All Outputs (224)

A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae (2025)
Journal Article
Scarfe, J., Kosmützky, D., & Nisbet, R. E. R. (2025). A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae. The Plant Journal, 122(6), Article e70272. https://doi.org/10.1111/tpj.70272

Recombinant proteins play a crucial role in both fundamental research and biotechnology. In the laboratory, recombinant proteins are used in a myriad of ways, including to label cells, localize proteins and isolate complexes. In the clinic, antibody-... Read More about A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae.

Quantitative Analysis of Stress Tolerance in Kveik Yeast (2025)
Journal Article
Shirasaya, D., & Powell, C. D. (2025). Quantitative Analysis of Stress Tolerance in Kveik Yeast. Journal of the American Society of Brewing Chemists, 83(3), 317-329. https://doi.org/10.1080/03610470.2025.2491882

Kveik is a term used to describe a group of traditional Saccharomyces cerevisiae strains originally employed by regional Norwegian brewers for the production of malt-based beverages and bread. A key feature of kveik strains is that they perform well... Read More about Quantitative Analysis of Stress Tolerance in Kveik Yeast.

Deciphering the Anti-Listerial Activity and Phytochemical Composition of Licorice Root Extract Using LC-MS/MS in Combination with In Vitro and Computational Evaluations (2025)
Journal Article
Michael, C., Christou, A., Gómez-Caravaca, A. M., Goulas, V., Rees, C. E. D., & Botsaris, G. (2025). Deciphering the Anti-Listerial Activity and Phytochemical Composition of Licorice Root Extract Using LC-MS/MS in Combination with In Vitro and Computational Evaluations. Applied Sciences, 15(10), Article 5276. https://doi.org/10.3390/app15105276

Natural products are an endless source of antimicrobial agents against many foodborne pathogenic bacteria. The present work demonstrates the potential of licorice root extract to efficiently inhibit the growth of a panel of Listeria strains and serot... Read More about Deciphering the Anti-Listerial Activity and Phytochemical Composition of Licorice Root Extract Using LC-MS/MS in Combination with In Vitro and Computational Evaluations.

Prebiotic Galacto-Oligosaccharide and Xylo-Oligosaccharide Feeds in Pig Production: Microbiota Manipulation, Pathogen Suppression, Gut Architecture and Immunomodulatory Effects (2025)
Journal Article
Lee, A., Stanley, J. S., Mellits, K. H., & Connerton, I. F. (2025). Prebiotic Galacto-Oligosaccharide and Xylo-Oligosaccharide Feeds in Pig Production: Microbiota Manipulation, Pathogen Suppression, Gut Architecture and Immunomodulatory Effects. Applied Microbiology, 5(2), Article 42. https://doi.org/10.3390/applmicrobiol5020042

Gastrointestinal health is critical to the productivity and welfare of pigs. The transition from milk to plant-based feeds represents an intestinal challenge at wean that can result in dysbiosis and pathogen susceptibility. Prebiotic galacto-oligosac... Read More about Prebiotic Galacto-Oligosaccharide and Xylo-Oligosaccharide Feeds in Pig Production: Microbiota Manipulation, Pathogen Suppression, Gut Architecture and Immunomodulatory Effects.

Phasing out photosynthesis -and weaponising chlorophyll? (2025)
Journal Article
Howe, C. J., & Nisbet, R. E. R. (2025). Phasing out photosynthesis -and weaponising chlorophyll?. Trends in Parasitology, 41(5), 339-340. https://doi.org/10.1016/j.pt.2025.03.014

Photosynthesis has been lost independently many times in the evolutionary history of eukaryotes. Surprisingly, the ability to synthesise chlorophyll has sometimes been retained after the loss of photosynthesis. Jacko-Reynolds et al. show this has hap... Read More about Phasing out photosynthesis -and weaponising chlorophyll?.

