All Outputs (2211)

Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the humanβ1-adrenoceptor expressed in HEK-293 cells (2016)
Journal Article
Soave, M., Stoddart, L. A., Brown, A., Woolard, J., & Hill, S. J. (2016). Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the humanβ1-adrenoceptor expressed in HEK-293 cells. Pharmacology Research and Perspectives, 4(5), Article e00250. https://doi.org/10.1002/prp2.250

Previous research has indicated that allosteric interactions across the dimer interface of β1-adrenoceptors may be responsible for a secondary low affinity binding conformation. Here we have investigated the potential for probe dependence, in the det... Read More about Use of a new proximity assay (NanoBRET) to investigate the ligand-binding characteristics of three fluorescent ligands to the humanβ1-adrenoceptor expressed in HEK-293 cells.

Robust anti-nociceptive effects of MAG lipase inhibition in a model of osteoarthritis pain (2016)
Journal Article
Burston, J. J., Mapp, P. I., Sarmad, S., Barrett, D. A., Niphakis, M. J., Cravatt, B. F., …Chapman, V. (2016). Robust anti-nociceptive effects of MAG lipase inhibition in a model of osteoarthritis pain. British Journal of Pharmacology, 173(21), 3134-3144. https://doi.org/10.1111/bph.13574

BACKGROUND AND PURPOSE: Chronic pain is often a symptom of knee osteoarthritis (OA) for which current analgesics are either inadequate, or are associated with serious side effects. The endocannabinoid system may offer alternative targets for pain-rel... Read More about Robust anti-nociceptive effects of MAG lipase inhibition in a model of osteoarthritis pain.

Interaction of ARC and Daxx: a novel endogenous target to preserve motor function and cell loss after focal brain ischemia in mice (2016)
Journal Article
Donath, S., An, J., Lee, S. L. L., Gertz, K., Datwyler, A. L., Harms, U., …Harms, C. (2016). Interaction of ARC and Daxx: a novel endogenous target to preserve motor function and cell loss after focal brain ischemia in mice. Journal of Neuroscience, 36(31), 8132-8148. https://doi.org/10.1523/JNEUROSCI.4428-15.2016

The aim of this study was to explore the signaling and neuroprotective effect of transactivator of transcription (TAT) protein transduction of the apoptosis repressor with CARD (ARC) in in vitro and in vivo models of cerebral ischemia in mice. In mic... Read More about Interaction of ARC and Daxx: a novel endogenous target to preserve motor function and cell loss after focal brain ischemia in mice.

Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing (2016)
Journal Article
Sifrim, A., Hitz, M.-P., Wilsdon, A., Breckpot, J., Turki, S. H. A., Thienpont, B., …Hurles, M. E. (2016). Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing. Nature Genetics, 48(9), 1060-1065. https://doi.org/10.1038/ng.3627

Congenital heart defects (CHDs) have a neonatal incidence of 0.8–1%. Despite abundant examples of monogenic CHD in humans and mice, CHD has a low absolute sibling recurrence risk (~2.7%), suggesting a considerable role for de novo mutations (DNMs) an... Read More about Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing.

Distribution and trafficking of the μ-opioid receptor in enteric neurons of the guinea pig (2016)
Journal Article
Lay, J., Carbone, S. E., DiCello, J. J., Bunnett, N. W., Canals, M., & Poole, D. P. (2016). Distribution and trafficking of the μ-opioid receptor in enteric neurons of the guinea pig. AJP - Gastrointestinal and Liver Physiology, 311(2), G252-G266. https://doi.org/10.1152/ajpgi.00184.2016

© 2016 the American Physiological Society. The μ-opioid receptor (MOR) is a major regulator of gastrointestinal motility and secretion and mediates opiate-induced bowel dysfunction. Although MOR is of physiological and therapeutic importance to gut f... Read More about Distribution and trafficking of the μ-opioid receptor in enteric neurons of the guinea pig.

A genetic assay for gene essentiality in Clostridium (2016)
Journal Article
Walker, D. J., Heap, J. T., Winzer, K., & Minton, N. P. (2016). A genetic assay for gene essentiality in Clostridium. Anaerobe, 42, 40-43. https://doi.org/10.1016/j.anaerobe.2016.07.007

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium d... Read More about A genetic assay for gene essentiality in Clostridium.

Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond (2016)
Journal Article
Phillips, M. A., Van Voorhis, W. C., Adams, J. H., Adelfio, R., Ahyong, V., Akabas, M. H., …Willis, P. A. (2016). Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond. PLoS Pathogens, 12(7), Article e1005763. https://doi.org/10.1371/journal.ppat.1005763

A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of medicinal chemistry is still larg... Read More about Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond.

