All Outputs (161)

Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region (2024)
Journal Article
Mao, Y., Dewi, S. R., Harding, S. E., & Binner, E. (2024). Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region. Food Chemistry, 460, Article 140549. https://doi.org/10.1016/j.foodchem.2024.140549

This work investigated a sustainable and efficient approach of pectin extraction for banana peel waste valorisation and studied the influence of banana ripening stages (RS at 2,5 and 7). Although pectin content in banana peel raw material decreased d... Read More about Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region.

Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection (2024)
Journal Article
Betts, H. M., Luckett, J. C., & Hill, P. J. (2024). Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection. Molecular Imaging and Biology, https://doi.org/10.1007/s11307-024-01929-7

Purpose There is currently no ideal radiotracer for imaging bacterial infections. Radiolabelled d-amino acids are promising candidates because they are actively incorporated into the peptidoglycan of the bacterial cell wall, a structural feature whic... Read More about Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection.

Storability and Brewing Quality of Corn Grits: Impacts of Storage Temperature, Particle Size and Key Compositional Factors (2024)
Journal Article
Maia, C., & Cook, D. J. (2024). Storability and Brewing Quality of Corn Grits: Impacts of Storage Temperature, Particle Size and Key Compositional Factors. Journal of The American Society of Brewing Chemists, https://doi.org/10.1080/03610470.2024.2338609

Across international brewing operations corn grits from different suppliers vary substantially in terms of their physical properties and chemical composition. Present research aimed to highlight the key factors determining the brewing quality of stor... Read More about Storability and Brewing Quality of Corn Grits: Impacts of Storage Temperature, Particle Size and Key Compositional Factors.

The Potential of Plant-Produced Virus-like Particle Vaccines for African Horse Sickness and Other Equine Orbiviruses (2024)
Journal Article
Pitchers, K. G., Boakye, O. D., Campeotto, I., & Daly, J. M. (2024). The Potential of Plant-Produced Virus-like Particle Vaccines for African Horse Sickness and Other Equine Orbiviruses. Pathogens, 13(6), Article 458. https://doi.org/10.3390/pathogens13060458

African horse sickness is a devastating viral disease of equids. It is transmitted by biting midges of the genus Culicoides with mortalities reaching over 90% in naïve horses. It is endemic to sub-Saharan Africa and is seasonally endemic in many part... Read More about The Potential of Plant-Produced Virus-like Particle Vaccines for African Horse Sickness and Other Equine Orbiviruses.

Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution (2024)
Journal Article
Ward, S., Childs, A., Staley, C., Waugh, C., Watts, J. A., Kotowska, A. M., …Borkar, A. N. (2024). Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution. Nature Communications, 15(1), Article 4367. https://doi.org/10.1038/s41467-024-48694-3

The 3D architecture of RNAs governs their molecular interactions, chemical reactions, and biological functions. However, a large number of RNAs and their protein complexes remain poorly understood due to the limitations of conventional structural bio... Read More about Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution.

Optimisation of Saccharomyces cerevisiae BRYC 501 ascospore formation and recovery for heat inactivation experiments (2024)
Journal Article
Raleigh, C., Lawrence, S., & Rachon, G. (2024). Optimisation of Saccharomyces cerevisiae BRYC 501 ascospore formation and recovery for heat inactivation experiments. Journal of the Institute of Brewing, 130(2), 70-82. https://doi.org/10.58430/jib.v130i2.48

Why was the work done: Ascospores from Saccharomyces cerevisiae BRYC 501 are useful biological indicators for validating the pasteurisation of beer. Ascospores are formed by yeast in response to low nutrient conditions and are characterised by greate... Read More about Optimisation of Saccharomyces cerevisiae BRYC 501 ascospore formation and recovery for heat inactivation experiments.

Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct (2024)
Journal Article
Yorke, J., Dew, T., & Cook, D. (2024). Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct. Journal of the Institute of Brewing, 130(1), 31-46. https://doi.org/10.58430/jib.v130i1.46

Why was the work done: The incorporation of unmalted barley at high proportion in the grist can introduce unfavourable bitter and astringent characteristics to beer, resulting in an upper limit on the proportion used. The flavour active compounds fro... Read More about Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct.

The need for One Health systems-thinking approaches to understand multiscale dissemination of antimicrobial resistance (2024)
Journal Article
Arnold, K. E., Laing, G., McMahon, B. J., Fanning, S., Stekel, D. J., Pahl, O., …McIntyre, K. M. (2024). The need for One Health systems-thinking approaches to understand multiscale dissemination of antimicrobial resistance. The Lancet Planetary Health, 8(2), E124-E133. https://doi.org/10.1016/S2542-5196%2823%2900278-4

Although the effects of antimicrobial resistance (AMR) are most obvious at clinical treatment failure, AMR evolution, transmission, and dispersal happen largely in environmental settings, for example within farms, waterways, livestock, and wildlife.... Read More about The need for One Health systems-thinking approaches to understand multiscale dissemination of antimicrobial resistance.

The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies (2024)
Journal Article
Cook, R., Brown, N., Rihtman, B., Michniewski, S., Redgwell, T., Clokie, M., …Millard, A. (2024). The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies. Microbial Genomics, 10(2), Article 0.001198. https://doi.org/10.1099/mgen.0.001198

Viral metagenomics has fuelled a rapid change in our understanding of global viral diversity and ecology. Long-read sequencing and hybrid assembly approaches that combine long- and short-read technologies are now being widely implemented in bacterial... Read More about The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.

Development of an open-source carbon footprint calculator of the UK craft brewing value chain (2023)
Journal Article
Bowler, A. L., Rodgers, S., Meng, F., McKechnie, J., Cook, D. J., & Watson, N. J. (2024). Development of an open-source carbon footprint calculator of the UK craft brewing value chain. Journal of Cleaner Production, 435, Article 140181. https://doi.org/10.1016/j.jclepro.2023.140181

Craft breweries may fall behind large brewing companies in reducing the carbon footprints of their value chains due to limited resources, financial constraints, and a lack of technical knowledge to fully understand their emissions. However, by increa... Read More about Development of an open-source carbon footprint calculator of the UK craft brewing value chain.

Modeling of Space Crop-Based Dishes for Optimal Nutrient Delivery to Astronauts and Beyond on Earth (2023)
Journal Article
Liang, S., Rivera-Osorio, K., Burgess, A. J., Kumssa, D. B., Escribà-Gelonch, M., Fisk, I., …Hessel, V. (2024). Modeling of Space Crop-Based Dishes for Optimal Nutrient Delivery to Astronauts and Beyond on Earth. ACS Food Science & Technology, 4(1), 104–117. https://doi.org/10.1021/acsfoodscitech.3c00396

Future long-term human exploration of space will need a supply of resources for astronauts, including fresh food from space farms. This means it is necessary to identify combinations of crops that can be successfully grown together and which provide... Read More about Modeling of Space Crop-Based Dishes for Optimal Nutrient Delivery to Astronauts and Beyond on Earth.

Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh (2023)
Journal Article
Williams, A. D., Rousham, E., Neal, A. L., Amin, M. B., Hobman, J. L., Stekel, D., & Islam, M. A. (2023). Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh. Microbiology Spectrum, 11(6), e0176323. https://doi.org/10.1128/spectrum.01763-23

Interactions between humans, animals, and the environment are considered critical foci for addressing antimicrobial resistance (AMR). However, One Health data on AMR in low- and middle-income countries are presently scarce. Using metagenomics, we inv... Read More about Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh.

The adaptability of the ion binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF (2023)
Journal Article
Lithgo, R. M., Hanževački, M., Harris, G., Kamps, J. J. .. G., Holden, E., Gianga, T., …Scott, D. J. (2023). The adaptability of the ion binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF. Journal of Biological Chemistry, 299(11), Article 105331. https://doi.org/10.1016/j.jbc.2023.105331

The periplasmic chaperone SilF has been identified as part of an Ag(I) detoxification system in Gram negative bacteria. Sil proteins also bind Cu(I), but with reported weaker affinity, therefore leading to the designation of a specific detoxification... Read More about The adaptability of the ion binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF.

Combining isotope ratios for provenancing Viking Age iron artefacts in the British Isles: a pilot study (2023)
Journal Article
Harding, S. E., Jones, C., Evans, J., Milot, J., Cutajar, M., Bailey, E., …Pearce, M. (2023). Combining isotope ratios for provenancing Viking Age iron artefacts in the British Isles: a pilot study. RSC Advances, 13(44), 31292-31302. https://doi.org/10.1039/D3RA06367D

Stable and radiogenic isotope analysis – particularly using lead isotope analysis (LIA) - has previously been shown to be a useful tool for the provenancing of ancient metal artefacts of silver and copper and its alloys, but less progress has been ma... Read More about Combining isotope ratios for provenancing Viking Age iron artefacts in the British Isles: a pilot study.

A model of antibiotic resistance genes accumulation through lifetime exposure from food intake and antibiotic treatment (2023)
Journal Article
Todman, H., Arya, S., Baker, M., & Stekel, D. J. (2023). A model of antibiotic resistance genes accumulation through lifetime exposure from food intake and antibiotic treatment. PLoS ONE, 18(8), Article e0289941. https://doi.org/10.1371/journal.pone.0289941

Antimicrobial resistant bacterial infections represent one of the most serious contemporary global healthcare crises. Acquisition and spread of resistant infections can occur through community, hospitals, food, water or endogenous bacteria. Global ef... Read More about A model of antibiotic resistance genes accumulation through lifetime exposure from food intake and antibiotic treatment.

Quorum-sensing, intra- and inter-species competition in the staphylococci (2023)
Journal Article
Williams, P., Hill, P., Bonev, B., & Chan, W. C. (2023). Quorum-sensing, intra- and inter-species competition in the staphylococci. Microbiology, 169(8), https://doi.org/10.1099/mic.0.001381

In Gram-positive bacteria such as Staphylococcus aureus and the coagulase-negative staphylococci (CoNS), the accessory gene regulator (agr) is a highly conserved but polymorphic quorum-sensing system involved in colonization, virulence and biofilm de... Read More about Quorum-sensing, intra- and inter-species competition in the staphylococci.

Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin (2023)
Journal Article
Chun, T., Pattem, J., Gillis, R. B., Dinu, V. T., Yakubov, G. E., Corfield, A. P., & Harding, S. E. (2023). Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin. Scientific Reports, 13, Article 11367. https://doi.org/10.1038/s41598-023-38036-6

Glycopeptide antibiotics are regularly used in ophthalmology to treat infections of Gram-positive bacteria. Aggregative interactions of antibiotics with mucins however can lead to long exposure and increases the risk of resistant species. This study... Read More about Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin.

Bayesian and ultrasonic sensor aided multi-objective optimisation for sustainable clean-in-place processes (2023)
Journal Article
Bowler, A. L., Rodgers, S., Cook, D. J., & Watson, N. J. (2023). Bayesian and ultrasonic sensor aided multi-objective optimisation for sustainable clean-in-place processes. Food and Bioproducts Processing, 141, 23-35. https://doi.org/10.1016/j.fbp.2023.06.010

In food and drink manufacturing, clean-in-place procedures are essential for hygienic and efficient operations but often over-clean process equipment leading to unnecessary use of energy, water, and chemicals. Previous attempts in the literature to o... Read More about Bayesian and ultrasonic sensor aided multi-objective optimisation for sustainable clean-in-place processes.

Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships (2023)
Journal Article
Myers, B., Catrambone, F., Allen, S., Hill, P. J., Kovacs, K., & Rawson, F. J. (2023). Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships. Scientific Reports, 13(1), Article 8843. https://doi.org/10.1038/s41598-023-35553-2

Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming. Their role is thought to be linked to facilitating electron transfer between cells and the external... Read More about Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships.

Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics (2023)
Journal Article
Chio, H., Guest, E. E., Hobman, J. L., Dottorini, T., Hirst, J. D., & Stekel, D. J. (2023). Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics. Journal of Molecular Graphics and Modelling, 123, Article 108508. https://doi.org/10.1016/j.jmgm.2023.108508

Antibiotics enter the environment through waste streams, where they can exert selective pressure for antimicrobial resistance in bacteria. However, many antibiotics are excreted as partly metabolized forms, or can be subject to partial breakdown in w... Read More about Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics.