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A new high-performance heterologous fungal expression system based on regulatory elements from the Aspergillus terreus terrein gene cluster (2015)
Journal Article
Gressler, M., Hortschansky, P., Geib, E., & Brock, M. (2015). A new high-performance heterologous fungal expression system based on regulatory elements from the Aspergillus terreus terrein gene cluster. Frontiers in Microbiology, 6, https://doi.org/10.3389/fmicb.2015.00184

Recently, the Aspergillus terreus terrein gene cluster was identified and selected for development of a new heterologous expression system. The cluster encodes the specific transcription factor TerR that is indispensable for terrein cluster induction... Read More about A new high-performance heterologous fungal expression system based on regulatory elements from the Aspergillus terreus terrein gene cluster.

Investigating the field-dependence of the Davis model: Calibrated fMRI at 1.5, 3 and 7 T (2015)
Journal Article
Hare, H. V., Blockley, N. P., Gardener, A. G., Clare, S., & Bulte, D. P. (2015). Investigating the field-dependence of the Davis model: Calibrated fMRI at 1.5, 3 and 7 T. NeuroImage, 112, 189-196. https://doi.org/10.1016/j.neuroimage.2015.02.068

Gas calibrated fMRI in its most common form uses hypercapnia in conjunction with the Davis model to quantify relative changes in the cerebral rate of oxygen consumption (CMRO2) in response to a functional stimulus. It is most commonly carried out at... Read More about Investigating the field-dependence of the Davis model: Calibrated fMRI at 1.5, 3 and 7 T.

Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells (2015)
Journal Article
Jean-Gilles, L., Braitch, M., Latif, M. L., Aram, J., Fahey, A. J., Edwards, L. J., …Constantinescu, C. S. (2015). Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells. Acta Physiologica, 214(1), 63-74. https://doi.org/10.1111/apha.12474

© 2015 The Authors. Aims: To investigate the regulation of cannabinoid receptors CB1 and CB2 on immune cells by pro-inflammatory cytokines and its potential relevance to the inflammatory neurological disease, multiple sclerosis (MS). CB1 and CB2 sign... Read More about Effects of pro-inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells.

Differential contributions of A- and C-nociceptors to primary and secondary inflammatory hypersensitivity in the rat (2015)
Journal Article
Hsieh, M.-T., Donaldson, L. F., & Lumb, B. M. (2015). Differential contributions of A- and C-nociceptors to primary and secondary inflammatory hypersensitivity in the rat. PAIN, 156(6), 1. https://doi.org/10.1097/j.pain.0000000000000151

Primary hyperalgesia is characterized by increased responsiveness to both heat and mechanical stimulation in the area of injury. By contrast, secondary hyperalgesia is generally associated with increased responses to mechanical but not heat stimuli.... Read More about Differential contributions of A- and C-nociceptors to primary and secondary inflammatory hypersensitivity in the rat.

Co-infections determine patterns of mortality in a population exposed to parasite infection (2015)
Journal Article
Woolhouse, M. E., Thumbi, S. M., Jennings, A., Chase-Topping, M., Callaby, R., Kiara, H., …Toye, P. G. (2015). Co-infections determine patterns of mortality in a population exposed to parasite infection. Science Advances, 1(2), Article e1400026. https://doi.org/10.1126/sciadv.1400026

© 2015 The Authors, some rights reserved. Many individual hosts are infected with multiple parasite species, and this may increase or decrease the pathogenicity of the infections. This phenomenon is termed heterologous reactivity and is potentially a... Read More about Co-infections determine patterns of mortality in a population exposed to parasite infection.

Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-γ-treated macrophages to pseudomonas aeruginosa infection (2015)
Journal Article
Singh, S., Barr, H. L., Liu, Y.-C., Robins, A., Heeb, S., Williams, P., …Martinez-Pomares, L. (2015). Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-γ-treated macrophages to pseudomonas aeruginosa infection. PLoS ONE, 10(2), Article e0117447. https://doi.org/10.1371/journal.pone.0117447

Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe infections at compromised epithelial surfaces, such those found in burns, wounds, and in lungs damaged by mechanical ventilation or recurrent infections, particularly in cystic... Read More about Granulocyte-macrophage colony stimulatory factor enhances the pro-inflammatory response of interferon-γ-treated macrophages to pseudomonas aeruginosa infection.

Calcitonin gene-related peptide-expressing sensory neurons and spinal microglial reactivity contribute to pain states in collagen-induced arthritis (2015)
Journal Article
Nieto, F. R., Clark, A. K., Grist, J., Chapman, V., & Malcangio, M. (2015). Calcitonin gene-related peptide-expressing sensory neurons and spinal microglial reactivity contribute to pain states in collagen-induced arthritis. Arthritis and Rheumatology, 67(6), https://doi.org/10.1002/art.39082

Objective To evaluate the contribution of sensory neurons in ankle joints and adjacent tissue to the development of pain in collagen‐induced arthritis (CIA), and to determine the relationship between pain and the appearance of clinical signs.... Read More about Calcitonin gene-related peptide-expressing sensory neurons and spinal microglial reactivity contribute to pain states in collagen-induced arthritis.

The mammalian target of rapamycin complex 1 (mTORC1) in breast cancer: the impact of oestrogen receptor and HER2 pathways (2015)
Journal Article
Jerjees, D. A., Negm, O. H., Alabdullah, M. L., Mirza, S., Alkaabi, M., Hameed, M. R., …Rakha, E. A. (2015). The mammalian target of rapamycin complex 1 (mTORC1) in breast cancer: the impact of oestrogen receptor and HER2 pathways. Breast Cancer Research and Treatment, 150(1), 91-103. https://doi.org/10.1007/s10549-015-3308-4

The mammalian target of rapamycin complex 1 (mTORC1) is a downstream of the PI3K/Akt pathway which affects cancer development. mTORC1 has many downstream signalling effectors that can enhance different cellular responses. This study aims to investiga... Read More about The mammalian target of rapamycin complex 1 (mTORC1) in breast cancer: the impact of oestrogen receptor and HER2 pathways.

Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry (2015)
Journal Article
Herod, M. R., Pineda, R. G., Mautner, V., & Onion, D. (2015). Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry. Small, 11(7), https://doi.org/10.1002/smll.201401885

A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of technique... Read More about Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry.

Effects of receptor tyrosine kinase inhibitors on VEGF165a- and VEGF165b-stimulated gene transcription in HEK-293 cells expressing human VEGFR2 (2015)
Journal Article
Carter, J. J., Wheal, A. J., Hill, S. J., & Woolard, J. (2015). Effects of receptor tyrosine kinase inhibitors on VEGF165a- and VEGF165b-stimulated gene transcription in HEK-293 cells expressing human VEGFR2. British Journal of Pharmacology, 172(12), https://doi.org/10.1111/bph.13116

BACKGROUND AND PURPOSE: Receptor tyrosine kinase inhibitors (RTKIs) targeted at VEGF receptor 2 (VEGFR2) have proved to be attractive approaches to cancer therapy based on their ability to reduce angiogenesis. Here we have undertaken a quantitative... Read More about Effects of receptor tyrosine kinase inhibitors on VEGF165a- and VEGF165b-stimulated gene transcription in HEK-293 cells expressing human VEGFR2.

Evidence of introgressive hybridization between the morphologically divergent land snails Ainohelix and Ezohelix (2015)
Journal Article
Morii, Y., Yokoyama, J., Kawata, M., Davison, A., & Satoshi, C. (2015). Evidence of introgressive hybridization between the morphologically divergent land snails Ainohelix and Ezohelix. Biological Journal of the Linnean Society, 115(1), https://doi.org/10.1111/bij.12466

Hybridization between different taxa is likely to take place when adaptive morphological differences evolve more rapidly than reproductive isolation. When studying the phylogenetic relationship between two land snails of different nominal genera, Ain... Read More about Evidence of introgressive hybridization between the morphologically divergent land snails Ainohelix and Ezohelix.

Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters (2015)
Journal Article
Khairalla, A. S., Omer, S. A., Mahdavi, J., Aslam, A., Dufailu, O. A., Self, T., …Ala'Aldeen, D. A. (2015). Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters. Cellular Microbiology, 17(7), 1008-1020. https://doi.org/10.1111/cmi.12417

Neisseria meningitidis, a major cause of bacterial meningitis and septicaemia, secretes multiple virulence factors, including the adhesion and penetration protein (App) and meningococcal serine protease A (MspA). Both are conserved, immunogenic, type... Read More about Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters.

Microfluidics for advanced drug delivery systems (2015)
Journal Article
Riahi, R., Tamayol, A., Shaegh, S. A. M., Ghaemmaghami, A. M., Dokmeci, M. R., & Khademshosseini, A. (2015). Microfluidics for advanced drug delivery systems. Current Opinion in Chemical Engineering, 7, 101-112. https://doi.org/10.1016/j.coche.2014.12.001

©2015 Elsevier Ltd. All rights reserved. Considerable efforts have been devoted toward developing effective drug delivery methods. Microfluidic systems, with their capability for precise handling and transport of small liquid quantities, have emerged... Read More about Microfluidics for advanced drug delivery systems.

