Skip to main content

Research Repository

Advanced Search

All Outputs (9)

The phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, 3-mercaptopicolinic acid (3-MPA), induces myogenic differentiation in C2C12 cells (2020)
Journal Article
Brearley, M. C., Daniel, Z. C., Loughna, P. T., Parr, T., & Brameld, J. M. (2020). The phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, 3-mercaptopicolinic acid (3-MPA), induces myogenic differentiation in C2C12 cells. Scientific Reports, 10, Article 22177. https://doi.org/10.1038/s41598-020-79324-9

Phosphoenolpyruvate carboxykinase (PEPCK) is a gluconeogenic enzyme with a cytosolic (Pck1/PEPCK-C) and mitochondrial (Pck2/PEPCK-M) isoform. Here we investigate the effect of 3-mercaptopicolinic acid (3-MPA), a PEPCK inhibitor, on C2C12 muscle cells... Read More about The phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, 3-mercaptopicolinic acid (3-MPA), induces myogenic differentiation in C2C12 cells.

Changes in expression of serine biosynthesis and integrated stress response genes during myogenic differentiation of C2C12 cells (2019)
Journal Article
Brearley, M. C., Li, C., Daniel, Z. C., Loughna, P. T., Parr, T., & Brameld, J. M. (2019). Changes in expression of serine biosynthesis and integrated stress response genes during myogenic differentiation of C2C12 cells. Biochemistry and Biophysics Reports, 20, Article 100694. https://doi.org/10.1016/j.bbrep.2019.100694

Skeletal muscle is a highly metabolic and dynamic tissue that is formed through the complex and well-organised process of myogenesis. Although there is a good understanding about the role of the Muscle Regulatory Factors during myogenesis, little is... Read More about Changes in expression of serine biosynthesis and integrated stress response genes during myogenic differentiation of C2C12 cells.

Adipogenic differentiation of muscle derived cells is repressed by inhibition of GSK-3 activity (2018)
Journal Article
Redshaw, Z., & Loughna, P. T. (2018). Adipogenic differentiation of muscle derived cells is repressed by inhibition of GSK-3 activity. Frontiers in Veterinary Science, 5, Article 110. https://doi.org/10.3389/fvets.2018.00110

Background: Intramuscular fat is important in large animal livestock species in regard to meat quality and in humans is of clinical significance in particular in relation to insulin resistance. The canonical Wnt signalling pathway has been implicated... Read More about Adipogenic differentiation of muscle derived cells is repressed by inhibition of GSK-3 activity.

The effects of age upon the expression of three miRNAs in muscle stem cells isolated from two different porcine skeletal muscles (2014)
Journal Article
Redshaw, Z., Sweetman, D., & Loughna, P. (in press). The effects of age upon the expression of three miRNAs in muscle stem cells isolated from two different porcine skeletal muscles. Differentiation, 88(4-5), https://doi.org/10.1016/j.diff.2014.12.001

Aging is associated with a gradual loss of skeletal muscle mass and an impaired ability of this tissue to compensate for trauma. Studies in rodents and humans have also shown that resident stem cells within muscle have a reduced ability to proliferat... Read More about The effects of age upon the expression of three miRNAs in muscle stem cells isolated from two different porcine skeletal muscles.

Influence of 1?, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes (2014)
Journal Article
Hdud, I. M., & Loughna, P. (2014). Influence of 1?, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes. Journal of Animal Science and Technology, 56, Article 33. https://doi.org/10.1186/s40781-014-0033-1

Background Sox 9 is a major marker of chondrocyte differentiation. When chondrocytes are cultured in vitro they progressively de-differentiate and this is associated with a decline in Sox 9 expression. The active form of vitamin D, 1, 25 (OH)2D3... Read More about Influence of 1?, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes.

Effect of osmotic stress on the expression of TRPV4 and BKCa channels and possible interaction with ERK1/2 and p38 in cultured equine chondrocytes (2014)
Journal Article
Hdud, I. M., Mobasheri, A., & Loughna, P. (2014). Effect of osmotic stress on the expression of TRPV4 and BKCa channels and possible interaction with ERK1/2 and p38 in cultured equine chondrocytes. American Journal of Physiology - Cell Physiology, 306(11), https://doi.org/10.1152/ajpcell.00287.2013

The metabolic activity of articular chondrocytes is influenced by osmotic alterations that occur in articular cartilage secondary to mechanical load. The mechanisms that sense and transduce mechanical signals from cell swelling and initiate volume re... Read More about Effect of osmotic stress on the expression of TRPV4 and BKCa channels and possible interaction with ERK1/2 and p38 in cultured equine chondrocytes.

Effects of cyclic equibiaxial mechanical stretch on ?-BK and TRPV4 expression in equine chondrocytes (2014)
Journal Article
Hdud, I. M., Mobasheri, A., & Loughna, P. (2014). Effects of cyclic equibiaxial mechanical stretch on ?-BK and TRPV4 expression in equine chondrocytes. SpringerPlus, 3, Article 59. https://doi.org/10.1186/2193-1801-3-59

Background Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The pu... Read More about Effects of cyclic equibiaxial mechanical stretch on ?-BK and TRPV4 expression in equine chondrocytes.

Oxygen concentration modulates the differentiation of muscle stem cells toward myogenic and adipogenic fates (2012)
Journal Article
Redshaw, Z., & Loughna, P. T. (2012). Oxygen concentration modulates the differentiation of muscle stem cells toward myogenic and adipogenic fates. Differentiation, 84(2), https://doi.org/10.1016/j.diff.2012.06.001

The physiological oxygen concentration of many tissues is far lower than that in which cells are typically cultured in vitro and this may inadvertently influence the proliferation and differentiation potential of many cell types. Muscle derived stem... Read More about Oxygen concentration modulates the differentiation of muscle stem cells toward myogenic and adipogenic fates.

Expression of transient receptor potential vanilloid (TRPV) channels in different passages of articular chondrocytes (2012)
Journal Article
Hdud, I. M., El-Shafei, A. A., Loughna, P., Barrett-Jolley, R., & Mobasheri, A. (2012). Expression of transient receptor potential vanilloid (TRPV) channels in different passages of articular chondrocytes. International Journal of Molecular Sciences, 13(4), https://doi.org/10.3390/ijms13044433

Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV) subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective c... Read More about Expression of transient receptor potential vanilloid (TRPV) channels in different passages of articular chondrocytes.