All Outputs (7)

Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis (2024)
Journal Article
Cleary, S. E., Hall, S. J., Galan-Bataller, R., Lurshay, T. C., Hancox, C., Williamson, J. J., Heap, J. T., Reeve, H. A., & Morra, S. (2024). Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis. ChemCatChem, 16(16), Article e202400193. https://doi.org/10.1002/cctc.202400193

The enzyme CbA5H, a [FeFe]-hydrogenase from Clostridium beijerinckii, has previously been shown to survive exposure to oxygen, making it a promising candidate for biotechnological applications. Thus far [NiFe]-hydrogenases are typically considered fo... Read More about Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis.

Oxidation of cadaverine by putrescine oxidase from Rhodococcus erythropolis (2021)
Journal Article
Anyanwu, V. E., Hall, S. J., Stephens, G., & Pordea, A. (2021). Oxidation of cadaverine by putrescine oxidase from Rhodococcus erythropolis. Journal of Chemical Technology and Biotechnology, 96(10), 2950-2955. https://doi.org/10.1002/jctb.6851

BACKGROUND
Putrescine oxidase (EC 1.4.3.10) is of interest for the microbial production of unsubstituted platform nitrogen (N-)heterocycles, because it only requires inexpensive oxygen as co-substrate. Putrescine oxidase from Rhodococcus erythropoli... Read More about Oxidation of cadaverine by putrescine oxidase from Rhodococcus erythropolis.

Efficient bio-production of citramalate using an engineered Escherichia coli strain (2017)
Journal Article
Webb, J. P., Arnold, S. A., Baxter, S., Hall, S. J., Eastham, G., & Stephens, G. (2018). Efficient bio-production of citramalate using an engineered Escherichia coli strain. Microbiology, 164(2), 133-141. https://doi.org/10.1099/mic.0.000581

Citramalic acid is a central intermediate in a combined biocatalytic and chemocatalytic route to produce bio-based methylmethacrylate, the monomer used to manufacture Perspex and other high performance materials. We developed an engineered E. coli st... Read More about Efficient bio-production of citramalate using an engineered Escherichia coli strain.

Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes (2017)
Journal Article
Mordaka, P. M., Hall, S. J., Minton, N. P., & Stephens, G. (in press). Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes. Microbiology, https://doi.org/10.1099/mic.0.000568

‘Ene’-reductases have attracted significant attention for the preparation of chemical intermediates and biologically active products. To date, research has been focussed primarily on Old Yellow Enzyme-like proteins, due to their ease of handling, whe... Read More about Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes.

An automated fabrication strategy to create patterned tubular architectures at cell and tissue scales (2015)
Journal Article
Othman, R., Morris, G. E., Shah, D. A., Hall, S., Hall, G., Wells, K., Shakesheff, K. M., & Dixon, J. E. (2015). An automated fabrication strategy to create patterned tubular architectures at cell and tissue scales. Biofabrication, 7(2), Article 025003. https://doi.org/10.1088/1758-5090/7/2/025003

The putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene (2015)
Journal Article
Rossoni, L., Hall, S. J., Eastham, G., Licence, P., & Stephens, G. (2015). The putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene. Applied and Environmental Microbiology, 81(7), https://doi.org/10.1128/AEM.04033-14

Mevalonate diphosphate decarboxylase (MVD) is an ATP-dependent enzyme that catalyzes the phosphorylation/decarboxylation of (R)-mevalonate-5-diphosphate to isopentenyl pyrophosphate in the mevalonate (MVA) pathway.MVD is a key enzyme in engineered me... Read More about The putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene.

Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation (2014)
Journal Article
Dixon, J. E., Shah, D. A., Rogers, C., Hall, S., Weston, N., Parmenter, C. D., McNally, D., Denning, C., & Shakesheff, K. M. (2014). Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. Proceedings of the National Academy of Sciences, 111(15), 5580-5585. https://doi.org/10.1073/pnas.1319685111

The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the... Read More about Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation.