All Outputs (22)

The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module (2015)
Journal Article
Maryati, M., Airhihen, B., & Winkler, G. S. (2015). The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module. Biochemical Journal, 469(1), https://doi.org/10.1042/BJ20150304

In eukaryotic cells, the shortening and removal of the poly(A) tail (deadenylation) of cytoplasmic mRNA is a key event in regulated mRNA degradation. A major enzyme involved in deadenylation is the Ccr4-Not deadenylase complex, which can be recruited... Read More about The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module.

Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag (2014)
Journal Article
Hage, N., Falcone, F., Renshaw, J. G., Sebastiaan Winkler, G., Gellert, P., & Stolnik, S. (2015). Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag. Protein Expression and Purification, 106, 25-30. https://doi.org/10.1016/j.pep.2014.10.009

Helicobacter pylori is a pathogenic bacterium that has the remarkable ability to withstand the harsh conditions of the stomach for decades. This is achieved through unique evolutionary adaptations, which include binding Lewisb antigens found on the g... Read More about Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag.