All Outputs (2)

Stable Platform for Mevalonate Bioproduction from CO2 (2024)
Journal Article
Garavaglia, M., McGregor, C., Bommareddy, R. R., Irorere, V., Arenas, C., Robazza, A., Minton, N. P., & Kovacs, K. (2024). Stable Platform for Mevalonate Bioproduction from CO2. ACS Sustainable Chemistry and Engineering, https://doi.org/10.1021/acssuschemeng.4c03561

Stable production of value-added products using a microbial chassis is pivotal for determining the industrial suitability of the engineered biocatalyst. Microbial cells often lose the multicopy expression plasmids during long-term cultivations. Owing... Read More about Stable Platform for Mevalonate Bioproduction from CO2.

Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production (2024)
Journal Article
Ehsaan, M., Yoo, M., Kuit, W., Foulquier, C., Soucaille, P., & Minton, N. P. (2024). Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production. Nature Microbiology, 9(7), 1655-1660. https://doi.org/10.1038/s41564-024-01714-w

Biofuel production by Clostridium acetobutylicum is compromised by strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway.... Read More about Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production.