All Outputs (2)

Targeted DNA transposition in vitro using a dCas9-transposase fusion protein (2019)
Journal Article
Bhatt, S., & Chalmers, R. (2019). Targeted DNA transposition in vitro using a dCas9-transposase fusion protein. Nucleic Acids Research, 47(15), 8126–8135. https://doi.org/10.1093/nar/gkz552

Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates effic... Read More about Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.

The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair (2019)
Journal Article
Tellier, M., & Chalmers, R. (2019). The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair. DNA Repair, 80, 26-35. https://doi.org/10.1016/j.dnarep.2019.06.006

SETMAR is a fusion between a SET-domain methyltransferase gene and a mariner-family transposase gene, which is specific to anthropoid primates. However, the ancestral SET gene is present in all other mammals and birds. SETMAR is reported to be involv... Read More about The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair.