All Outputs (11)

Peeking into the future: inferring mechanics in dynamical tissues (2024)
Journal Article
Borges, A., & Chara, O. (2024). Peeking into the future: inferring mechanics in dynamical tissues. Biochemical Society Transactions, 52(6), 2579-2592. https://doi.org/10.1042/BST20230225

Cells exert forces on each other and their environment, shaping the tissue. The resulting mechanical stresses can be determined experimentally or estimated computationally using stress inference methods. Over the years, mechanical stress inference ha... Read More about Peeking into the future: inferring mechanics in dynamical tissues.

Lineage tracing of Shh+ floor plate cells and dynamics of dorsal–ventral gene expression in the regenerating axolotl spinal cord (2024)
Journal Article
Arbanas, L. I., Cura Costa, E., Chara, O., Otsuki, L., & Tanaka, E. M. (2024). Lineage tracing of Shh+ floor plate cells and dynamics of dorsal–ventral gene expression in the regenerating axolotl spinal cord. Development, Growth and Differentiation, 66(8), 414-425. https://doi.org/10.1111/dgd.12945

Both development and regeneration depend on signaling centers, which are sources of locally secreted tissue-patterning molecules. As many signaling centers are decommissioned before the end of embryogenesis, a fundamental question is how signaling ce... Read More about Lineage tracing of Shh+ floor plate cells and dynamics of dorsal–ventral gene expression in the regenerating axolotl spinal cord.

How a reaction-diffusion signal can control spinal cord regeneration in axolotls: A modeling study. (2024)
Journal Article
Caliaro, V., Peurichard, D., & Chara, O. (2024). How a reaction-diffusion signal can control spinal cord regeneration in axolotls: A modeling study. iScience, 27(7), Article 110197. https://doi.org/10.1016/j.isci.2024.110197

Axolotls are uniquely able to completely regenerate the spinal cord after amputation. The underlying governing mechanisms of this regenerative response have not yet been fully elucidated. We previously found that spinal cord regeneration is mainly dr... Read More about How a reaction-diffusion signal can control spinal cord regeneration in axolotls: A modeling study..

Fluctuations in tissue growth portray homeostasis as a critical state and long-time non-Markovian cell proliferation as Markovian (2023)
Journal Article
Lavalle, N. G., Chara, O., & Grigera, T. S. (2023). Fluctuations in tissue growth portray homeostasis as a critical state and long-time non-Markovian cell proliferation as Markovian. Royal Society Open Science, 10(9), Article 230871. https://doi.org/10.1098/rsos.230871

Tissue growth is an emerging phenomenon that results from the cell-level interplay between proliferation and apoptosis, which is crucial during embryonic development, tissue regeneration, as well as in pathological conditions such as cancer. In this... Read More about Fluctuations in tissue growth portray homeostasis as a critical state and long-time non-Markovian cell proliferation as Markovian.

Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo (2023)
Journal Article
Kozak, E. L., Miranda-Rodríguez, J. R., Borges, A., Dierkes, K., Mineo, A., Pinto-Teixeira, F., Viader-Llargués, O., Solon, J., Chara, O., & López-Schier, H. (2023). Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo. Development, 150(9), Article dev200975. https://doi.org/10.1242/dev.200975

Collective cell rotations are widely used during animal organogenesis. Theoretical and in vitro studies have conceptualized rotating cells as identical rigid-point objects that stochastically break symmetry to move monotonously and perpetually within... Read More about Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo.

Vectorcardiography-derived index allows a robust quantification of ventricular electrical synchrony (2022)
Journal Article
Fernández, J. M. F., Spagnuolo, D. N., Politi, M. T., Tello Santacruz, I. A., Schiavone, M., Cáceres Monié, C., Avaca, H. A., & Chara, O. (2022). Vectorcardiography-derived index allows a robust quantification of ventricular electrical synchrony. Scientific Reports, 12(1), Article 9961. https://doi.org/10.1038/s41598-022-14000-8

Alteration of muscle activation sequence is a key mechanism in heart failure with reduced ejection fraction. Successful cardiac resynchronization therapy (CRT), which has become standard therapy in these patients, is limited by the lack of precise dy... Read More about Vectorcardiography-derived index allows a robust quantification of ventricular electrical synchrony.

Tig1 regulates proximo-distal identity during salamander limb regeneration (2022)
Journal Article
Oliveira, C. R., Knapp, D., Elewa, A., Gerber, T., Gonzalez Malagon, S. G., Gates, P. B., Walters, H. E., Petzold, A., Arce, H., Cordoba, R. C., Subramanian, E., Chara, O., Tanaka, E. M., Simon, A., & Yun, M. H. (2022). Tig1 regulates proximo-distal identity during salamander limb regeneration. Nature Communications, 13(1), Article 1141. https://doi.org/10.1038/s41467-022-28755-1

Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed pos... Read More about Tig1 regulates proximo-distal identity during salamander limb regeneration.

Photosensitivity and cGAS-Dependent IFN-1 Activation in Patients with Lupus and TREX1 Deficiency (2022)
Journal Article
Berndt, N., Wolf, C., Fischer, K., Cura Costa, E., Knuschke, P., Zimmermann, N., Schmidt, F., Merkel, M., Chara, O., Lee-Kirsch, M. A., & Günther, C. (2022). Photosensitivity and cGAS-Dependent IFN-1 Activation in Patients with Lupus and TREX1 Deficiency. Journal of Investigative Dermatology, 142(3), 633-640.e6. https://doi.org/10.1016/j.jid.2021.04.037

The exonuclease TREX1 safeguards the cells against DNA accumulation in the cytosol and thereby prevents innate immune activation and autoimmunity. TREX1 mutations lead to chronic DNA damage and cell-intrinsic IFN-1 response. Associated disease phenot... Read More about Photosensitivity and cGAS-Dependent IFN-1 Activation in Patients with Lupus and TREX1 Deficiency.

Size matters: tissue size as a marker for a transition between reaction–diffusion regimes in spatio-temporal distribution of morphogens (2022)
Journal Article
Ceccarelli, A. S., Borges, A., & Chara, O. (2022). Size matters: tissue size as a marker for a transition between reaction–diffusion regimes in spatio-temporal distribution of morphogens. Royal Society Open Science, 9(1), Article 211112. https://doi.org/10.1098/rsos.211112

The reaction–diffusion model constitutes one of the most influential mathematical models to study distribution of morphogens in tissues. Despite its widespread use, the effect of finite tissue size on model-predicted spatio-temporal morphogen distrib... Read More about Size matters: tissue size as a marker for a transition between reaction–diffusion regimes in spatio-temporal distribution of morphogens.

Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum (2021)
Journal Article
Riquelme‐Guzmán, C., Schuez, M., Böhm, A., Knapp, D., Edwards‐Jorquera, S., Ceccarelli, A. S., Chara, O., Rauner, M., & Sandoval‐Guzmán, T. (2022). Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum. Developmental Dynamics, 251(6), 1015-1034. https://doi.org/10.1002/dvdy.407

Background
The axolotl is a key model to study appendicular regeneration. The limb complexity resembles that of humans in structure and tissue components; however, axolotl limbs develop postembryonically. In this work, we evaluated the postembryonic... Read More about Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum.

Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration (2021)
Journal Article
Costa, E. C., Otsuki, L., Albors, A. R., Tanaka, E. M., & Chara, O. (2021). Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration. eLife, 10, Article e55665. https://doi.org/10.7554/eLife.55665

Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ep... Read More about Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration.