All Outputs (2)

Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species (2021)
Journal Article
Kinoshita, M., Kobayashi, T., Planells, B., Klisch, D., Spindlow, D., Masaki, H., Bornelöv, S., Stirparo, G. G., Matsunari, H., Uchikura, A., Lamas-Toranzo, I., Nichols, J., Nakauchi, H., Nagashima, H., Alberio, R., & Smith, A. (2021). Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species. Development, 148(23), Article dev199901. https://doi.org/10.1242/dev.199901

Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the em... Read More about Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.

Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory (2021)
Journal Article
Kinoshita, M., Li, M. A., Barber, M., Mansfield, W., Dietmann, S., & Smith, A. (2021). Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory. Proceedings of the National Academy of Sciences, 118(38), Article e2109475118. https://doi.org/10.1073/pnas.2109475118

Genome remethylation is essential for mammalian development but specific reasons are unclear. Here we examined embryonic stem (ES) cell fate in the absence of de novo DNA methyltransferases. We observed that ES cells deficient for both Dnmt3a and Dnm... Read More about Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory.