A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

Brook, Matthew S.; Wilkinson, D.J.; Mitchell, W. Kyle; Lund, Jonathan N.; Phillips, Bethan E.; Szewczyk, Nathaniel J.; Kainulainen, H.; Lensu, S.; Koch, L.G.; Britton, S.L.; Greenhaff, Paul L.; Smith, K.; Atherton, Philip J.

doi:10.1152/ajpendo.00157.2017

All Outputs (5)

Human skeletal muscle is refractory to the anabolic effects of leucine during the postprandial muscle-full period in older men (2017)
Journal Article

Leucine modulates muscle protein synthesis (MPS), with potential to facilitate accrual/maintenance of muscle mass. Animal models suggest that leucine boluses shortly after meals may prolong MPS and delay onset of a “muscle-full” state. However, the e... Read More about Human skeletal muscle is refractory to the anabolic effects of leucine during the postprandial muscle-full period in older men.

A 4-week, lifestyle-integrated, home-based exercise training programme elicits improvements in physical function and lean mass in older men and women: a pilot study (2017)
Journal Article

Background: Developing alternative exercise programmes that can alleviate certain barriers to exercise such as psychological, environmental or socio-economical barriers, but provide similar physiological benefits e.g. increases in muscle mass and str... Read More about A 4-week, lifestyle-integrated, home-based exercise training programme elicits improvements in physical function and lean mass in older men and women: a pilot study.

Physiological adaptations to resistance exercise as a function of age (2017)
Journal Article

BACKGROUND. The impact of resistance exercise training (RE-T) across the life span is poorly defined. METHODS. To resolve this, we recruited three distinct age cohorts of young (18–28 years; n = 11), middle-aged (45–55 years; n = 20), and... Read More about Physiological adaptations to resistance exercise as a function of age.

Recent developments in deuterium oxide tracer approaches to measure rates of substrate turnover: implications for protein, lipid, and nucleic acid research (2017)
Journal Article

Purpose of review: Methods that inform on dynamic metabolism that can be applied to clinical populations to understand disease progression and responses to therapeutic interventions are of great importance. This review perspective will highlight rece... Read More about Recent developments in deuterium oxide tracer approaches to measure rates of substrate turnover: implications for protein, lipid, and nucleic acid research.

A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle (2017)
Journal Article

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D2O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells wer... Read More about A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle.