Skip to main content

Research Repository

See what's under the surface


Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers (2020)
Journal Article
Ogden, A., Bhattarai, S., Sahoo, B., Mongan, N. P., Alsaleem, M., Green, A. R., …Aneja, R. (2020). Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers. Scientific Reports, 10, https://doi.org/10.1038/s41598-020-59514-1

Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 3 (HER3) have been investigated as triple-negative breast cancer (TNBC) biomarkers. Reduced EGFR levels can be compensated by increases in HER3; thus, assaying EGFR an... Read More about Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers.

All-trans retinoic acid (ATRA) induced TFEB expression is required for myeloid differentiation in acute promyelocytic leukemia (APL) (2020)
Journal Article
Orfali, N., O'Donovan, T. R., Cahill, M. R., Benjamin, D., Nanus, D. M., McKenna, S. L., …Mongan, N. (2020). All-trans retinoic acid (ATRA) induced TFEB expression is required for myeloid differentiation in acute promyelocytic leukemia (APL). European Journal of Haematology, https://doi.org/10.1111/ejh.13367

Objective In acute promyelocytic leukemia (APL), normal retinoid signaling is disrupted by an abnormal PML-RARα fusion oncoprotein, leading to a block in cell differentiation. Therapeutic concentrations of all-trans-retinoic acid (ATRA) can restore... Read More about All-trans retinoic acid (ATRA) induced TFEB expression is required for myeloid differentiation in acute promyelocytic leukemia (APL).

Prognostic Significance of KN Motif and Ankyrin Repeat Domains 1 (KANK1) in Invasive Breast Cancer (2019)
Journal Article
Kariri, Y. A., Joseph, C., Kurozumi, S., Toss, M. S., Alsaleem, M., Raafat, S., …Rakha, E. A. (2020). Prognostic Significance of KN Motif and Ankyrin Repeat Domains 1 (KANK1) in Invasive Breast Cancer. Breast Cancer Research and Treatment, 179(2), 349–357. https://doi.org/10.1007/s10549-019-05466-8

Background: KN Motif and Ankyrin Repeat Domains 1 (KANK1) plays an important role in cytoskeleton maintenance and contributes to the regulation of cell proliferation, adhesion and apoptosis. KANK1 is involved in progression of a variety of solid tumo... Read More about Prognostic Significance of KN Motif and Ankyrin Repeat Domains 1 (KANK1) in Invasive Breast Cancer.

The prognostic significance of wild type isocitrate dehydrogenase 2 (IDH2) in breast cancer (2019)
Journal Article
Aljohani, A. I., Toss, M. S., Kurozumi, S., Joseph, C., Aleskandarany, M. A., Miligy, I. M., …Rakha, E. (2020). The prognostic significance of wild type isocitrate dehydrogenase 2 (IDH2) in breast cancer. Breast Cancer Research and Treatment, 179(1), 79–90. https://doi.org/10.1007/s10549-019-05459-7

Background: Lymphovascular invasion (LVI) is a prerequisite step in breast cancer (BC) metastasis. We have previously identified wild type isocitrate dehydrogenase 2 (IDH2) as a key putative driver of LVI. Thus, we explored the prognostic significanc... Read More about The prognostic significance of wild type isocitrate dehydrogenase 2 (IDH2) in breast cancer.

Retinoid X receptor gamma (RXRG) is an independent prognostic biomarker in ER-positive invasive breast cancer (2019)
Journal Article
Joseph, C., Al-Izzi, S., Alsaleem, M., Kurozumi, S., Toss, M., Arshad, M., …Rakha, E. A. (2019). Retinoid X receptor gamma (RXRG) is an independent prognostic biomarker in ER-positive invasive breast cancer. British Journal of Cancer, 121(9), 776-785. https://doi.org/10.1038/s41416-019-0589-0

© 2019, The Author(s), under exclusive licence to Cancer Research UK. Background: Retinoid X Receptor Gamma (RXRG) is a member of the nuclear receptor superfamily and plays a role in tumour suppression. This study aims to explore the prognostic signi... Read More about Retinoid X receptor gamma (RXRG) is an independent prognostic biomarker in ER-positive invasive breast cancer.

Lentiviral-Mediated shRNA Approaches: Applications in Cellular Differentiation and Autophagy (2019)
Book Chapter
Orfali, N., Jeyapalan, J. N., Woodcock, C. L., O’Donovan, T. R., Benjamin, D., Cahill, M., …Mongan, N. P. (2019). Lentiviral-Mediated shRNA Approaches: Applications in Cellular Differentiation and Autophagy. In Retinoid and Rexinoid Signaling, (33-49). New York: Humana Press. https://doi.org/10.1007/978-1-4939-9585-1_3

Acute myeloid leukemia (AML) is characterized by the accumulation of immature white blood cell precursors in the bone marrow and peripheral circulation. In essence, leukemic cells fail to differentiate and are stalled at a particular step of hematopo... Read More about Lentiviral-Mediated shRNA Approaches: Applications in Cellular Differentiation and Autophagy.

A Key Genomic Subtype Associated with Lymphovascular Invasion in Invasive Breast Cancer (2019)
Journal Article
Kurozumi, S., Joseph, C., Alsaeed, S., Kariri, Y., Aljohani, A., Raafat, S., …Rakha, E. A. (2019). A Key Genomic Subtype Associated with Lymphovascular Invasion in Invasive Breast Cancer. British Journal of Cancer, 120, 1129–1136. doi:10.1038/s41416-019-0486-6

Background: Lymphovascular invasion (LVI) is associated with the development of metastasis in invasive breast cancer (BC). However, the complex molecular mechanisms of LVI, which overlap with other oncogenic pathways, remain unclear. This study, usin... Read More about A Key Genomic Subtype Associated with Lymphovascular Invasion in Invasive Breast Cancer.

A Fibromyxoid Stromal Response is Associated with Muscle Invasion in Canine Urothelial Carcinoma (2019)
Journal Article
de Brot, S., Grau-Roma, L., Stirling-Stainsby, C., Dettwiler, M., Guscetti, F., Meier, D., …Mongan, N. (2019). A Fibromyxoid Stromal Response is Associated with Muscle Invasion in Canine Urothelial Carcinoma. Journal of Comparative Pathology, 169, 35-46. doi:10.1016/j.jcpa.2019.04.003

Canine urothelial carcinoma (UC) is the most common type of cancer of the lower urinary tract and tends to affect elderly neutered female dogs, with a high predisposition for Scottish terriers. Tumour stroma, inflammation and necrosis are poorly char... Read More about A Fibromyxoid Stromal Response is Associated with Muscle Invasion in Canine Urothelial Carcinoma.

Metadherin: A Therapeutic Target in Multiple Cancers (2019)
Journal Article
Dhiman, G., Srivastava, N., Goyal, M., Rakha, E., Lothion-Roy, J., Mongan, N. P., …Baranwal, M. (2019). Metadherin: A Therapeutic Target in Multiple Cancers. Frontiers in Oncology, 9, doi:10.3389/fonc.2019.00349

Altered expression of many genes and proteins is essential for cancer development and progression. Recently, the affected expression of metadherin (MTDH), also known as AEG-1 (Astrocyte Elevated Gene 1) and Lyric, has been implicated in various aspec... Read More about Metadherin: A Therapeutic Target in Multiple Cancers.

Detection and analysis of RNA methylation (2019)
Journal Article
Mongan, N. P., Emes, R. D., & Archer, N. (2019). Detection and analysis of RNA methylation. F1000Research, 8, 559. doi:10.12688/f1000research.17956.1

The molecular mechanisms underlying reduced E-cadherin expression in invasive ductal carcinoma of the breast: high throughput analysis of large cohorts (2019)
Journal Article
Rida, P. C. G., Alsaleem, M., Toss, M. S., Joseph, C., Aleskandarany, M., Kurozumi, S., …Rakha, E. A. (2019). The molecular mechanisms underlying reduced E-cadherin expression in invasive ductal carcinoma of the breast: high throughput analysis of large cohorts. Modern Pathology, doi:10.1038/s41379-019-0209-9

E-cadherin is a tumor suppressor gene in invasive lobular breast cancer. However, a proportion of high-grade ductal carcinoma shows reduced/loss of E-cadherin. In this study, we assessed the underlying mechanisms and mole... Read More about The molecular mechanisms underlying reduced E-cadherin expression in invasive ductal carcinoma of the breast: high throughput analysis of large cohorts.

Overexpression of the cancer stem cell marker CD133 confers a poor prognosis in invasive breast cancer (2018)
Journal Article
Joseph, C., Arshad, M., Kurozomi, S., Althobiti, M., Miligy, I. M., Al-Izzi, S., …Rakha, E. A. (2019). Overexpression of the cancer stem cell marker CD133 confers a poor prognosis in invasive breast cancer. Breast Cancer Research and Treatment, 174(2), 387-399. doi:10.1007/s10549-018-05085-9

Purpose CD133/ prominin 1 is a cancer stem cell marker associated with cancer progression and patient outcome in a variety of solid tumours, but its role in invasive breast cancer (BC) remains obscure. The current study aims to assess the prognostic... Read More about Overexpression of the cancer stem cell marker CD133 confers a poor prognosis in invasive breast cancer.

CARM1 (PRMT4) acts as a transcriptional coactivator during retinoic acid-induced embryonic stem cell differentiation (2018)
Journal Article
Quintero, C. M., Laursen, K. B., Mongan, N. P., Luo, M., & Gudas, L. J. (2018). CARM1 (PRMT4) acts as a transcriptional coactivator during retinoic acid-induced embryonic stem cell differentiation. Journal of Molecular Biology, 430(21), 4168-4182. doi:10.1016/j.jmb.2018.08.014

Activation of the retinoic acid (RA) signaling pathway is important for controlling embryonic stem cell differentiation and development. Modulation of this pathway occurs through the recruitment of different epigenetic regulators at the retinoic acid... Read More about CARM1 (PRMT4) acts as a transcriptional coactivator during retinoic acid-induced embryonic stem cell differentiation.

The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor (2018)
Journal Article
Sarwar, M., Syed Khaja, A. S., Aleskandarany, M., Karlsson, R., Althobiti, M., Ødum, N., …Persson, J. L. (2018). The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor. Oncogene, doi:10.1038/s41388-018-0438-2

Despite recent improvement in adjuvant therapies, triple-negative, and ER+ subtypes of breast cancer (BC) with metastatic potentials remain the leading cause of BC-related deaths. We investigated the role of phosphatidylinositol-4-phosphate 5-kinase... Read More about The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.

Clinicopathological and prognostic significance of Ras association and pleckstrin homology domains 1 (RAPH1) in breast cancer (2018)
Journal Article
Kurozumi, S., Joseph, C., Sonbul, S., Aleskandarany, M. A., Pigera, M., Alsaleem, M., …Rakha, E. A. (2018). Clinicopathological and prognostic significance of Ras association and pleckstrin homology domains 1 (RAPH1) in breast cancer. Breast Cancer Research and Treatment, 172(1), 61–68. doi:10.1007/s10549-018-4891-y

BACKGROUND: Ras association and pleckstrin homology domains 1 (RAPH1) is involved in cytoskeleton regulation and re-epithelialisation in invasive carcinoma and therefore may play a key role in carcinogenesis and metastasis. We herein investigated the... Read More about Clinicopathological and prognostic significance of Ras association and pleckstrin homology domains 1 (RAPH1) in breast cancer.

Impact of breast cancer grade discordance on prediction of outcome (2018)
Journal Article
Rakha, E. A., Aleskandarany, M. A., Toss, M. S., Mongan, N., El-Sayed, M. E., Green, A. R., …Dalton, L. W. (in press). Impact of breast cancer grade discordance on prediction of outcome. Histopathology, doi:10.1111/his.13709

BACKGROUND: Histological grade is an independent prognostic variable in breast cancer (BC). Previous concordance studies of BC grade have reported moderate levels of agreement; a typical finding in morphological assessment of biological variables. Th... Read More about Impact of breast cancer grade discordance on prediction of outcome.

Heterogeneity of Tumour Infiltrating Lymphocytes (TILs) in breast cancer and its prognostic significance (2018)
Journal Article
Althobiti, M., Aleskandarany, M. A., Joseph, C., Toss, M., Mongan, N., Diez-Rodriguez, M., …Rakha, E. A. (2018). Heterogeneity of Tumour Infiltrating Lymphocytes (TILs) in breast cancer and its prognostic significance. Histopathology, 73(6), 887-896. doi:10.1111/his.13695

Background: Tumour-infiltrating lymphocytes (TILs) in breast cancer (BC) confer prognostic and predictive information. This study aims to assess the spatial and temporal heterogeneity of TILs in BC and its relationship with immune cell subtypes. Met... Read More about Heterogeneity of Tumour Infiltrating Lymphocytes (TILs) in breast cancer and its prognostic significance.