Characterization and therapeutic potential of phage vB_Eco_ZCEC08 against multidrug-resistant uropathogenic Escherichia coli (2025)
Journal Article
Hussein, A. H., Makky, S., Hager, R., Connerton, I. F., & El-Shibiny, A. (2025). Characterization and therapeutic potential of phage vB_Eco_ZCEC08 against multidrug-resistant uropathogenic Escherichia coli. BMC Microbiology, 25(1), Article 221. https://doi.org/10.1186/s12866-025-03903-x

Background

Urinary tract infections (UTIs) caused by antibiotic-resistant bacteria have become a significant public health concern. The increasing ineffectiveness of antibiotics has led to a renewed focus on investigating other strategies, such as... Read More about Characterization and therapeutic potential of phage vB_Eco_ZCEC08 against multidrug-resistant uropathogenic Escherichia coli.

Factors that affect the resilience of young adults to depression: a systematic review (2025)
Journal Article
Theron, L., Stekel, D. J., Höltge, J., Fawole, O. I., Levine, D. T., Mai-Bornu, Z., Maksudi, K., Olaniyan, O., Wright, C. Y., & Ungar, M. (2025). Factors that affect the resilience of young adults to depression: a systematic review. Lancet Psychiatry, 12(5), 377-383. https://doi.org/10.1016/S2215-0366%2825%2900044-6

Depression among young people (aged 18–29 years) transitioning to adulthood is becoming more widespread. Knowing which factors in which systems co-enable resilience to depression is crucial, but there is no comprehensive synthesis of the physiologica... Read More about Factors that affect the resilience of young adults to depression: a systematic review.

Multisystemic resilience and its impact on youth mental health: reflections on co-designing a multi-disciplinary, participatory study (2025)
Journal Article
Theron, L., Bergamini, M., Chambers, C., Choi, K., Fawole, O. I., Fyneface, F. D., Höltge, J., Kapwata, T., Levine, D. T., Mai Bornu, Z., Makape, M., Matross, C., McGrath, B., Olaniyan, O., Stekel, D. J., Hey, J. V., Wright, C. Y., Zion, A. A., & Ungar, M. (2025). Multisystemic resilience and its impact on youth mental health: reflections on co-designing a multi-disciplinary, participatory study. Frontiers in Child and Adolescent Psychiatry, 4, Article 1489950. https://doi.org/10.3389/frcha.2025.1489950

Youth depression is a global emergency. Redressing this emergency requires a sophisticated understanding of the multisystemic risks and biopsychosocial, economic, and environmental resources associated with young people's experiences of no/limited ve... Read More about Multisystemic resilience and its impact on youth mental health: reflections on co-designing a multi-disciplinary, participatory study.

Genomic and phenotypic characterisation of Pseudomonas aeruginosa isolates from canine otitis externa reveals high-risk sequence types identical to those found in human nosocomial infections (2025)
Journal Article
Secker, B., Shaw, S., Hobley, L., & Atterbury, R. J. (2025). Genomic and phenotypic characterisation of Pseudomonas aeruginosa isolates from canine otitis externa reveals high-risk sequence types identical to those found in human nosocomial infections. Frontiers in Microbiology, 16, Article 1526843. https://doi.org/10.3389/fmicb.2025.1526843

Introduction:

Canine otitis externa (OE) is a frequently-diagnosed condition in veterinary practices worldwide. Pseudomonas aeruginosa is commonly associated with chronic and recalcitrant canine OE, but studies with detailed genomic and phenotypi... Read More about Genomic and phenotypic characterisation of Pseudomonas aeruginosa isolates from canine otitis externa reveals high-risk sequence types identical to those found in human nosocomial infections.

Potential of vB_Pa_ZCPS1 phage embedded in situ gelling formulations as an ocular delivery system to attenuate Pseudomonas aeruginosa keratitis in a rabbit model (2025)
Journal Article
Abdelrahman, F., Makky, S., Teba, H. E., Abd ElAziz, M. M., Agwa, M. M., Awad, R., Hassan, Y. Y., Abdelsattar, A. S., Connerton, I. F., & El-Shibiny, A. (2025). Potential of vB_Pa_ZCPS1 phage embedded in situ gelling formulations as an ocular delivery system to attenuate Pseudomonas aeruginosa keratitis in a rabbit model. Journal of Controlled Release, 380, 52-70. https://doi.org/10.1016/j.jconrel.2025.01.091

Pseudomonas aeruginosa keratitis (or pink eye) is a challenging ocular infection that causes serious complications due to the deficiency of effective antibiotic treatment. Thus, in this study we isolated and characterized a specific bacteriophage, ph... Read More about Potential of vB_Pa_ZCPS1 phage embedded in situ gelling formulations as an ocular delivery system to attenuate Pseudomonas aeruginosa keratitis in a rabbit model.

Valorisation of hop leaves for their bioactive compounds: Identification and quantification of phenolics across different varieties, crop years and stages of development (2024)
Journal Article
Calvert, D., Dew, T., Gadon, A., Gros, J., & Cook, D. (2025). Valorisation of hop leaves for their bioactive compounds: Identification and quantification of phenolics across different varieties, crop years and stages of development. Food Chemistry, 465(Part 2), Article 142005. https://doi.org/10.1016/j.foodchem.2024.142005

Hop leaves, a by-product from hop cone harvesting, contain phenolic compounds of potential value for food or beverage applications. However, the abundant phenolics in hop leaves remain largely unquantified. This study quantified phenolics in hop leav... Read More about Valorisation of hop leaves for their bioactive compounds: Identification and quantification of phenolics across different varieties, crop years and stages of development.

Multiplexing projects: supervising undergraduate research projects with larger cohorts (2024)
Journal Article
Nisbet, R. E. R., Scott, D. J., & Campeotto, I. (2024). Multiplexing projects: supervising undergraduate research projects with larger cohorts. The Biochemist, 46(5), 18-23. https://doi.org/10.1042/BIO_2024_897

Almost all undergraduate students in the UK complete a final year research project or dissertation. In the molecular biosciences field, these projects are often based in individual research laboratory. However, with increasing student numbers, fallin... Read More about Multiplexing projects: supervising undergraduate research projects with larger cohorts.

A histopathological study in road-killed European badgers (Meles meles) from the English midlands with isolation of novel non-tuberculous atypical mycobacteria (2024)
Journal Article
Corbetta, D., Grau-Roma, L., Rees, C., Swift, B. M. C., O’Cathail, C., Barron, E. S., Verin, R., Morey-Matamalas, A., Sorley, M., & Bennett, M. (2024). A histopathological study in road-killed European badgers (Meles meles) from the English midlands with isolation of novel non-tuberculous atypical mycobacteria. European Journal of Wildlife Research, 70(6), Article 112. https://doi.org/10.1007/s10344-024-01866-4

European badgers (Meles meles) play an important role in the epidemiology of bovine tuberculosis (caused by Mycobacterium bovis) in England, but little is known about the prevalence of atypical mycobacteriosis. Badgers are also known to be infected b... Read More about A histopathological study in road-killed European badgers (Meles meles) from the English midlands with isolation of novel non-tuberculous atypical mycobacteria.

Impacts of starch breakdown during germination and stewing on aroma formation in crystal malts (2024)
Journal Article
Foulkes, A. R., Bolat, I., Dodds, C., & Cook, D. J. (2024). Impacts of starch breakdown during germination and stewing on aroma formation in crystal malts. Journal of the Institute of Brewing, 130(4), 221-237. https://doi.org/10.58430/jib.v130i4.60

Why was the work done: Crystal malts are used in brewing to add colour, flavour and mouthfeel to beers. In their manufacture, aroma compounds are formed through thermal flavour generation during roasting of stewed green malt. As part of a wider proje... Read More about Impacts of starch breakdown during germination and stewing on aroma formation in crystal malts.

Extreme enthalpy‒entropy compensation in the dimerization of small solutes in aqueous solution (2024)
Journal Article
Scott, D. J., & Winzor, D. J. (2024). Extreme enthalpy‒entropy compensation in the dimerization of small solutes in aqueous solution. European Biophysics Journal, 53, 373-384. https://doi.org/10.1007/s00249-024-01722-y

This communication summarizes findings from the earliest encounters with extreme enthalpy‒entropy compensation, a phenomenon first detected in the 1950s by a reappraisal of isopiestic and calorimetric measurements on aqueous urea solutions in terms o... Read More about Extreme enthalpy‒entropy compensation in the dimerization of small solutes in aqueous solution.

Bacteriophage Control of Foodborne Pathogens in Food Production (2024)
Book Chapter
Liang, L., & Connerton, I. F. (2024). Bacteriophage Control of Foodborne Pathogens in Food Production. In Z. Lu, & Y. Lu (Eds.), Bio-Based Antimicrobial Agents to Improve Agricultural and Food Safety (256-282). Bentham Science Publishers. https://doi.org/10.2174/9789815256239124010010

The application of bacteriophages (phages) that target and kill bacteria to safeguard foods and food production facilities has attracted attention over the last decade. As phages are often already present in foods and food production settings, their... Read More about Bacteriophage Control of Foodborne Pathogens in Food Production.

Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5 (2024)
Journal Article
Harrison, T. E., Alam, N., Farrell, B., Quinkert, D., Lias, A. M., King, L. D., Barfod, L. K., Draper, S. J., Campeotto, I., & Higgins, M. K. (2024). Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5. EMBO Molecular Medicine, 16(10), 2539-2559. https://doi.org/10.1038/s44321-024-00123-0

There is an urgent need for improved malaria vaccine immunogens. Invasion of erythrocytes by Plasmodium falciparum is essential for its life cycle, preceding symptoms of disease and parasite transmission. Antibodies which target PfRH5 are highly effe... Read More about Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5.

Is there reversible dimerization of albumin in blood plasma? And does it matter? (2024)
Journal Article
Harris, G., Bradshaw, M. L., Halsall, D. J., Scott, D. J., Unwin, R. J., & Norden, A. G. W. (2024). Is there reversible dimerization of albumin in blood plasma? And does it matter?. Experimental Physiology, 109(10), 1663-1671. https://doi.org/10.1113/EP092012

Most albumin in blood plasma is thought to be monomeric with some 5% covalently dimerized. However, many reports in the recent biophysics literature find that albumin is reversibly dimerized or even oligomerized. We review data on this from X‐ray cry... Read More about Is there reversible dimerization of albumin in blood plasma? And does it matter?.

Prebiotic galactooligosaccharide feed modifies the chicken gut microbiota to efficiently clear Salmonella (2024)
Journal Article
Richards, P. J., Almutrafy, A., Liang, L., Flaujac Lafontaine, G. M., King, E., Fish, N. M., Connerton, A. J., Connerton, P. L., & Connerton, I. F. (2024). Prebiotic galactooligosaccharide feed modifies the chicken gut microbiota to efficiently clear Salmonella. mSystems, 9(8), Article e00754-24. https://doi.org/10.1128/msystems.00754-24

Chicken meat is contaminated with Salmonella from the gut of infected chickens during slaughter. Eradication of Salmonella from broiler chickens through hygiene measures and/or vaccination is not cost-effective; complementary approaches are required.... Read More about Prebiotic galactooligosaccharide feed modifies the chicken gut microbiota to efficiently clear Salmonella.

Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region. (2024)
Journal Article
Mao, Y., Dewi, S. R., Harding, S. E., & Binner, E. (2024). Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region. Food Chemistry, 460(Pt 1), Article 140549. https://doi.org/10.1016/j.foodchem.2024.140549

This work investigated a sustainable and efficient approach of pectin extraction for banana peel waste valorisation and studied the influence of banana ripening stages (RS at 2,5 and 7). Although pectin content in banana peel raw material decreased d... Read More about Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region..