Salmeterol's extreme b2 selectivity is due to residues in both extracellular loops and transmembrane domains (2015)
Journal Article
Baker, J. G., Proudman, R. G., & Hill, S. J. (2015). Salmeterol's extreme b2 selectivity is due to residues in both extracellular loops and transmembrane domains. Molecular Pharmacology, 87(1), https://doi.org/10.1124/mol.114.095364

Salmeterol is a long-acting b2-agonist, widely used as an inhaled treatment of asthma and chronic obstructive pulmonary disease. It has very high b2-affinity (log KD 28.95) and is very selective for the b2-adrenoceptor (1000-fold selectivity over the... Read More about Salmeterol's extreme b2 selectivity is due to residues in both extracellular loops and transmembrane domains.

A G protein-coupled receptor dimer imaging assay reveals selectively modified pharmacology of neuropeptide Y Y1/Y5 receptor heterodimers (2015)
Journal Article
Kilpatrick, L. E., Humphrys, L. J., & Holliday, N. D. (in press). A G protein-coupled receptor dimer imaging assay reveals selectively modified pharmacology of neuropeptide Y Y1/Y5 receptor heterodimers. Molecular Pharmacology, 87(4), https://doi.org/10.1124/mol.114.095356

The ability of G protein-coupled receptors (GPCRs) to form dimers, and particularly heterodimers, offers potential for targeted therapeutics with improved selectivity. However, studying dimer pharmacology is challenging, because of signaling cross-ta... Read More about A G protein-coupled receptor dimer imaging assay reveals selectively modified pharmacology of neuropeptide Y Y1/Y5 receptor heterodimers.

Proof of concept study for designed multiple ligands targeting the dopamine D2, serotonin 5-HT2A, and muscarinic M1 acetylcholine receptors (2015)
Journal Article
Szabo, M., Lim, H. D., Herenbrink, C. K., Christopoulos, A., Lane, J. R., & Capuano, B. (2015). Proof of concept study for designed multiple ligands targeting the dopamine D2, serotonin 5-HT2A, and muscarinic M1 acetylcholine receptors. Journal of Medicinal Chemistry, 58(3), 1550-1555. https://doi.org/10.1021/jm5013243

Herein we describe the hybridization of a benzoxazinone M1 scaffold with D2 privileged structures derived from putative and clinically relevant antipsychotics to develop designed multiple ligands. The M1 mAChR is an attractive target for the cognitiv... Read More about Proof of concept study for designed multiple ligands targeting the dopamine D2, serotonin 5-HT2A, and muscarinic M1 acetylcholine receptors.

Human lectins and their roles in viral infections (2015)
Journal Article
Mason, C. P., & Tarr, A. W. (2015). Human lectins and their roles in viral infections. Molecules, 20(2), 2229-2271. https://doi.org/10.3390/molecules20022229

© 2015 by the authors; licensee MDPI. Innate recognition of virus proteins is an important component of the immune response to viral pathogens. A component of this immune recognition is the family of lectins; pattern recognition receptors (PRRs) that... Read More about Human lectins and their roles in viral infections.

An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids (2015)
Journal Article
Amey, J. S., O'Reilly, A. O., Burton, M. J., Puinean, A. M., Mellor, I. R., Duce, I. R., …Davies, T. E. (2015). An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids. FEBS Letters, 589(5), 598-607. https://doi.org/10.1016/j.febslet.2015.01.020

We describe the identification in aphids of a unique heterodimeric voltage-gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. We demonstrate that this channel h... Read More about An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids.

Statin myalgia is not associated with reduced muscle strength, mass or protein turnover in older male volunteers, but is allied with a slowing of time to peak power output, insulin resistance and differential muscle mRNA expression: Statin myalgia, muscle function and metabolism (2015)
Journal Article
Selby, A., Murton, A., Mallinson, J. E., Marimuthu, K., Murton, A. J., Selby, A. L., …Greenhaff, P. L. (2015). Statin myalgia is not associated with reduced muscle strength, mass or protein turnover in older male volunteers, but is allied with a slowing of time to peak power output, insulin resistance and differential muscle mRNA expression: Statin myalgia, muscle function and metabolism. Journal of Physiology, 593(5), 1239-1257. https://doi.org/10.1113/jphysiol.2014.285577

Statins are associated with muscle myalgia and myopathy, which probably reduce habitual physical activity. This is particularly relevant to older people who are less active, sarcopaenic and at increased risk of statin myalgia. We hypothesised that st... Read More about Statin myalgia is not associated with reduced muscle strength, mass or protein turnover in older male volunteers, but is allied with a slowing of time to peak power output, insulin resistance and differential muscle mRNA expression: Statin myalgia, muscle function and metabolism.