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The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro (2022)
Journal Article
McCloskey, M. C., Kasap, P., Ahmad, S. D., Su, S., Chen, K., Mansouri, M., …McGrath, J. L. (2022). The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro. Advanced Healthcare Materials, 11(18), Article 2200804. https://doi.org/10.1002/adhm.202200804

Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support "on-chip" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow... Read More about The Modular μSiM: a Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro.

Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro (2020)
Journal Article
Harun, M., Marsh, V., Elsaied, N. A., Webb, K. F., & Elsheikha, H. M. (2020). Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro. Brain Research, 1746, Article 147002. https://doi.org/10.1016/j.brainres.2020.147002

Toxoplasma gondii can cause parasitic encephalitis, a life-threatening infection that predominately occurs in immunocompromised individuals. T. gondii has the ability to invade the brain, but the mechanisms by which this parasite crosses the blood–br... Read More about Effects of Toxoplasma gondii infection on the function and integrity of human cerebrovascular endothelial cells and the influence of Verapamil treatment in vitro.

Surface plasmon resonance imaging of excitable cells (2019)
Journal Article
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. (2019). Surface plasmon resonance imaging of excitable cells. Journal of Physics D: Applied Physics, 52(10), Article 104001. https://doi.org/10.1088/1361-6463/aaf849

Surface plasmons (SPs) are surface charge density oscillations occuring at a metal/dieletric interface and are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and... Read More about Surface plasmon resonance imaging of excitable cells.

TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN) (2018)
Journal Article
Madejski, G., Lucas, K., Pascut, F. C., Webb, K. F., & McGrath, J. L. (2018). TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN). Membranes, 8(2), Article 26. https://doi.org/10.3390/membranes8020026

Silicon nanomembrane technologies (NPN, pnc-Si, and others) have been used commercially as electron microscopy (EM) substrates, and as filters with nanometer-resolution size cut-offs. Combined with EM, these materials provide a platform for catching... Read More about TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN).

Rapid one-step biotinylation of biological and non-biological surfaces (2018)
Journal Article
Henry, S., Williams, E., Barr, K., Korchagina, E., Tuzikov, A., Ilyushina, N., …Bovin, N. (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8(1), Article 2845. https://doi.org/10.1038/s41598-018-21186-3

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minu... Read More about Rapid one-step biotinylation of biological and non-biological surfaces.

Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells (2018)
Journal Article
Petrova, R. S., Webb, K. F., Vaghefi, E., Walker, K., Schey, K. L., & Donaldson, P. J. (2018). Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells. American Journal of Physiology - Cell Physiology, 314(2), C191-C201. https://doi.org/10.1152/ajpcell.00214.2017

Although the functionality of the lens water channels aquaporin 1 (AQP1; epithelium) and AQP0 (fiber cells) is well established, less is known about the role of AQP5 in the lens. Since in other tissues AQP5 functions as a regulated water channel with... Read More about Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells.

Sensitive detection of voltage transients using differential intensity surface plasmon resonance system (2017)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2017). Sensitive detection of voltage transients using differential intensity surface plasmon resonance system. Optics Express, 25(25), 31552-31567. https://doi.org/10.1364/OE.25.031552

This paper describes theoretical and experimental study of the fundamentals of using surface plasmon resonance (SPR) for label-free detection of voltage. Plasmonic voltage sensing relies on the capacitive properties of metal-electrolyte interface tha... Read More about Sensitive detection of voltage transients using differential intensity surface plasmon resonance system.

Responsivity of the differential-intensity surface plasmon resonance instrument (2016)
Journal Article
Abayzeed, S. A., Smith, R. J., Webb, K. F., Somekh, M. G., & See, C. W. (2016). Responsivity of the differential-intensity surface plasmon resonance instrument. Sensors and Actuators B: Chemical, 235, 627-635. https://doi.org/10.1016/j.snb.2016.05.117

Surface plasmon resonance is used for the sensitive measurement of minute concentrations of bio-analytes and probing of electrochemical processes. Typical refractive index sensitivity, for the intensity approach, is around 10−6 refractive index units... Read More about Responsivity of the differential-intensity surface plasmon resonance instrument.

Condenser-free contrast methods for transmitted-light microscopy (2014)
Journal Article
Webb, K. F. (2014). Condenser-free contrast methods for transmitted-light microscopy. Journal of Microscopy, 257(1), 8-22. https://doi.org/10.1111/jmi.12181

Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser opt... Read More about Condenser-free contrast methods for transmitted-light microscopy.

Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage (2012)
Journal Article
Gunning, S. J., Chung, K. K., Donaldson, P. J., & Webb, K. F. (2012). Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. American Journal of Physiology - Cell Physiology, 303(12), C1252-C1259. https://doi.org/10.1152/ajpcell.00237.2012

The initiation of lens cataract has long been associated with the development of a membrane "leak" in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that... Read More about Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage.

Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation (2011)
Journal Article
Mendoza-Naranjo, A., Bouma, G., Pereda, C., Ramiŕez, M., Webb, K. F., Tittarelli, A., …Salazar-Onfray, F. (2011). Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation. Journal of Immunology, 187(6), 3121-3132. https://doi.org/10.4049/jimmunol.1100378

Gap junction (GJ) mediates intercellular communication through linked hemichannels from each of two adjacent cells. Using human and mouse models, we show that connexin 43 (Cx43), the main GJ protein in the immune system, was recruited to the immunolo... Read More about Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation.

Regulation of lens volume: Implications for lens transparency (2009)
Journal Article
Donaldson, P. J., Chee, K. S. N., Lim, J. C., & Webb, K. F. (2009). Regulation of lens volume: Implications for lens transparency. Experimental Eye Research, 88(2), 144-150. https://doi.org/10.1016/j.exer.2008.05.011

Lens transparency is critically dependent on the maintenance of an ordered tissue architecture, and disruption of this order leads to light scatter and eventually lens cataract. Hence the volume of the fiber cells that make up the bulk of the lens ne... Read More about Regulation of lens volume: Implications for lens transparency.

Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens (2008)
Journal Article
Hu, R. G., Suzuki-Kerr, H., Webb, K. F., Rhodes, J. D., Collison, D. J., Duncan, G., & Donaldson, P. J. (2008). Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens. Experimental Eye Research, 87(2), 137-146. https://doi.org/10.1016/j.exer.2008.05.008

Extracellular ATP has been shown to mobilize intracellular Ca2+ in cultured ovine lens epithelial cells and in human lens epithelium, suggesting a role for purines in the modulation of lens transparency. In this study, we characterized the expression... Read More about Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens.

Differentiation-dependent changes in the membrane properties of fiber cells isolated from the rat lens (2008)
Journal Article
Webb, K. F., & Donaldson, P. J. (2008). Differentiation-dependent changes in the membrane properties of fiber cells isolated from the rat lens. American Journal of Physiology - Cell Physiology, 294(5), C1133-C1145. https://doi.org/10.1152/ajpcell.00315.2007

Impedance measurements in whole lenses showed that lens fiber cells possess different permeability properties to the epithelial cells from which they differentiate. To confirm these observations at the cellular level, we analyzed the membrane propert... Read More about Differentiation-dependent changes in the membrane properties of fiber cells isolated from the rat lens.

Cl− Influx into Rat Cortical Lens Fiber Cells Is Mediated by a Cl− Conductance That Is Not ClC-2 or -3 (2004)
Journal Article
Webb, K. F., Merriman-Smith, B. R., Stobie, J. K., Kistler, J., & Donaldson, P. J. (2004). Cl− Influx into Rat Cortical Lens Fiber Cells Is Mediated by a Cl− Conductance That Is Not ClC-2 or -3. Investigative Ophthalmology & Visual Science, 45(12), 4400-4408. https://doi.org/10.1167/iovs.04-0205

PURPOSE. Exposure of organ-cultured lenses to C1- channel blockers under isotonic conditions induces a localized cortical zone of extracellular space dilations. The purpose of this study was to investigate whether elongated lens fiber cells from this... Read More about Cl− Influx into Rat Cortical Lens Fiber Cells Is Mediated by a Cl− Conductance That Is Not ClC-2 or -3.