conformational stability, immunogenicity and protein degradation by proteolytic enzymes in the lung ar... Read More about Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery.

The application of X-ray micro Computed Tomography imaging for tracing particle movement in soil (2018)
Journal Article
Grayling, K., Young, S. D., Roberts, C. J., de Heer, M. I., Shirley, I., Sturrock, C., & Mooney, S. J. (2018). The application of X-ray micro Computed Tomography imaging for tracing particle movement in soil. Geoderma, 321, https://doi.org/10.1016/j.geoderma.2018.01.038

Despite significant advances in recent years in the application of sensing and imaging technologies to the study of the hydraulic behaviour of soils, our understanding of how solutes and particulate matter move through soil is still limited and often... Read More about The application of X-ray micro Computed Tomography imaging for tracing particle movement in soil.

Water solubility enhancement of pyrazolo[3,4-d]pyrimidine derivatives via miniaturized polymer-drug microarrays (2018)
Journal Article
Sanna, M., Sicilia, G., Alazzo, A., Singh, N., Musumeci, F., Schenone, S., Spriggs, K., Burley, J. C., Garnett, M., Taresco, V., & Alexander, C. (in press). Water solubility enhancement of pyrazolo[3,4-d]pyrimidine derivatives via miniaturized polymer-drug microarrays. ACS Medicinal Chemistry Letters, 9(3), https://doi.org/10.1021/acsmedchemlett.7b00456

A miniaturized assay was optimized to evaluate the enhanced apparent water solubility of pyrazolo[3,4-d]pyrimidine derivatives used extensively as anticancer drug scaffolds. The applied amount of drugs used in the reported strategy ranged from 5-10 μ... Read More about Water solubility enhancement of pyrazolo[3,4-d]pyrimidine derivatives via miniaturized polymer-drug microarrays.

Exploring the current and future role of the pharmacists in osteoporosis screening and management in Malaysia (2018)
Journal Article
Toh, L., Lai, P., Othman, S., Shah, A., Dang, C., Low, B., Wong, K., & Anderson, C. (2018). Exploring the current and future role of the pharmacists in osteoporosis screening and management in Malaysia. International Journal of Clinical Pharmacy, 40(2), 450-457. https://doi.org/10.1007/s11096-018-0597-9

Background Several studies have found that pharmacists can assist in screening and prevention of osteoporosis by referring patients for bone mineral density scans and counselling on lifestyle changes. In Malaysia, screening osteoporosis in all elderl... Read More about Exploring the current and future role of the pharmacists in osteoporosis screening and management in Malaysia.

Molecular profiling and combinatorial activity of CCT068127: a potent CDK2 and CDK9 inhibitor (2018)
Journal Article
Whittaker, S. R., Barlow, C., Martin, M. P., Mancusi, C., Wagner, S., Self, A., Barrie, E., te Poele, R., Sharp, S., Brown, N., Wilson, S., Jackson, W., Fischer, P. M., Clarke, P. A., Walton, M. I., McDonald, E., Blagg, J., Noble, M., Garrett, M. D., & Workman, P. (in press). Molecular profiling and combinatorial activity of CCT068127: a potent CDK2 and CDK9 inhibitor. Molecular Oncology, 12(3), https://doi.org/10.1002/1878-0261.12148

Deregulation of the cyclin-dependent kinases (CDKs) has been implicated in the pathogenesis of multiple cancer types. Consequently, CDKs have garnered intense interest as therapeutic targets for the treatment of cancer. We describe herein the molecul... Read More about Molecular profiling and combinatorial activity of CCT068127: a potent CDK2 and CDK9 inhibitor.