Ultrasound induced fluorescence of nanoscale liposome contrast agents (2016)
Journal Article
Zhang, Q., Morgan, S. P., O’Shea, P., & Mather, M. L. (2016). Ultrasound induced fluorescence of nanoscale liposome contrast agents. PLoS ONE, 11(7), 1-15. https://doi.org/10.1371/journal.pone.0159742

A new imaging contrast agent is reported that provides an increased fluorescent signal upon application of ultrasound (US). Liposomes containing lipids labelled with pyrene were optically excited and the excimer fluorescence emission intensity was de... Read More about Ultrasound induced fluorescence of nanoscale liposome contrast agents.

On the combined effects of normobaric hypoxia and bed rest upon bone and mineral metabolism: Results from the PlanHab study (2016)
Journal Article
Rittweger, J., Debevec, T., Frings-Meuthen, P., Lau, P., Mittag, U., Ganse, B., …Mekjavic, I. B. (2016). On the combined effects of normobaric hypoxia and bed rest upon bone and mineral metabolism: Results from the PlanHab study. BONE, 91, 130-138. https://doi.org/10.1016/j.bone.2016.07.013

Bone losses are common as a consequence of unloading and also in patients with chronic obstructive pulmonary disease (COPD). Although hypoxia has been implicated as an important factor to drive bone loss, its interaction with unloading remains unreso... Read More about On the combined effects of normobaric hypoxia and bed rest upon bone and mineral metabolism: Results from the PlanHab study.

Adverse effects of anti-tuberculosis drugs on HepG2 cell bioenergetics (2016)
Journal Article
Elmorsy, E., Attalla, S., Fikry, E., Kocon, A., Turner, R., Christie, D., …Carter, W. (in press). Adverse effects of anti-tuberculosis drugs on HepG2 cell bioenergetics. Human and Experimental Toxicology, https://doi.org/10.1177/0960327116660751

Tuberculosis (TB) is an intractable chronic infection. Disease treatment with anti-TB drugs remains challenging due to drug-induced hepatotoxicity. The toxicity of the anti-TB drugs rifampicin (RIF), isoniazid (INH) and pyrazinamide (PZA) either alon... Read More about Adverse effects of anti-tuberculosis drugs on HepG2 cell bioenergetics.

Risk of venous thromboembolism in hospitalised cancer patients in England – A cohort study (2016)
Journal Article
Ratib, S., Walker, A. J., Card, T. R., & Grainge, M. J. (2016). Risk of venous thromboembolism in hospitalised cancer patients in England – A cohort study. Journal of Hematology and Oncology, 9, Article 60. https://doi.org/10.1186/s13045-016-0291-0

Background: Venous thromboembolism (VTE) is a well-recognised and life-threatening complication in patients with cancer. However, the precise risk of VTE in hospitalised cancer patients in England has not been previously reported. Methods: We condu... Read More about Risk of venous thromboembolism in hospitalised cancer patients in England – A cohort study.

Real-time selective sequencing using nanopore technology (2016)
Journal Article
Loose, M., Malla, S., & Stout, M. (in press). Real-time selective sequencing using nanopore technology. Nature Methods, 13(9), https://doi.org/10.1038/nmeth.3930

The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping t... Read More about Real-time selective sequencing using nanopore technology.

Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin (2016)
Journal Article
Scott, D., Garner, T. P., Long, J., Strachan, J., Mistry, S. C., Bottrill, A. R., …Layfield, R. (in press). Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin. Proteomics, 16(14), https://doi.org/10.1002/pmic.201600067

Unanchored polyubiquitin chains are emerging as importanregulators of cellular physiology with diverse roles paralleling those of substrate-conjugated polyubiquitin. However tools able to discriminate unanchored polyubiquitin chains of different isop... Read More about Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin.

Systematic and detailed analysis of behavioural tests in the rat middle cerebral artery occlusion model of stroke: Tests for long-term assessment (2016)
Journal Article
Trueman, R. C., Diaz, C., Farr, T. D., Harrison, D. J., Fuller, A., Tokarczuk, P. F., …Dunnett, S. B. (2017). Systematic and detailed analysis of behavioural tests in the rat middle cerebral artery occlusion model of stroke: Tests for long-term assessment. Journal of Cerebral Blood Flow and Metabolism, 37(4), 1349-1361. https://doi.org/10.1177/0271678X16654921

In order to test therapeutics, functional assessments are required. In pre-clinical stroke research, there is little consensus regarding the most appropriate behavioural tasks to assess deficits; especially when testing over extended times in milder... Read More about Systematic and detailed analysis of behavioural tests in the rat middle cerebral artery occlusion model of stroke: Tests for long-term assessment.

RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions (2016)
Journal Article
Miller, C. L., Romero, M., Karna, S. R., Chen, T., Heeb, S., & Leung, K. P. (2016). RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions. BMC Microbiology, 16, Article 155. https://doi.org/10.1186/s12866-016-0771-y

BACKGROUND: Biofilm development, specifically the fundamentally adaptive switch from acute to chronic infection phenotypes, requires global regulators and small non-coding regulatory RNAs (sRNAs). This work utilized RNA-sequencing (RNA-seq) to detect... Read More about RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions.

Immune dysfunction in patients with obstructive jaundice before and after endoscopic retrograde cholangiopancreatography (2016)
Journal Article
Chowdhury, A. H., Camara, M., Martinez-Pomares, L., Zaitoun, A. M., Eremin, O., Aithal, G. P., & Lobo, D. (2016). Immune dysfunction in patients with obstructive jaundice before and after endoscopic retrograde cholangiopancreatography. Clinical Science, 130(17), 1535-1544. https://doi.org/10.1042/CS20160326

© 2016 The Author(s). This prospective observational study investigated monocyte cytokine responses to lipopolysaccharide (LPS) in patients with obstructive jaundice (OJ) before and after endoscopic biliary drainage. Dendritic cell (DC) subsets and t... Read More about Immune dysfunction in patients with obstructive jaundice before and after endoscopic retrograde cholangiopancreatography.

A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis (2016)
Journal Article
Thambyrajah, R. S., Ucanok, D., Jalali, M., Hough, Y., Wilkinson, R. N., McMahon, K., …Gering, M. (2016). A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis. Developmental Biology, 417(1), 25-39. https://doi.org/10.1016/j.ydbio.2016.07.010

A transposon-mediated gene trap screen identified the zebrafish line qmc551 that expresses a GFP reporter in primitive erythrocytes and also in haemogenic endothelial cells, which give rise to haematopoietic stem and progenitor cells (HSPCs) that see... Read More about A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis.

Down-regulation of hippocampal genes regulating dopaminergic, GABAergic and glutamatergic function following combined neonatal phencyclidine and post-weaning social isolation of rats as a neurodevelopmental model for schizophrenia (2016)
Journal Article
Gaskin, P. L., Toledo-Rodriguez, M., Alexander, S. P., & Fone, K. C. (in press). Down-regulation of hippocampal genes regulating dopaminergic, GABAergic and glutamatergic function following combined neonatal phencyclidine and post-weaning social isolation of rats as a neurodevelopmental model for schizophrenia. International Journal of Neuropsychopharmacology, https://doi.org/10.1093/ijnp/pyw062

Background: Dysfunction of dopaminergic, GABAergic and glutamatergic function underlies many core symptoms of schizophrenia. Combined neonatal injection of the N-methyl-D-aspartate (NMDA) receptor antagonist, phencyclidine (PCP) and post-weaning soci... Read More about Down-regulation of hippocampal genes regulating dopaminergic, GABAergic and glutamatergic function following combined neonatal phencyclidine and post-weaning social isolation of rats as a neurodevelopmental model for schizophrenia.

The von Hippel-Lindau Chuvash mutation in mice alters cardiac substrate and high energy phosphate metabolism (2016)
Journal Article
Slingo, M., Cole, M., Carr, C., Curtis, M. K., Dodd, M., Giles, L., …Robbins, P. A. (in press). The von Hippel-Lindau Chuvash mutation in mice alters cardiac substrate and high energy phosphate metabolism. AJP - Heart and Circulatory Physiology, https://doi.org/10.1152/ajpheart.00912.2015

Hypoxia-inducible factor (HIF) appears to function as a global master regulator of cellular and systemic responses to hypoxia. HIF-pathway manipulation is of therapeutic interest, however global, systemic upregulation of HIF may have as yet unknown e... Read More about The von Hippel-Lindau Chuvash mutation in mice alters cardiac substrate and high energy phosphate metabolism.

Dynamic immune cell recruitment after murine pulmonary Aspergillus fumigatus infection under different immunosuppressive regimens (2016)
Journal Article
Kalleda, N., Amich, J., Arslan, B., Poreddy, S., Mattenheimer, K., Mokhtari, Z., …Beilhack, A. (2016). Dynamic immune cell recruitment after murine pulmonary Aspergillus fumigatus infection under different immunosuppressive regimens. Frontiers in Microbiology, 7, https://doi.org/10.3389/fmicb.2016.01107

Humans are continuously exposed to airborne spores of the saprophytic fungus Aspergillus fumigatus. However, in healthy individuals pulmonary host defense mechanisms efficiently eliminate the fungus. In contrast, A. fumigatus causes devastating infec... Read More about Dynamic immune cell recruitment after murine pulmonary Aspergillus fumigatus infection under different immunosuppressive regimens.