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RpoS differentially affects the general stress response and biofilm formation in the endophytic Serratia plymuthica G3 (2015)
Journal Article

The ?S subunit RpoS of RNA polymerase functions as a master regulator of the general stress response in Escherichia coli and related bacteria. RpoS has been reported to modulate biocontrol properties in the rhizobacterium Serratia plymuthica IC1270.... Read More about RpoS differentially affects the general stress response and biofilm formation in the endophytic Serratia plymuthica G3.

Synthesis, Biological Evaluation, and Utility of Fluorescent Ligands Targeting the μ-Opioid Receptor (2015)
Journal Article
Schembri, L. S., Stoddart, L. A., Briddon, S. J., Kellam, B., Canals, M., Graham, B., & Scammells, P. J. (2015). Synthesis, Biological Evaluation, and Utility of Fluorescent Ligands Targeting the μ-Opioid Receptor. Journal of Medicinal Chemistry, 58(24), 9754-9767. https://doi.org/10.1021/acs.jmedchem.5b01664

Fluorescently labeled ligands are useful pharmacological research tools for studying receptor localization, trafficking, and signaling processes via fluorescence imaging. They are also employed in fluorescent binding assays. This study is centered on... Read More about Synthesis, Biological Evaluation, and Utility of Fluorescent Ligands Targeting the μ-Opioid Receptor.

Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus (2015)
Journal Article
Lambert, C., Cadby, I. T., Till, R., Bui, N. K., Lerner, T. R., Hughes, W. S., …Lovering, A. L. (2015). Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus. Nature Communications, 6, Article 8884. https://doi.org/10.1038/ncomms9884

Predatory Bdellovibrio bacteriovorus are natural antimicrobial organisms, killing other bacteria by whole-cell invasion. Self-protection against prey-metabolizing enzymes is important for the evolution of predation. Initial prey entry involves the pr... Read More about Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus.

The antimalarial drug primaquine targets Fe–S cluster proteins and yeast respiratory growth (2015)
Journal Article
Lalève, A., Vallieres, C., Golinelli-Cohen, M.-P., Bouton, C., Song, Z., Pawlik, G., …Meunier, B. (2016). The antimalarial drug primaquine targets Fe–S cluster proteins and yeast respiratory growth. Redox Biology, 7, https://doi.org/10.1016/j.redox.2015.10.008

Malaria is a major health burden in tropical and subtropical countries. The antimalarial drug primaquine is extremely useful for killing the transmissible gametocyte forms of Plasmodium falciparum and the hepatic quiescent forms of P. vivax. Yet its... Read More about The antimalarial drug primaquine targets Fe–S cluster proteins and yeast respiratory growth.

β2-adrenoceptor signaling regulates invadopodia formation to enhance tumor cell invasion (2015)
Journal Article
Creed, S. J., Le, C. P., Hassan, M., Pon, C. K., Albold, S., Chan, K. T., …Sloan, E. K. (2015). β2-adrenoceptor signaling regulates invadopodia formation to enhance tumor cell invasion. Breast Cancer Research, 17(1), Article 145. https://doi.org/10.1186/s13058-015-0655-3

INTRODUCTION:For efficient metastatic dissemination, tumor cells form invadopodia to degrade and move through three-dimensional extracellular matrix. However, little is known about the conditions that favor invadopodia formation. Here, we investigate... Read More about β2-adrenoceptor signaling regulates invadopodia formation to enhance tumor cell invasion.

The limitations of in vitro experimentation in understanding biofilms and chronic infection (2015)
Journal Article
Roberts, A., Kragh, K., Bjarnsholt, T., & Diggle, S. P. (2015). The limitations of in vitro experimentation in understanding biofilms and chronic infection. Journal of Molecular Biology, 427(23), https://doi.org/10.1016/j.jmb.2015.09.002

We have become increasingly aware that during infection, pathogenic bacteria often grow in multi- cellular biofilms which are often highly resistant to antibacterial strategies. In order to understand how biofilms form and contribute to infection, in... Read More about The limitations of in vitro experimentation in understanding biofilms and chronic infection.

Novel, synergistic antifungal combinations that target translation fidelity (2015)
Journal Article
Moreno-Martinez, E., Vallieres, C., Holland, S. L., & Avery, S. V. (in press). Novel, synergistic antifungal combinations that target translation fidelity. Scientific Reports, 5(16700), https://doi.org/10.1038/srep16700

There is an unmet need for new antifungal or fungicide treatments, as resistance to existing treatments grows. Combination treatments help to combat resistance. Here we develop a novel, effective target for combination antifungal therapy. Different a... Read More about Novel, synergistic antifungal combinations that target translation fidelity.

Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity (2015)
Journal Article
Ivančić-Baće, I., Cass, S. D., Wearne, S. J., & Bolt, E. L. (2015). Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research, 43(22), 10821-10830. https://doi.org/10.1093/nar/gkv1213

CRISPR-Cas is a prokaryotic immune system built from capture and integration of invader DNA into CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci, termed ‘Adaptation’, which is dependent on Cas1 and Cas2 proteins. In Escherichi... Read More about Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity.

Antibody-mediated inhibition of the FGFR1c isoform induces a catabolic lean state in Siberian hamsters (2015)
Journal Article
Samms, R. J., Lewis, J. E., Lory, A., Fowler, M. J., Cooper, S., Warner, A., …Ebling, F. J. (2015). Antibody-mediated inhibition of the FGFR1c isoform induces a catabolic lean state in Siberian hamsters. Current Biology, 25(22), https://doi.org/10.1016/j.cub.2015.10.010

Hypothalamic tanycytes are considered to function as sensors of peripheral metabolism [1]. To facilitate this role, they express a wide range of receptors, including fibroblast growth factor receptor 1 (FGFR1). Using a monoclonal antibody (IMC-H7) th... Read More about Antibody-mediated inhibition of the FGFR1c isoform induces a catabolic lean state in Siberian hamsters.

Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes (2015)
Journal Article
Roques, M., Wall, R. J., Douglass, A. P., Ramaprasad, A., Ferguson, D. J. P., Kaindama, M. L., …Tewari, R. (2015). Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes. PLoS Pathogens, 11(11), Article e1005273. https://doi.org/10.1371/journal.ppat.1005273

Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisi... Read More about Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes.

Identification of the ISWI chromatin remodeling complex of the early branching Eukaryote Trypanosoma brucei (2015)
Journal Article
Stanne, T., Shankar Narayanan, M., Ridewood, S., Ling, A., Witmer, K., Kushwaha, M., …Rudenko, G. (2015). Identification of the ISWI chromatin remodeling complex of the early branching Eukaryote Trypanosoma brucei. Journal of Biological Chemistry, 290, https://doi.org/10.1074/jbc.M115.679019

ISWI chromatin remodelers are highly conserved in eukaryotes and are important for the assembly and spacing of nucleosomes, thereby controlling transcription initiation and elongation. ISWI is typically associated with different subunits, forming sp... Read More about Identification of the ISWI chromatin remodeling complex of the early branching Eukaryote Trypanosoma brucei.

Simulating the influence of plasma protein on measured receptor affinity in biochemical assays reveals the utility of Schild analysis for estimating compound affinity for plasma proteins (2015)
Journal Article
Blakeley, D., Sykes, D. A., Ensor, P., Bertran, E., Aston, P. J., & Charlton, S. J. (2015). Simulating the influence of plasma protein on measured receptor affinity in biochemical assays reveals the utility of Schild analysis for estimating compound affinity for plasma proteins. British Journal of Pharmacology, 172(21), 5037-5049. https://doi.org/10.1111/bph.13263

© 2015 The British Pharmacological Society. Background and Purpose Plasma protein binding (PPB) influences the free fraction of drug available to bind to its target and is therefore an important consideration in drug discovery. While traditional meth... Read More about Simulating the influence of plasma protein on measured receptor affinity in biochemical assays reveals the utility of Schild analysis for estimating compound affinity for plasma proteins.

Synthesis and cell-free cloning of DNA libraries using programmable microfluidics (2015)
Journal Article
Ben Yehezkel, T., Rival, A., Raz, O., Cohen, R., Marx, Z., Cámara, M., …Shapiro, E. (2016). Synthesis and cell-free cloning of DNA libraries using programmable microfluidics. Nucleic Acids Research, 44(4), e35. https://doi.org/10.1093/nar/gkv1087

Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libra... Read More about Synthesis and cell-free cloning of DNA libraries using programmable microfluidics.

Genome-wide analysis reveals the extent of EAV-HP integration in domestic chicken (2015)
Journal Article
Wragg, D., Mason, A. S., Yu, L., Kuo, R., Lawal, R. A., Desta, T. T., …Hanotte, O. (2015). Genome-wide analysis reveals the extent of EAV-HP integration in domestic chicken. BMC Genomics, 16, Article 784. https://doi.org/10.1186/s12864-015-1954-x

Background: EAV-HP is an ancient retrovirus pre-dating Gallus speciation, which continues to circulate in modern chicken populations, and led to the emergence of avian leukosis virus subgroup J causing significant economic losses to the poultry indus... Read More about Genome-wide analysis reveals the extent of EAV-HP integration in domestic chicken.

Reconstruction and Simulation of Neocortical Microcircuitry (2015)
Journal Article
Graham, J., Gevaert, M., Berger, T., Kahou, G., Sanchez, C., Reimann, M., …Schürmann, F. (2015). Reconstruction and Simulation of Neocortical Microcircuitry. Cell, 163(2), 456-492. https://doi.org/10.1016/j.cell.2015.09.029

We present a first-draft digital reconstruction of the microcircuitry of somatosensory cortex of juvenile rat. The reconstruction uses cellular and synaptic organizing principles to algorithmically reconstruct detailed anatomy and physiology from spa... Read More about Reconstruction and Simulation of Neocortical Microcircuitry.

Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques (2015)
Journal Article
Wong, K., Briddon, S. J., Holliday, N. D., & Kerr, I. D. (2016). Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1863(1), 19-29. https://doi.org/10.1016/j.bbamcr.2015.10.002

ABCG2 is one of three human ATP binding cassette (ABC) transporters involved in the export from cells of a chemically and structurally diverse range of compounds. This multidrug efflux capability, together with a broad tissue distribution in the body... Read More about Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques.